Effects of dietary four different woody forages on gut microbiota of Nile tilapia ( Oreochromis niloticus )

Abstract

The present study investigated for the first time the gut bacterial responses of Nile tilapia (Oreochromis niloticus) to four different woody forages (Moringa oleifera, fermented M. oleifera, Folium mori and fermented F. mori meals) by high‐throughput Illumina sequencing. Forty‐eight bacterial phyla and a very different bacterial community consisted of Paeniclostridium, Clostridium, Mycobacterium, Synechococcus, Cetobacterium, Trachydiscus and Romboutsia were detected in this study. Interestingly, it was found that the number of bacterial operational taxonomic units (OTUs) in foreguts and hindguts of Nile tilapia in reference diet group was very close, while the number of bacterial OTUs in hindguts was much more than that in foregut of tilapia after fed with different woody forages. These results indicated that woody forages could effectively enhance the diversity and difference of gut bacterial community of tilapia. Furthermore, woody forges might influence the distribution of some unique bacterial community (Desulfovibrio, Thiobacillus, Methylobacillus, Marmoricola and Syntrophobacter) in guts of Nile tilapia, which was confirmed by comparing predicted gut bacterial functions that two pathways including membrane transport and carbon metabolism in four woody forage groups were significantly higher than those in reference diet group. This present study contributed to our understanding of the gut bacterial diversity and functions of Nile tilapia fed with different woody forages.

Role of Gastrointestinal Microbiota from Crucian Carp in Microbial Transformation and Estrogenicity Modification of Novel Plastic Additives

“…The Protebacteria phylum was identified as the most prevalent member (>96% of the total bacteria) in both GI cultures, where the unclassified Enterobacteriaceae , Desulfovibrio , and Klebsiella taxa were the two most abundant genera with higher proportions (Figures and S11). The distributions of the major phyla ( Firmicutes , Bacteroidetes , and Proteobacteria ) are consistent with previous gut studies of fish and other aquatic animals, indicating constant core gut bacteria across a broader range of species. ,− Enterobacteriaceae was also reported as the common amylolytic and lipolytic bacteria in GI tract, while Klebsiella and Desulfovibrio were the representative genera exhibiting high abundance in freshwater fish gut. , Fungal communities remained stable throughout the enrichment process. The most abundant phylum was Ascomycota in all samples (>62.8%, Figure S10), which was also found as a typical heterotrophic fungi tax in fish gut from previous studies. , Aureobasidium , Fusarium , and Aspergillus were the most prominent group in the Ascomycota phylum at the genus level (Figure ).…”

“…29,69−71 Enterobacteriaceae was also reported as the common amylolytic and lipolytic bacteria in GI tract, 70 while Klebsiella and Desulfovibrio were the representative genera exhibiting high abundance in freshwater fish gut. 72,73 Fungal communities remained stable throughout the enrichment process. The most abundant phylum was Ascomycota in all samples (>62.8%, Figure S10), which was also found as a typical heterotrophic fungi tax in fish gut from previous studies.…”

Grazing Effects of Xenocypris davidi Bleeker (Cyprinidae, Cypriniformes) on Filamentous Algae and the Consequent Effects on Intestinal Microbiota

“…Mycobacterium, which belongs to Actinobacteria, is a pathogen of fsh and human coinfection [84][85][86]. Zhang et al [87] reported that the content of Mycobacterium in the intestines of Nile tilapia (Oreochromis niloticus) is abundant when fed with diferent woody forages. According to the correlation analysis of this experiment, it has been proven that a high-carbohydrate diet promotes Mycobacterium booming.…”

Biodiversity and antimicrobial activity of intestinal fungi from three species of coral reef fishes

“…After each sh was cut in an arc along the anus in an upward direction with dissecting scissors, its intestine was separated and divided into fore-, mid-and hind-intestinal segments with dissecting scissors (Wu et al 2020). Then the mucosa (including intestinal contents) of each intestinal segment was individually collected with sterile dissecting scissors, and nally, 0.5 g of the mucosa was gently scraped into a centrifuge tube (Zhang et al 2021). Then, 0.5 mL of each of the three seawater samples was diluted into 4.5 mL of sterile seawater.…”