Effects of diesel exhaust particles on the health and survival of the buff-tailed bumblebee Bombus terrestris after acute and chronic oral exposure

Hüftlein, Frederic; Seidenath, Dimitri; Mittereder, Andreas; Hillenbrand, Thomas; Brüggemann, Dieter; Otti, Oliver; Feldhaar, Heike; Laforsch, Christian; Schott, M.

doi:10.1016/j.jhazmat.2023.131905

Cited by 7 publications

(6 citation statements)

References 67 publications

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“…While reports exist of contaminated bees in the wild (Negri et al 2015, Thimmegowda et al 2020), we still lack realistic modelling on the uptake of these particles. Other experiments on effects of DEP on individual workers showed increased mortality in B. terrestris workers after oral DEP exposure via sugar water with concentrations of 1 g/l and more(Hüftlein et al 2023). In contrast, we did not see any difference in mortality or other life-history parameters.…”

contrasting

confidence: 95%

“…We randomly selected ten workers per colony and measured the radial cell length of the right forewing under a light microscope (Leica DFC290, Leica M165, Leica, Wetzlar, Germany) to determine worker size (Medler 1962). Additionally, we measured the fat body content of the selected workers according to Hüftlein et al (2023). For that purpose, we separated the abdomen from head and thorax and slightly opened the sternites with tiny scissors.…”

Section: Fat Body Assaymentioning

confidence: 99%

“…In both cases, potential negative effects at such an early stage may lead to a signi cant reduction of the long-term colony tness, such as a lower number of brood and workers, and a higher risk of colony failure (Baron et al 2017). Chronic exposure to high doses of DEPs in sugar water, as a surrogate for plant nectar, increases the mortality of workers of the bumblebee Bombus terrestris, and sublethal doses cause changes in their transcriptome and gut microbiome (Hüftlein et al 2023). However, the impact such effects have on the colony level, including the brood, is still unknown.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the small particle size, APM, such as diesel exhaust particles (DEPs), may enter insects' bodies via tracheae or by consuming contaminated food, such as pollen, which contains APM at least in industrial areas (Feldhaar & Otti 2020, Papa et al 2021. Chemically, DEPs are composed of a carbon core with different organic and inorganic substances adsorbed to its surface, including polycyclic aromatic hydrocarbons (PAHs) (Hüftlein et al 2023, Wichmann 2007, which are known to be harmful to various life forms (Patel et al 2020, Sun et al 2021.…”

Section: Introductionmentioning

confidence: 99%

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Do diesel exhaust particles in pollen affect colony founding in the bumblebee B. terrestris?

Seidenath,

Mittereder,

Hillenbrand

et al. 2024

Preprint

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The global insect decline threatens ecosystem functioning because insects provide many essential services, such as pollination or nutrient cycling. Pollution is one of the main drivers of insect decline besides land-use change, global warming, and invasive species. Airborne particulate matter, such as diesel exhaust particles (DEPs), is ubiquitous in the environment. Their effects on insects are still largely unknown. We provided queens and workers of the bumblebee Bombus terrestris with DEP-spiked pollen at the early colony founding stage and compared the colony development to control colonies. After ten weeks, we investigated individual and colony-level life history traits. We did not see any effects of DEP-spiked pollen on worker count, brood count, worker size and relative fat body weight. These results indicate no harmful effects of DEP on colony founding in B. terrestris in our single-stressor setup. Our novel approach adds to the understanding of the role that airborne particulate matter plays in the global insect decline and we are looking forward to seeing similar studies with other species and additional stressors, such as heat stress or food shortages.

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contrasting

confidence: 95%

Section: Fat Body Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Do diesel exhaust particles in pollen affect colony founding in the bumblebee B. terrestris?

Seidenath,

Mittereder,

Hillenbrand

et al. 2024

Preprint

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“…Animal husbandry was performed as previously described in Hüftlein et al (2023). In brief, we maintained five queenright colonies of the buff-tailed bumblebee Bombus terrestris (Linnaeus, 1758) (Biobest Group NV, Westerlo, Belgium) in a climate chamber under constant conditions at 26℃, 70% humidity, and a 12 h/12 h light/dark cycle.…”

Section: Animal Husbandry and Preparation Of Proventriculimentioning

confidence: 99%

Sexual dimorphism in the proventriculus of the buff‐tailed bumblebee Bombus terrestris (L. 1758) (Hymenoptera: Apidae)

Hüftlein,

Ritschar,

Laforsch

2023

Journal of Morphology

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Research on eusocial bee species like Bombus terrestris is primarily focused on the worker caste, which is why their morphology and anatomy are already well described. This includes the alimentary tract, which is adapted for feeding on nectar and pollen. Located at the transition between crop and ventriculus is a highly specialised compartment, the proventriculus. In female workers of B. terrestris, the proventriculus is surrounded by muscles and consists of four anterior lips. A detailed description, however, is only provided for B. terrestis worker bees while studies on the proventriculus of the male reproductive caste are absent. Here, we provide a detailed analysis of the differences between the proventriculus of the B. terrestris males and females through morphometrics, histology and scanning electron microscopy imaging, and unravel a distinct sexual dimorphism. The male proventriculus is wider resulting in a greater volume than the female proventriculus. Histological analysis revealed 4 distinctive chambers of the male proventriculus, which are completely covered with hairs on the inside. In contrast, those chambers in the proventriculus of female B. terrestris, are only rudimentarily present forming only small pouches with hairs in the junctions between the proventricular folds inside the proventriculus. The morphological differences in the proventriculus may be based on different modi vivendi, as males do not return to the colony and fly longer distances. This and the synthesis of sperm and mating plug might require higher energy reserves, leading to the necessity of higher food storage capacities.

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Do diesel exhaust particles in pollen affect colony founding in the bumble bee Bombus terrestris?

Seidenath,

Mittereder,

Hillenbrand

et al. 2024

Insect. Soc.

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Effects of diesel exhaust particles on the health and survival of the buff-tailed bumblebee Bombus terrestris after acute and chronic oral exposure

Cited by 7 publications

References 67 publications

Do diesel exhaust particles in pollen affect colony founding in the bumblebee B. terrestris?

Do diesel exhaust particles in pollen affect colony founding in the bumblebee B. terrestris?

Sexual dimorphism in the proventriculus of the buff‐tailed bumblebee Bombus terrestris (L. 1758) (Hymenoptera: Apidae)

Do diesel exhaust particles in pollen affect colony founding in the bumble bee Bombus terrestris?

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