Effects of dietary glucose supplementation on the fasted plasma metabolome in cats and dogs

Allaway, David; Kamlage, Beate; Gilham, Matthew; Hewson-Hughes, Adrian K.; Wiemer, J.; Colyer, Alison; Rein, Dietrich

doi:10.1007/s11306-013-0527-8

Cited by 27 publications

(27 citation statements)

References 52 publications

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“…Two types of MS analyses were applied to serum and EDTA plasma samples: GC-MS (Agilent 6890 GC connected to an Agilent 5973 MS system) and LC-MS/MS (Agilent 1100 high-performance liquid chromatography system coupled to an Applied Biosystems API4000 MS/MS System) were used for MxP ® Broad Profiling, as described elsewhere [ 42 , 43 , 44 ]. In brief, proteins were removed from samples by precipitation using three volumes of acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

Impact of Prolonged Blood Incubation and Extended Serum Storage at Room Temperature on the Human Serum Metabolome

et al. 2018

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Metabolomics is a powerful technology with broad applications in life science that, like other -omics approaches, requires high-quality samples to achieve reliable results and ensure reproducibility. Therefore, along with quality assurance, methods to assess sample quality regarding pre-analytical confounders are urgently needed. In this study, we analyzed the response of the human serum metabolome to pre-analytical variations comprising prolonged blood incubation and extended serum storage at room temperature by using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) -based metabolomics. We found that the prolonged incubation of blood results in a statistically significant 20% increase and 4% decrease of 225 tested serum metabolites. Extended serum storage affected 21% of the analyzed metabolites (14% increased, 7% decreased). Amino acids and nucleobases showed the highest percentage of changed metabolites in both confounding conditions, whereas lipids were remarkably stable. Interestingly, the amounts of taurine and O-phosphoethanolamine, which have both been discussed as biomarkers for various diseases, were 1.8- and 2.9-fold increased after 6 h of blood incubation. Since we found that both are more stable in ethylenediaminetetraacetic acid (EDTA) blood, EDTA plasma should be the preferred metabolomics matrix.

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Section: Methodsmentioning

confidence: 99%

Impact of Prolonged Blood Incubation and Extended Serum Storage at Room Temperature on the Human Serum Metabolome

et al. 2018

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“…Two types of mass spectrometry analyses were applied to all samples. GC-MS (gas chromatography-mass spectrometry; Agilent 6890 GC coupled to an Agilent 5973 MS-System, Agilent, Waldbronn, Germany) and LC-MS/MS (liquid chromatography-MS/MS; Agilent 1100 HPLC-System (Agilent, Waldbronn, Germany) coupled to an Applied Biosystems API4000 MS/MS-System (Applied Biosystems, Darmstadt, Germany)) were used for MxP® Broad Profiling [ 45 , 46 ]. The sample preparation process was optimized for this specific tissue type previously to enable this study.…”

Section: Methodsmentioning

confidence: 99%

Integration of tissue metabolomics, transcriptomics and immunohistochemistry revealsERG-and gleason score-specific metabolomic alterations in prostate cancer

Meller

Meyer²,

Bethan³

et al. 2015

Oncotarget

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Integrated analysis of metabolomics, transcriptomics and immunohistochemistry can contribute to a deeper understanding of biological processes altered in cancer and possibly enable improved diagnostic or prognostic tests. In this study, a set of 254 metabolites was determined by gas-chromatography/liquid chromatography-mass spectrometry in matched malignant and non-malignant prostatectomy samples of 106 prostate cancer (PCa) patients. Transcription analysis of matched samples was performed on a set of 15 PCa patients using Affymetrix U133 Plus 2.0 arrays. Expression of several proteins was immunohistochemically determined in 41 matched patient samples and the association with clinico-pathological parameters was analyzed by an integrated data analysis. These results further outline the highly deregulated metabolism of fatty acids, sphingolipids and polyamines in PCa. For the first time, the impact of the ERG translocation on the metabolome was demonstrated, highlighting an altered fatty acid oxidation in TMPRSS2-ERG translocation positive PCa specimens. Furthermore, alterations in cholesterol metabolism were found preferentially in high grade tumors, enabling the cells to create energy storage. With this integrated analysis we could not only confirm several findings from previous metabolomic studies, but also contradict others and finally expand our concepts of deregulated biological pathways in PCa.

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“…Two types of mass spectrometry analyses were applied to all samples: gas chromatography-mass spectrometry (GC/MS) and liquid chromatography-MS/MS for broad profiling as described previously [ 21 ]. Briefly, proteins were removed from plasma samples by precipitation prior to collection of polar and nonpolar fractions for both GC-MS and LC-MS/MS.…”

Section: Methodsmentioning

confidence: 99%

Glyoxylate, a New Marker Metabolite of Type 2 Diabetes

Nikiforova

Giesbertz

Wiemer

et al. 2014

Journal of Diabetes Research

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Type 2 diabetes (T2D) is characterized by a variety of metabolic impairments that are closely linked to nonenzymatic glycation reactions of proteins and peptides resulting in advanced glycation end-products (AGEs). Reactive aldehydes derived from sugars play an important role in the generation of AGEs. Using metabolite profiling to characterize human plasma from diabetic versus nondiabetic subjects we observed in a recent study that the reactive aldehyde glyoxylate was increased before high levels of plasma glucose, typical for a diabetic condition, could be measured. Following this observation, we explored the relevance of increased glyoxylate in diabetic subjects and in diabetic C57BLKS/J-Leprdb/db−/− mice in the pathophysiology of diabetes. A retrospective study using samples of long-term blood donors revealed that glyoxylate levels unlike glucose levels became significantly elevated up to 3 years prior to diabetes diagnosis (difference to control P = 0.034). Elevated glyoxylate levels impact on newly identified mechanisms linking hyperglycemia and AGE production with diabetes-associated complications such as diabetic nephropathy. Glyoxylate in its metabolic network may serve as an early marker in diabetes diagnosis with predictive qualities for associated complications and as potential to guide the development of new antidiabetic therapies.

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Effects of dietary glucose supplementation on the fasted plasma metabolome in cats and dogs

Cited by 27 publications

References 52 publications

Impact of Prolonged Blood Incubation and Extended Serum Storage at Room Temperature on the Human Serum Metabolome

Impact of Prolonged Blood Incubation and Extended Serum Storage at Room Temperature on the Human Serum Metabolome

Integration of tissue metabolomics, transcriptomics and immunohistochemistry revealsERG-and gleason score-specific metabolomic alterations in prostate cancer

Glyoxylate, a New Marker Metabolite of Type 2 Diabetes

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