Effects of dietary docosahexaenoic acid sources, microalgae meal and oil, on growth, fatty acid composition and docosahexaenoic acid retention of orange-spotted grouper,Epinephelus coioides

Abstract

This study evaluated the effects of Aurantiochytrium spp. microalgae meal and oil as dietary docosahexaenoic acid (DHA) sources on the growth, fatty acid composition and DHA retention of orange-spotted grouper, Epinephelus coioides. Dietary fish oil was replaced with microalgae meal or oil to provide an equal amount of DHA as a fish oil-containing basal diet. In total, three experimental diets were fed to triplicate groups of fish (initial wt: 8.48 AE 0.06 g) in a recirculating system for 8 weeks. The weight gain and feed efficiency of the fish did not differ significantly among the experimental diets. The fatty acid composition of the whole body of the fish generally reflected the composition of their diet. The concentration of eicosapentaenoic acid in the whole body was higher in the fish fed the fish meal control diet than in those fed the two experimental diets The fish fed the control diet and those fed the diet containing microalgae oil exhibited higher DHA concentrations than did the fish fed the diet containing microalgae meal. The whole-body DHA retention was the highest in the fish fed the diet with microalgae oil, followed by the fish fed the control diet. The lowest whole-body DHA retention was observed in the fish fed the diet containing microalgae meal. The results suggested that the oil from Aurantiochytrium spp. microalgae can be used as DHA source for the grouper. DHA utilization by the fish was higher when the diet was supplemented with microalgae oil than with dry microalgae meal.

Effects of complete replacement of fish oil with plant oil mixtures and algal meal on growth performance and fatty acid composition in juvenile yellowtail Seriola quinqueradiata

“…The complete replacement of FO using Aurantiochytrium sp. oil/meal has been undertaken in salmonids (Miller et al 2007;Kousoulaki et al 2015), tilapia (Sarker et al 2016;Brignol et al 2018), olive flounder Paralichthys olivaceus (Qiao et al 2014), giant grouper Epinephelus lanceolatus (García-Ortega et al 2016), and orange-spotted grouper E. coioides (Lee et al 2017). The absence of a negative effect was also confirmed on the growth performance of longfin yellowtail fed with an algal meal (AM)-supplemented fish meal (FM)-based diet and FMreplaced diets .…”

Effects of dietary replacement of fish oil with soybean oil on the growth performance, plasma components, fatty acid composition and lipid metabolism of groupers Epinephelus coioides

“…Over the past two decades, there has been great research progress on grouper nutrition and feeds (Feng et al, 2020). In terms of the dietary utilization of lipid/FAs in fish species, a series of reports have been published (An et al, 2020;Chen et al, 2017;Lee et al, 2018;Li, Mai, et al, 2016;Luo et al, 2005). So far, however, there are few studies on the utilization effect of VOs used to substitute FO in grouper diets (Lin et al, 2007;Tseng & Lin, 2020;Yan et al, 2020).…”

Effect of dietarySchizochytriumsp. oil as an n‐3 long‐chain polyunsaturated fatty acid source on growth performance, lipid metabolism and antioxidant status in juvenile grass carp (Ctenopharyngodon idellus): A comparative study with fish oil

“…Studies of Sc oil in marine fish have been reported in Atlantic salmon and Pacific white shrimp (Miller, Nichols, & Carter, 2007; Nonwachai, Purivirojkul, Chuchird, & Limsuwan, 2009), while there were few studies on Sc oil in freshwater fish feed. In the study of orange‐spotted grouper ( Epinephelus coioides ), it was pointed out that the retention of DHA in microalgae oil by fish was higher than that of dry microalgae meal, indicating that the cell wall contained in microalgae meal affected the absorption of DHA, and removal of cell walls could improve the utilization of DHA‐rich microalgae (Lee, Zhuo, & Lin, 2018). This study indicated that adequate dietary n‐3LC PUFA supplementation significantly improved growth performance of grass carp.…”