Effects of dietary forage particle size and concentrate level on fermentation profile, in vitro degradation characteristics and concentration of liquid- or solid-associated bacterial mass in the rumen of dairy cows

Zebeli, Qendrim; Tafaj, M.; Weber, I.; Steingaß, H.; Drochner, W.

doi:10.1016/j.anifeedsci.2007.04.002

Cited by 39 publications

(66 citation statements)

References 38 publications

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“…Both methods are described by Tafaj et al (2004a). For measurement of ammonia concentration in PARL and FRL, two replicates of supernatant (each of 5 ml) were steam distilled (Gerhardt, Bonn, Germany) as described by Zebeli et al (2008). For protozoal enumeration, 1 ml of sample from PARL and FRL was added to 9 ml of methyl green: formalin: saline (MFS) solution (Ogimoto and Imai 1981) and stored at 48C until counted in a Fuchs-Rosenthal chamber.…”

Section: Digesta Sampling Ruminal Fermentation Profile and Protozoa mentioning

confidence: 99%

“…To isolate liquid-associated bacteria, 1000 ml of ruminal liquid phase fluid was collected from each cow from the ventral rumen using the technique described by Zebeli et al (2008). Immediately after sampling, digesta samples were placed in ice and maintained until further processing.…”

Section: Isolation Of Mixed Bacterial Massmentioning

confidence: 99%

“…Kononoff and Heinrichs 2003;Beauchemin 2006a, 2006b;Zebeli et al 2008), the determination of an optimum forage particle length for diets of dairy cows was difficult, particularly due to the inconsistency of obtained results. For example, forage particle length was reported to affect chewing activity, ruminal pH and microbial activity in some studies (Beauchemin et al 2003;Yang and Beauchemin 2006a), but failed to have an effect in other studies (Kononoff and Heinrichs 2003;Yang and Beauchemin 2006b;Zebeli et al 2008).…”

Section: Introductionmentioning

confidence: 98%

“…Characteristics of cows, ingredients and chemical composition of total mixed rations differing in theoretical particle length of grass silage (Means + SD; n ¼ 3). sunflower kernels and 3% vitamin-mineral premix (grains were ground to pass a 3-mm screen): Chemical composition of diets was corrected for feed refusals; # Non-fibre-carbohydrates ¼ 100 7 (NDF þ CP þ ether extracts þ ash); { Determined based on chemical composition and 24 h gas production of feeds (Hohenheim gas test; Menke and Steingass 1988); { Fractional rate of 72-h gas production, used as an index for fractional DM degradation rate (Zebeli et al 2008); x Measured by Penn State Particle Separator (Lammers et al 1996); { peNDF 48 ¼ physically effective NDF measured as the NDF content of the diet multiplied by the amount of DM 4 8 mm (Lammers et al 1996).…”

Section: Introductionmentioning

confidence: 99%

“…The ingredients and chemical composition of the diets are presented in Table 1. The degradation characteristics of ingredients and TMR were determined based on cumulative in vitro gas production (incubation time 72 h) using the Hohenheim Gas Test, as described by Zebeli et al (2008).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the effects of dietary particle fractions on fermentation profile and concentration of microbiota in the rumen of dairy cows fed grass silage-based diets

Zebeli

Tafaj

Junck

et al. 2008

Archives of Animal Nutrition

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The study evaluated the effects of three different theoretical particle lengths (TPL) of grass silage on the distribution of particle fractions of the diet and the resulting effects on fermentation profile and concentrations of protozoa and mixed bacterial mass in the rumen of three lactating Holstein cows fed total mixed rations (45% grass silage, 5% grass hay and 50% concentrate) ad libitum. Decreasing TPL of grass silage (long, medium, short) reduced particles retained on the 19-mm sieve of the Penn State Particle Separator, while particle fractions from 8 mm to 19 mm and smaller than 8 mm were increased. Different TPL did not affect pH and the concentration of volatile fatty acids in the rumen. However, lowering the TPL from long to medium increased significantly the bicarbonate concentration, acetate proportion and protozoal number in the rumen, whereas the proportion of bacterial protein in ruminal digesta and its amino acid concentration were significantly increased by the short TPL. For the current feeding conditions, it can be concluded that increasing the fraction of particles between 8 and 19 mm and probably even the fraction below 8 mm by decreasing TPL of grass silage do not adversely affect rumen conditions and can be beneficial in terms of optimising concentration and activity of ruminal microbiota in high-yielding dairy cows.

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Section: Digesta Sampling Ruminal Fermentation Profile and Protozoa mentioning

confidence: 99%

Section: Isolation Of Mixed Bacterial Massmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of the effects of dietary particle fractions on fermentation profile and concentration of microbiota in the rumen of dairy cows fed grass silage-based diets

Zebeli

Tafaj

Junck

et al. 2008

Archives of Animal Nutrition

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Effect of Caragana korshinskii Kom. as a partial substitution for sheep forage on intake, digestibility, growth, carcass features, and the rumen bacterial community

Wang

Huang

Zhang

et al. 2022

Trop Anim Health Prod

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The aim of this study was to verify that Caragana korshinskii Kom. (CK) as a component of sheep forage influences lamb digestibility and rumen fermentation by altering the rumen microbial community. Hence, 12 female Tan sheep were allocated into 2 groups: receiving (CK group) or not (control group) 10% of the diet forage fraction with CK. During the 60-day experiment, growth performance, apparent digestibility, rumen volatile fatty acids (VFAs), and nitrogen balance were measured. Meanwhile, the rumen bacterial community diversity and composition were detected by the 16S rRNA sequence. The results indicated that the apparent digestibility of acid detergent fibre (ADF) tended to be higher (0.05 < P < 0.10), and the feed conversion efficiency was improved (P < 0.05) when CK was offered. Compared to those under alfalfa, the composition and abundance of the rumen microbial community were altered in the CK group, and the phylum Firmicutes, which is involved in promoting fibre digestion, increased in abundance. Moreover, VFAs tended to decrease (0.05 < P < 0.10), and the molar proportion of butyrate declined; similarly, levels of hypoxanthine and xanthine were lower (P < 0.05) in the sheep fed CK and may have been responsible for the decreased abundance of Fibrobacter spp., which are cellulolytic ruminal bacteria associated with VFA production.

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Impact of subacute ruminal acidosis on the diversity of liquid and solid-associated bacteria in the rumen of goats

Huo

Zhu

Mao

2013

World J Microbiol Biotechnol

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This study was aimed to investigate the impact of subacute ruminal acidosis (SARA) on the diversity of liquid (LAB) and solid-associated bacteria (SAB) following high-grain feeding. Six ruminally cannulated goats were divided into two groups: one group was fed a hay diet (COD), and the other group was fed a high grain diet (SAID). Rumen liquids and rumen solids were sampled after 2 weeks adaption. SARA was diagnosed with a pH below 5.8 for 8 h. SAID decreased ruminal pH (P < 0.001) and increased the acetate (P = 0.017), propionate (P = 0.001), butyrate (P < 0.001) and total volatile fatty acid (P < 0.001) concentration in rumen compared with the COD. Denaturing gradient gel electrophoresis fingerprints analysis revealed a clear separation between both the diet and the fraction of rumen digesta in bacterial communities. Pyrosequencing analysis showed that the proportion of phylum Bacteroidetes in the SAID-LAB and SAID-SAB communities was less than in the COD group, whereas the SAID group had a greater percentage of Firmicutes in both the LAB and SAB libraries. UniFrac analyses and a Venn diagram revealed a large difference between the two diets in the diversity of rumen bacterial communities. Overall, our findings revealed that SARA feeding did alter the community structure of rumen liquids and rumen solids. Thus, manipulation of dietary factors, such as ratio of forage to concentrate may have the potential to alter the microbial composition of rumen liquid and rumen solid.

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Effects of dietary forage particle size and concentrate level on fermentation profile, in vitro degradation characteristics and concentration of liquid- or solid-associated bacterial mass in the rumen of dairy cows

Cited by 39 publications

References 38 publications

Evaluation of the effects of dietary particle fractions on fermentation profile and concentration of microbiota in the rumen of dairy cows fed grass silage-based diets

Evaluation of the effects of dietary particle fractions on fermentation profile and concentration of microbiota in the rumen of dairy cows fed grass silage-based diets

Effect of Caragana korshinskii Kom. as a partial substitution for sheep forage on intake, digestibility, growth, carcass features, and the rumen bacterial community

Impact of subacute ruminal acidosis on the diversity of liquid and solid-associated bacteria in the rumen of goats

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