Effects of dietary inclusion of Lactobacillus and inulin on growth performance, gut microbiota, nutrient utilization, and immune parameters in broilers

Wu, Xuezhuang; Wen, Zhiguo; Hua, Jinling

doi:10.3382/ps/pez166

Cited by 49 publications

(36 citation statements)

References 42 publications

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“…The current study highlighted the significance of L. acidophilus supplementation in broiler production and intestinal health. Dietary L. acidophilus supplementation also increased the BW and ADG in broilers in a previous study ( Wu et al., 2019 ). This indicated that L. acidophilus improved broiler growth .…”

Section: Discussionsupporting

confidence: 56%

“…Growth improvement after L. acidophilus supplementation may also be due to its ability to produce endogenous enzymes or growth-promoting factors by directly fermenting nutrients in the gut. This could stimulate small intestine peristalsis, boost feed digestibility and availability, and promote gut health ( Jin et al., 2000 ; Hung et al., 2012 ; Wu et al., 2019 ). Immunity stress can cause a mass of energy consumption to synthesize inflammatory factors, so it is possible that L. acidophilus reduced the immunity stress and preserved available energy for growth and maintenance.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Lactobacillus acidophilus on the growth performance, immune response, and intestinal barrier function of broiler chickens challenged with Escherichia coli O157

Yang

Zhang

et al. 2021

Poultry Science

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We studied the effects of Lactobacillus acidophilus ( L. acidophilus ) on the growth performance, intestinal morphology, barrier function, and immune response of broilers challenged with Escherichia coli O157 ( E. Coli ). A total of 360 1-day-old Cobb male broilers were tested in a 3 × 2 factorial arrangement with 3 dietary L. acidophilus levels (0, 5 × 10 8 CFU/kg, and 10 × 10 8 CFU/kg of diet) and 2 disease challenge treatments (control or E. coli challenged). Results showed that E. coli challenge decreased the ADG, ADFI, and BW of broilers from 15 to 21 d ( P < 0.05), increased the jejunum intestinal wall thickness, and significantly increased the mortality rate. E. coli challenge significantly ( P < 0.05) decreased the serum IgA and IgM contents and peripheral blood CD3+ T cell counts ( P < 0.05), increased the serum CRP, DAO, and LPS levels at 21 d; upregulated the mRNA expression of iNOS, IL-8, IL-1β in the jejunum and iNOS in the spleen, and downregulated the occludin and ZO-1 mRNA expression in the ileum at 21 d compared with uninfected birds ( P < 0.05). Dietary L. acidophilus supplementation consistently showed higher BW, ADG, ADFI, and jejunum and ileum V:C ratio at 14 d and 21 d in the presence and absence of E. coli challenge ( P < 0.05). L. acidophilus supplementation reduced the mortality rate caused by E. coli challenge ( P < 0.05), decreased the serum CRP, DAO, and LPS levels at 14 d and 21 d; upregulated the mRNA expression of occludin and ZO-1 in the jejunum and ileum, and downregulated the mRNA expression of iNOS, IL-8, and IL-1β in the jejunum in E. coli challenged birds at 21 d ( P < 0.05). Dietary supplementation with L. acidophilus can improve the growth performance, intestinal health, and survival of broilers challenged with E. coli .

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Effects of Lactobacillus acidophilus on the growth performance, immune response, and intestinal barrier function of broiler chickens challenged with Escherichia coli O157

Yang

Zhang

et al. 2021

Poultry Science

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“…However, the positive effect of inulin administered both traditionally as a feed additive (5) and in ovo at the stage of embryonic development (6) has been repeatedly demonstrated. The administration of inulin affects the modulation of the intestinal microbiota by promoting beneficial Lactobacillus and Bifidobacterium bacteria strains, and also by inhibiting the development of pathogenic microorganisms (7,8).…”

Section: Introductionmentioning

confidence: 99%

Molecular Response in Intestinal and Immune Tissues to in Ovo Administration of Inulin and the Combination of Inulin and Lactobacillus lactis Subsp. cremoris

et al. 2021

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Intestinal microbiota are a key factor in maintaining good health and production results in chickens. They play an important role in the stimulation of immune responses, as well as in metabolic processes and nutrient digestion. Bioactive substances such as prebiotics, probiotics, or a combination of the two (synbiotic) can effectively stimulate intestinal microbiota and therefore replace antibiotic growth promoters. Intestinal microbiota might be stimulated at the early stage of embryo development in ovo. The aim of the study was to analyze the expression of genes related to energy metabolism and immune response after the administration of inulin and a synbiotic, in which lactic acid bacteria were combined with inulin in the intestines and immune tissues of chicken broilers. The experiment was performed on male broiler chickens. Eggs were incubated for 21 days in a commercial hatchery. On day 12 of egg incubation, inulin as a prebiotic and inulin with Lactobacillus lactis subsp. cremoris as a synbiotic were delivered to the egg chamber. The control group was injected with physiological saline. On day 35 post-hatching, birds from each group were randomly selected and sacrificed. Tissues (spleen, cecal tonsils, and large intestine) were collected and intended for RNA isolation. The gene panel (ABCG8, HNF4A, ACOX2, APBB1IP, BRSK2, APOA1, and IRS2) was selected based on the microarray dataset and biological functions of genes related to the energy metabolism and immune responses. Isolated RNA was analyzed using the RT-qPCR method, and the relative gene expression was calculated. In our experiment, distinct effects of prebiotics and synbiotics following in ovo delivery were manifested in all analyzed tissues, with the lowest number of genes with altered expression shown in the large intestines of broilers. The results demonstrated that prebiotics or synbiotics provide a potent stimulation of gene expression in the spleen and cecal tonsils of broiler chickens. The overall number of gene expression levels and the magnitude of their changes in the spleen and cecal tonsils were higher in the group of synbiotic chickens compared to the prebiotic group.

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“…has been extensively studied [7,14,15]. The inclusion of Lactobacillus in broiler feed improves the growth of indigenous beneficial bacteria and the growth performance of broilers [16]. Compared with salinomycin treatment, the feeding of Lactobacillus probiotics increases the jejunal villus height and reduces the villus crypt depth in broilers [17].…”

Section: Introductionmentioning

confidence: 99%

Association of Probiotic Supplementation with Improvements in the Gut Microbes, Blood Lipid Profile and Caecal Villus Morphology of Broilers

et al. 2021

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The effects of probiotic supplementation vary among species or strains, and the dose and feeding timeline could alter the intestinal environment of broilers. A fresh culture of Bacillus subtilis CE330 and multiple lactic acid bacteria (LAB) strains was administered to hatched broilers to investigate their synergistic and long-term effects. One-day-old Cobb broilers (n 30) were randomly distributed into five groups (six chicks per group) and orally fed dH 2 O (control), B. subtilis CE330, or mixed cultures of B. subtilis CE330 and LAB at different doses and times. The simultaneous oral feeding B. subtilis CE330 and 4 LAB strains (ratio equal to 1:4) resulted in the best improvements in growth and health performance in broilers raised without antibiotics. The probiotic-fed group showed a 32.3% higher body weight gain and a 30.2% increase in high-density lipids. One of the 22 Bacillus-like isolates showed similar properties to B. subtilis CE330, including inhibition of bacterial pathogens, presence of the subtilosin gene, digestive enzyme activity, and antibiotic susceptibility. PCR-DGGE analysis revealed that the control group exhibited a diverse bacterial population of pathogen-related species that was not detected in the caecum of the B. subtilis CE330-fed group. The villus number and the morphology of the epithelial cells in the caecum were obviously increased and improved the nutrient adsorption in the probiotic-fed groups. The administration of three doses of a probiotic mixture early after hatching was sufficient for a long-term health benefit on broilers. The most effective probiotics could be further developed for the production of antibiotic-free broilers. Keywords Bacillus subtilis • Lactic acid bacteria • Probiotics • Broilers • Caecal villi • PCR-DGGE Abbreviations CA4 Enterococcus faecium CA4 CE330 Bacillus subtilis CE330 CH24 Lactobacillus salivarius CH24 CH33 Enterococcus durans CH33 SH8 Pediococcus acidilactici SH8 BS Bacillus subtilis CE330 B Wilailak Siripornadulsil

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Effects of dietary inclusion of Lactobacillus and inulin on growth performance, gut microbiota, nutrient utilization, and immune parameters in broilers

Cited by 49 publications

References 42 publications

Effects of Lactobacillus acidophilus on the growth performance, immune response, and intestinal barrier function of broiler chickens challenged with Escherichia coli O157

Effects of Lactobacillus acidophilus on the growth performance, immune response, and intestinal barrier function of broiler chickens challenged with Escherichia coli O157

Molecular Response in Intestinal and Immune Tissues to in Ovo Administration of Inulin and the Combination of Inulin and Lactobacillus lactis Subsp. cremoris

Association of Probiotic Supplementation with Improvements in the Gut Microbes, Blood Lipid Profile and Caecal Villus Morphology of Broilers

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