Effects of dietary butter enrichment on the fatty acid distribution of phospholipid fractions isolated from rat platelets and aortae

Naughton, Joan M.; Sinclair, Andrew J.; O’Dea, Kerin; Steel, M.S.

doi:10.1016/0005-2760(88)90155-5

Cited by 23 publications

(9 citation statements)

References 23 publications

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“…The results on asymmetry of fluidity in platelet membranes obtained in this study are reasonable because in mammalian blood cells, phospholipids present in the inner leaflet of plasma membranes and granule membranes contain much higher concentrations of polyunsaturated fatty acids than those in the outer layer (Williams, Kuchmak & Witter, 1966;Lagarde et al, 1982;Naughton et al, 1988). The distribution of cholesterol, which has been suggested to be present mainly in the outer layer in erythrocytes (Fisher, 1976;Hale & Schroeder, 1982), should also be cosidered.…”

Section: Discussionsupporting

confidence: 67%

Asymmetry of membrane fluidity in the lipid bilayer of blood platelets: Fluorescence study with diphenylhexatriene and analogs

et al. 1991

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Membrane fluidity of bovine platelets was examined with diphenylhexatriene (DPH), its cationic trimethylammonium derivative (TMA-DPH) and anionic propionic acid derivative (DPH-PA). After addition of these probes to platelet suspensions at 37 degrees C, the fluorescence intensity of DPH-PA reached equilibrium within 2 min, whereas those of DPH and TMA-DPH increased gradually. With increase in the fluorescence intensity of TMA-DPH, its fluorescence anisotropy decreased significantly, but the fluorescence anisotropies of DPH-PA and DPH did not change during incubation. The gradual increase of fluorescence intensity of TMA-DPH was due to its penetration into the cytoplasmic side of the platelet membrane, as shown quantitatively by monitoring decrease in its extractability with albumin. Transbilayer movement of TMA-DPH was markedly temperature-dependent, and was scarcely observed at 15 degrees C. The fluorescence intensity of TMA-DPH was much higher in platelet membranes and vesicles of extracted membrane lipids than the initial intensity in intact platelets. Moreover, the fluorescence anisotropy of TMA-DPH was much lower in the former preparations than the initial value in intact platelets. These results suggest that binding sites for TMA-DPH in the cytoplasmic side of the platelet membrane are more fluid than those in the outer leaflet of the plasma membrane. Platelet activation by ionomycin induced specific change in the fluorescence properties of TMA-DPH without causing transbilayer incorporation of the probe.

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Section: Discussionsupporting

confidence: 67%

Asymmetry of membrane fluidity in the lipid bilayer of blood platelets: Fluorescence study with diphenylhexatriene and analogs

et al. 1991

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“…Sinclair et al . demonstrated in butter fat‐fed rats that the level of DHA was high (Naughton et al . 1988).…”

Section: What Is the Effect Of Dietary Fats That Meet The Requirementmentioning

confidence: 99%

“…Interestingly, dairy fat has very low levels of PUFA (~3%), and the LA : ALA ratio would seem to be ideal (2:1) to allow maximal endogenous synthesis of DHA. Sinclair et al demonstrated in butter fat-fed rats that the level of DHA was high (Naughton et al 1988). More important are the findings of others who have shown that infants fed formulas based on dairy fats (Sanders & Naismith 1979) or indeed evaporated milk (Courage et al 1998) have LCPUFA status midway between those fed formulas enriched with LA-rich vegetable oils and those fed breast milk.…”

Section: Lactationmentioning

confidence: 99%

Conversion of linoleic acid and alpha‐linolenic acid to long‐chain polyunsaturated fatty acids (LCPUFAs), with a focus on pregnancy, lactation and the first 2 years of life

Gibson

Mühlhäusler

Makrides

2011

Maternal & Child Nutrition

211

172

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Over the past two decades, there has been a marked shift in the fatty acid composition of the diets of industrialized nations towards increased intake of the n-6 fatty acid linoleic acid (LA, 18:2n-6), largely as a result of the replacement of saturated fats with plant-based polyunsaturated fatty acid (PUFA). While health agencies internationally continue to advocate for high n-6 PUFA intake combined with increased intakes of preformed n-3 long-chain PUFAs (LCPUFA) docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3) to reduce the incidence of cardiovascular disease (CVD), there are questions as to whether this is the best approach. LA competes with alpha-linolenic acid (18:3n-3) for endogenous conversion to the LC derivatives EPA and DHA, and LA also inhibits incorporation of DHA and EPA into tissues. Thus, high-LA levels in the diet generally result in low n-3 LCPUFA status. Pregnancy and infancy are developmental periods during which the fatty acid supply is particularly critical. The importance of an adequate supply of n-3 LCPUFA for ensuring optimal development of infant brain and visual systems is well established, and there is now evidence that the supply of n-3 LCPUFA also influences a range of growth, metabolic and immune outcomes in childhood. This review will re-evaluate the health benefits of modern Western diets and pose the question of whether the introduction of similar diets to nations with emerging economies is the most prudent public health strategy for improving health in these populations.

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“…Platelets were separated from 10 mL of blood collected in CTAD vacutainers (27) as described by Naughton et al (28), and extracted in 10 mL of 1:1 chloroform/methanol. All platelet separation steps were carried out in polypropylene or silanized glass tubes, and PL fatty acid analysis proceeded as for serum PL fatty acids.…”

Section: Subjectsmentioning

confidence: 99%

The effect of short‐term diets rich in fish, red meat, or white meat on thromboxane and prostacyclin synthesis in humans

Mann

Sinclair

Pille

et al. 1997

Lipids

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Foods which increase tissue arachidonic acid levels have been proposed to increase thrombosis tendency, presumably through increased platelet aggregation. This study examined the effect of doubling the dietary arachidonic acid (20:4n-6) using meat- or fish-based diets on the systemic production of prostacyclin (PGI2) and thromboxane (TXA2) in 29 healthy, nonsmoking adults. There were three, 3-wk low-fat dietary periods (< 15% energy as fat) in which subjects consumed a vegetarian diet for 1 wk followed by 2 wk on diets containing meat or fish as sources of 20:4n-6. Between each diet period, there was a 3-wk washout period, during which subjects returned to their normal diets. The level of 20:4n-6 consumed during the last 2 wk of each study was approximately double the usual intake (mean 140 mg/d), while the mean eicosapentaenoic acid (20:5n-3) content of the diets varied from 1 mg/d on the white meat diet to 70 mg/d on the red meat diet and to 847 mg/d on the fish diet. The serum phospholipid (PL) 20:4n-6/20:5n-3 ratios were 11:1 on the vegetarian diet, 15:1 on the white meat diet, 8:1 on the red meat diet, and 2:1 on the fish diet (P < 0.001). Neither white nor red meat diets affected platelet 20:4n-6 levels, platelet aggregation, ex vivo platelet TXB2 production, or the systemic PGI2 or TXA2 production as measured by gas chromatography-mass spectrometry analysis of the excretion levels of the principal urinary metabolites 2,3-dinor-6-keto-PGF1 alpha (PGI2-M) and 11-dehydro-TXB2 (TXA2-M), respectively. The fish diet decreased the 20:4n-6/20:5n-3 ratio in platelet PL from the baseline level of 45:1 to 13:1 (P < 0.001), had no effects on platelet aggregation, but significantly decreased platelet TXB2 production (collagen-stimulated) and TXA2-M production, while PGI2-M levels were unaltered. These results indicate that short-term diets which double the usual 20:4n-6 intake using white meat (175-330 g/d) or red meat (275-530 g/d) are not associated with an increased TXA2 production, but this does not rule out the adverse effects of 20:4n-6 at higher levels in the diet, or for more prolonged periods. Short-term diets containing fish (100-200 g/d with 90-210 mg/d 20:4n-6 and approximately 650-1000 mg/d 20:5n-3) led to significant increases in platelet 20:5n-3 levels and a decrease in the ex vivo and systemic TXA2 production.

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Effects of dietary butter enrichment on the fatty acid distribution of phospholipid fractions isolated from rat platelets and aortae

Cited by 23 publications

References 23 publications

Asymmetry of membrane fluidity in the lipid bilayer of blood platelets: Fluorescence study with diphenylhexatriene and analogs

Asymmetry of membrane fluidity in the lipid bilayer of blood platelets: Fluorescence study with diphenylhexatriene and analogs

Conversion of linoleic acid and alpha‐linolenic acid to long‐chain polyunsaturated fatty acids (LCPUFAs), with a focus on pregnancy, lactation and the first 2 years of life

The effect of short‐term diets rich in fish, red meat, or white meat on thromboxane and prostacyclin synthesis in humans

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