Effects of dietary fish oil supplementation on membrane fluidity and enzyme activity in rat small intestine

Stenson, William F.; Seetharam, Bellur; Talkad, Venugopal D.; Pickett, Walter C.; Dudeja, Pradeep K.; Brasitus, Thomas A.

doi:10.1042/bj2630041

Cited by 51 publications

(24 citation statements)

References 27 publications

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“…McCarthy et al [26] also reported that feeding a CO-rich diet was associated with low levels of the disaccha ridases in rats. Mucosal sucrase activity was decreased by feeding a diet containing 9% FO compared to safflower oil (n-6 fatty acids) [27] , In contrast, Stenson et al [19] reported similar sucrase and lactase activities in rats kept on chow or FO diet. Triglycerides in FO may be hydrolyzed less efficiently by pan creatic lipases than other triglycerides [28], Undigested fat affects digestion and absorp tion of other nutrients coexisting in the food [29].…”

Section: Resultsmentioning

confidence: 89%

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Dietary Fat Effects on Brush Border Membrane Composition and Enzyme Activities in Rat Intestine

Kaur

Ojha

et al. 1996

Ann Nutr Metab

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The effect of dietary fats on the chemical composition and enzyme activities has been studied in intestinal brush border membranes (BBM) of rats. Animals were given commercial rat pellet diet (RP) or semisynthetic diet rich in either saturated [coconut oil (CCO)] or polyunsaturated [n-6, corn oil (CO) or n-3, fish oil (FO)] fat at the 10% level for 5 weeks. The membrane cholesterol/phospholipid ratio was augmented in CO- or RP-fed rats. There was an increase in level of saturated fatty acids in BBM from CCO- or FO-fed animals, n-3 polyunsaturated fatty acid content was raised in FO-fed rats, while the proportion of linoleic acid and arachidonic acid was enhanced in animals given a CO diet. Membrane fluidity was in the order of CCO < RP = CO < FO. The membrane hexose content was high (p < 0.05) in the CCO group. Hexosamines were elevated (p < 0.05) in CCO- or FO-fed rat brush borders. Membrane fucose was unaltered, while sialic acid content was elevated in CO- (p < 0.05) and FO- (p < 0.01) fed vs. CCO-fed rats. Lectin binding to brush borders corroborated these findings. The activities of alkaline phosphatase, sucrase and lactase were augmented (p < 0.001) in CCO-fed animals. Leucine-aminopeptidase and sucrase activities were depressed by FO feeding. The activities of PNP-β-glycosidases were the highest in FO-fed rats. These results indicate that dietary fat quality markedly affects microvillus membrane lipid composition, glycosylation and enzyme functions in rat intestine.

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Section: Resultsmentioning

confidence: 89%

“…It is reported that corn-oil-fed BBM are more fluid whereas fishoil-fed membranes are less fluid compared to the membranes from butter fat/lard-fed ani mals [6,7], On the other hand. Stenson et al [19] reported that FO supplementation did not change the biophysical parameters of the membranes.…”

Section: Resultsmentioning

confidence: 99%

Dietary Fat Effects on Brush Border Membrane Composition and Enzyme Activities in Rat Intestine

Kaur

Ojha

et al. 1996

Ann Nutr Metab

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“…Controversy exists in terms of serum palmitoleate modifications after fish oil intake because of its distribution in different lipids. The content of palmitoleate in lysophophatidic acid, phopsholipids, and other fractions results increased or decreased in different studies and species (44)(45)(46), with no general conclusions.…”

Section: Dietary Palmitoleatementioning

confidence: 99%

The Role of the Novel Lipokine Palmitoleic Acid in Health and Disease

Frigolet

Gutiérrez-Aguilar

2017

Advances in Nutrition

186

143

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The monounsaturated fatty acid palmitoleate (palmitoleic acid) is one of the most abundant fatty acids in serum and tissues, particularly adipose tissue and liver. Its endogenous production by stearoyl-CoA desaturase 1 gives rise to its cis isoform, cis-palmitoleate. Although trans-palmitoleate is also synthesized in humans, it is mainly found as an exogenous source in ruminant fat and dairy products. Recently, palmitoleate was considered to be a lipokine based on evidence demonstrating its release from adipose tissue and its metabolic effects on distant organs. After this finding, research has been performed to determine whether palmitoleate has beneficial effects on metabolism and to elucidate the underlying mechanisms. Thus, the aim of this work was to review the current status of knowledge about palmitoleate, its metabolism, and its influence on metabolic abnormalities. Results have shown mixed cardiovascular effects, direct or inverse correlations with obesity, and hepatosteatosis, but a significant amelioration or prevention of insulin resistance and diabetes. Finally, the induction of palmitoleate release from adipose tissue, dietary intake, and its supplementation are all interventions with a potential impact on certain metabolic diseases. Adv Nutr 2017;8(Suppl):173S-81S.

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“…Changes in the fatty acid composition of the membrane lipid environment can modify membrane fluidity and thereby alter the function of membrane receptors [3,4] and membrane-bound enzymes [5], thus dietary fatty acids have the potential to exert profound effects on human physiology. However, although a number of studies have shown that dietary supplementation with fish oil or whole fish can alter erythrocyte phospholipid composition in humans [6][7][8], the effects on membrane composition and fluidity of the relatively modest changes in membrane fatty acids which can be achieved with conventional foods or low doses of supplements are not yet clear.…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Fish Oil Supplementation on the Phospholipid Composition and Fluidity of Cell Membranes from Human Volunteers

Lund

Harvey²,

Ladha³

et al. 1999

Ann Nutr Metab

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Background: Membrane fluidity is an important aspect of cellular physiology which may be manipulated by diet. Methods: We studied the effect of dietary fish oil on the membrane composition of erythrocytes and cheek cells, and on membrane fluidity of erythrocytes as assessed by fluorescence recovery after photobleaching (FRAP). Healthy volunteers received a daily supplement of fish oil (930 mg EPA, 630 mg DHA) for 42 days. Results: The intervention reduced the ratio of n-6 to total fatty acid in the phospholipid fraction of erythroctye membranes but the n-3 fraction remained stable and the ratio of cholesterol to phospholipid increased. The level of EPA and DHA in cheek cells increased significantly during the intervention period. The mean diffusion coefficient of the fluorescent probe in erythrocyte membranes increased from 7.2 ± 0.7 × 10^–9 cm²/s at the start to 9.8 ± 0.5 × 10^–9 cm²/s after 21 days. Membrane fluidity remained higher than the initial value 42 days after withdrawal of the supplement. Fish oil also reduced platelet aggregation in response to ADP but there was no effect on plasma lipid profiles. Conclusion: We conclude that n-3 fatty acids influence erythrocyte membrane composition at relatively low levels of supplementation by a mechanism which does not necessarily involve an increase in the level of EPA or DHA present in the membrane.

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Effects of dietary fish oil supplementation on membrane fluidity and enzyme activity in rat small intestine

Cited by 51 publications

References 27 publications

Dietary Fat Effects on Brush Border Membrane Composition and Enzyme Activities in Rat Intestine

Dietary Fat Effects on Brush Border Membrane Composition and Enzyme Activities in Rat Intestine

The Role of the Novel Lipokine Palmitoleic Acid in Health and Disease

Effects of Dietary Fish Oil Supplementation on the Phospholipid Composition and Fluidity of Cell Membranes from Human Volunteers

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