Effects of dietary and inhalative cadmium on hemoglobin and hematocrit in rats

Prigge, Elena-Sophie; Baumert, H.-P.; Muhle, H.

doi:10.1007/bf01685982

Cited by 21 publications

(7 citation statements)

References 7 publications

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“…Similarly, daily rat exposition to 15 mg/kg CdCl 2 for 30 days resulted in anaemia followed by the changes in the metabolism and antioxidative activity of erythrocytes (Kostic et al 1993). This effect has already been described by Prigge et al (1977) presenting rat anaemia due to increased Cd uptake in feed (up to 31 mg/kg). Paul et al (2002) reported higher arsenic accumulation in liver of iron-deficient rats compared to the animals with optimal iron uptake.…”

Section: Introductionmentioning

confidence: 63%

Long-term effect of diet amended by risk elements contaminated soils on risk element penetration and physiological parameters of rats

Vlčková¹,

Malinová²,

Koubková³

et al. 2014

CZECH J ANIM SCI

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ABSTRACT:The long-term accumulation of risk elements (As, Cd, Pb) originated from differently contaminated soils in rat organism was investigated during a model two-generation experiment. The effect of soil contamination level, gender, and length of exposure as well as the interactions between risk elements and selected essential macro-and microelements were studied. Rat diet contained 10% of individual soils (based on dry weight): (i) Fluvisol heavily polluted by As, Cd, Zn, and Pb, (ii) Luvisol contaminated by As, Cd, and Zn, and (iii) uncontaminated Chernozem. Male and female Wistar rats used for the experiment were housed in cages in a room with controlled temperature for 60 days and were fed ad libitum the mentioned diets. Subsequently, the pregnant females were continuously fed the experimental diet until weaning when the young animals were separated to male and female and fed the experimental diet till day 110 of age. The element contents in rat tissues reflected the risk element contents in contaminated soils. The bioaccessibility and bioavailability of the risk elements decreased in the order Cd>As>Pb and was affected by the soil physicochemical parameters. No significant differences were observed between male and female rats as well as between the first and the second generation. However, interactions were reported among the risk elements where the high cadmium content in Fluvisol resulted in increasing arsenic accumulation in the rat liver. Moreover, arsenic-copper interactions were observed where significant increase of the copper level was determined in kidney of the animals fed Luvisol exceeding 50-fold the maximum permissible limits for As content in agricultural soils. Among the hematological and biochemical characteristics of rats, total erythrocyte count (Er), hematocrit (Hct) increased confirming adverse effect of soil-derived risk elements especially in male rats.

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Section: Introductionmentioning

confidence: 63%

Long-term effect of diet amended by risk elements contaminated soils on risk element penetration and physiological parameters of rats

Vlčková¹,

Malinová²,

Koubková³

et al. 2014

CZECH J ANIM SCI

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“…These findings need to be proved by the direct determination of metallothionein levels in the tissues in further research. Prigge et al (2002) reviewed the effect of Cd on hematological parameters of animals and mentioned significant decrease of hemoglobin values if the rat diet contained 31 mg/kg of Cd. However, lower levels of iron in the exposed rat organisms were reported in this case.…”

Section: Discussionmentioning

confidence: 99%

Effect of the diet amended with risk elements contaminated soil on risk elements content in tissues and hematological parameters of rats

Száková¹,

Novosadová²,

Zidek³

et al. 2012

CZECH J ANIM SCI

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Penetration of rat organisms by risk elements (As, Cd, Pb) originated from differently contaminated soils as well as interactions between the risk elements and selected essential macro-and microelements were investigated. Rat diet contained 10% of individual soils (based on dry weight) which were: (i) Fluvisol heavily polluted by As, Cd, Zn, and Pb, (ii) Luvisol contaminated by As, Cd, and Zn, and (iii) uncontaminated Chernozem. Male Wistar rats used for the experiment were housed in cages in a room with controlled temperature for 60 days and fed ad libitum on the mentioned diet. The levels of both risk and essential elements were measured in liver, kidney, and bones of the animals and main biochemical parameters were determined to assess potential toxic effect of the soil-derived risk elements. Alternatively, in vitro Physiologically Based Bioavailability Test (PBET) was used for evaluation of the bioaccessible pool of elements in the soil-amended diets. The element contents in the rat tissues reflected the risk element contents in the contaminated soils. Bioaccessibility and bioavailability of the risk elements decreased in the order Cd > As > Pb and were influenced by the soil physicochemical parameters. Significant changes in essential elements (Cu, Fe, Mg, P) deposition in rat tissues attended the high levels of the risk elements. Total leukocyte count in the blood of the rats fed the heavy polluted soil indicated adverse effect of soil-derived risk elements on biochemical parameters of the animals.

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“…For example, the solubility of the hemolysate at 37 "C is considerably lower than the intraerythrocytic concentration of hemoglobins (i.e. about 16 % w/w which corresponds to [Hb] = 20 mM heme for an hematocrit of about 50% [12]); moreover breakage of the red cells shows the presence of microcrystalline material (Fig. 2).…”

Section: Discussionmentioning

confidence: 99%

Hemoglobins from Wistar Rat:

Brunori¹,

Condò²,

Bellelli³

et al. 1982

European Journal of Biochemistry

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The crystallization properties of the hemolysate and of the purified hemoglobin components from Wistar rat have been studied under various experimental conditions. The results suggest the existence of hemoglobins in the crystalline state even within the red blood cells. However the functional properties of rat erythrocytes are identical to those of whole hemolysate in solution.We have recently begun [I] a detailed characterization of the hemoglobin system from Wistar rat, which displays some interesting and peculiar features. The unusual ease and rapidity with which hemoglobin components from Wistar rat crystallize after lysis of erythrocytes is known [2]. The full reversibility of the phenomenon seems to offer an almost unique opportunity to investigate the possible existence of a control mechanism based on ligand-linked polyphasic changes [3]. A quantitative characterization of this phenomenon and its possible physiological significance demands a detailed study (a) of the structural and functional properties of the main hemoglobin components and (b) of the physico-chemical characteristics of the crystallization process, which is obviously of general importance and relevant to the ligand-linked phase changes in hemoglobins, such as HbS.The first point has been the object of a recent paper [l], while the second is the subject of the present investigation. MATERIALS AND METHODSBlood was obtained from anaesthetized Wistar rats (Rattus norvegicus) by cardiac puncture employing a syringe wetted with a solution of heparin (0.1 %) in 0.9 % NaC1. After washing and lysing the cells, the hemoglobin components of the hemolysate were separated as described previously [l 1.Concentrated stock solutions of ~-glycerate-2,3-P, were prepared by dissolving the pentacyclohexylammonium salt (Sigma) in water, converting the salt to the free acid with Dowex 50 and neutralising the solution with NaOH.Inorganic and total acid-soluble P was determined by the method of Chen et al. [4] with minor modifications.Total concentration of ~-glycerate-2,3-P, was determined using the enzymatic Boehringer (Mannheim) ultraviolet test. Relative proportions of hemoglobin components were de- termined by densitometric analysis performed on polyacrylamide gels. Solubility measurements were carried out by centrifuging the suspensions at 8000 rev./min for 15 min at the experimental temperature and determining spectrophotometrically the hemoglobin concentration of the supernatant. Deoxygenation of samples was achieved by gently shaking the samples under a flow of pure nitrogen. Oxygen dissociation curves were determined spectrophotometrically both by the tonometric [5] and thin-layer dilution method [6].Spectrophotometric measurements were carried out with a Cary 219 spectrophotometer.Rat red cells and hemoglobin crystals were examined using a Karl Zeiss microscope Ultraphot 111. The solubility of the hemolysate has been measured under a number of experimental conditions, and the effect of ligation state has also been investigated. RESULTS SolubilityThe ef...

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Effects of dietary and inhalative cadmium on hemoglobin and hematocrit in rats

Cited by 21 publications

References 7 publications

Long-term effect of diet amended by risk elements contaminated soils on risk element penetration and physiological parameters of rats

Long-term effect of diet amended by risk elements contaminated soils on risk element penetration and physiological parameters of rats

Effect of the diet amended with risk elements contaminated soil on risk elements content in tissues and hematological parameters of rats

Hemoglobins from Wistar Rat:

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