Effects of dietary Bopu powder supplementation on intestinal development and microbiota in broiler chickens

Abstract

This study aimed to investigate the effect of dietary supplementation with Bopu powder on intestinal development and bacterial community composition in broiler chickens. A total of 486 1-day-old arbor acres broilers were fed a basal diet (CON group), a basal diet supplemented with 50 mg/kg aureomycin (AB group), or a basal diet supplemented with 40 mg/kg Bopu powder (BP group). The results showed that the BP group had significantly lower serum tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and diamine oxidase concentrations and had significantly higher serum IL-10 concentrations than CON group (p < 0.05). Groups AB and BP had a significantly higher weight per unit length of the small intestine and villus height than the CON group (p < 0.05), and BP group had a significantly higher ratio of villus height to crypt depth than groups CON and AB (p < 0.05). Compared to the CON group, dietary Bopu powder or aureomycin supplementation significantly increased transforming growth factor-α concentration and mRNA expressions of zonula occludens-1 (ZO-1) and occludin, and decreased intestinal mucosal concentrations of TNF-α, IL-6, IL-10, caspase-3, and caspase-8 and mRNA expressions of nuclear factor-kappa-B and Bax/Bcl-2 ratio in the intestinal mucosa (p < 0.05). Meanwhile, BP group had significantly higher ZO-1, secretory immunoglobulin A, interferon-γ concentrations, and mRNA expressions of glucose transporter type-2 and sirtuin-1, and significantly lower IL-1β concentration than groups CON and AB in intestinal mucosa (p < 0.05). Dietary Bopu powder supplementation significantly increased the concentration of trefoil factor family member and mRNA expressions of superoxide dismutase-1 and bcl-2 associated X, and significantly reduced casepase-9 concentration and myeloid differentiation primary response-88 expression in the intestinal mucosa of broiler chickens relative to CON group (p < 0.05). Moreover, results of high-throughput sequencing showed that broilers in the BP group had microbial community structure distinct from that in CON group, and the addition of Bopu powder increased the abundances of Faecalibacterium and Colidextribacter (p < 0.05). Therefore, our study suggests a synergic response of intestinal development and microbiota to the Bopu powder, and provides a theoretical basis as a potential substitute for antibiotics.

Effect of Dietary <i>Gelsemium elegans</i> Benth. Extract on the Growth, Slaughter Performance, Meat Quality, Intestinal Morphology, and Microflora of Yellow-Feathered Chickens

“…Villus height, crypt depth, and the V/C ratio are important parameters for the healthy gut of broilers and are directly related to the absorptive capacity of the mucosa [36]. A higher villus height and V/C ratio are better for the intestinal absorption of nutrients and for broiler growth [37]. In the present study, 1.25 GEBE significantly decreased the ratio of duodenal villus height to crypt depth, suggesting a negative effect of GEBE on intestinal health.…”

Effects of Dietary Isoleucine Supplementation on the Production Performance, Health Status and Cecal Microbiota of Arbor Acre Broiler Chickens

“…Bacteroides enhance the body’s immunity by interacting with the host immune system [ 59 ]. Colidextribacter contributes to regulating the inflammatory response and maintains the integrity of the gut mucosa [ 21 ]. This indicated that the dietary addition of 400 mg/kg Ile affects the composition of the intestinal flora and increases the abundance of beneficial bacteria.…”

“…Then, DNA concentration and purity were measured with 1% agarose gels, and DNA was diluted to 1 ng/μL using sterile water according to the measured concentration. The V3-V4 hypervariable regions of 16S rRNA were amplified using 515F-806R primer [ 21 ]. All PCR reactions were performed with 15 μL of Phusion ® High Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, USA).…”

Macleaya cordata isoquinoline alkaloids attenuate Escherichia coli lipopolysaccharide-induced intestinal epithelium injury in broiler chickens by co-regulating the TLR4/MyD88/NF-κB and Nrf2 signaling pathways

“…The concentrations of diamine oxidase (DAO) and D-lactate in the serum were measured using ELISA kits (Jiangsu Meimian Industrial Co., Ltd.) following a previous protocol ( 12 ).…”

“…According to reports, dietary supplementation with IA improves the antioxidant capacity and promotes an increased abundance of advantageous microorganisms in the small intestine of laying hens ( 11 ). In addition, the dietary addition of 40 mg/kg Bopu powder with IA significantly affects intestinal development and function, as well as relieves intestinal inflammation and oxidative stress in broilers ( 12 ). However, it is still unclear whether the addition of Macleaya cordata IA to broiler diets can effectively mitigate the intestinal injury in broilers.…”