Effects of diazinon on the ovarian micro-anatomical and micrometric parameters of pregnant mice

Abstract

Pregnant mice (10 in 4 groups each) were exposed to diazinon (DZN) at 10 and 20 mg/kg on gestation day 6 (GD 6), 9 and 12. A group of 10 pregnant animals was maintained as untreated control. The ovaries were exteriorized on GD 18 by euthanizing the animals. The tissues were processed for histopathological and micrometric analysis. Oocytic nuclear derangements in terms of size increase followed by leakage of nuclear contents with increased duration of exposure were seen. Follicular and luteal cells also showed necrosis. The morphometric data showed significant increase in oocyte and para-follicular size (P_0.0001) along with similar increase in nuclear sizes (P_0.001). These findings indicate that DZN is toxic to bring about histopathological and micrometric derangements in the ovaries of the treated pregnant females.

Ameliorative Activities of Morus and Jamun against Cr Induced Andro-Hepatic Anomalies

“…LDL cholesterol and excess lipid with rise in HDL and VLDL damage liver and cause hepatic steatosis and displace the nucleus towards periphery. Histological studies of liver at micrometric level indicate the alteration central vein CSA in Cr treated group, due to accumulation of debris by [64,74]. Such changes also specify hypothyroidism, excess glucocorticoid and cortisol due to heavy metals exposure [65].…”

“…Natural fruit extracts are without any side effect and never alter the normal cecal microbial composition and intestinal microbiota; essential for carnitine palmitoyl transferase-1 pathway during amelioration [68,72]. The traditional herbal medicines are preferred than modern synthetic drugs and chimeric antigen receptor T-cell therapy against cancer [73,74] along with phytochemicals supplementations will ensure amelioration without side effects in future should be encouraged and sponsored.…”

“…The animals were euthanized by cervical dislocation and dissected out on day 16th for histological hematoxylin and eosin (HE) staining, testicular smear formation and micrometric analysis following [74] protocol. Photograph at 100×, 400× and 1000× with 7.2 MP digital camera (Sony) were processed in CorelDRAW11 for micrometry.…”

“…Comprehensive summary of Ameliorative role of phytochemicals against Cr inducedAndro-hepatic anomalies in mice necrosis. The significant reduction in the CSA of central veins (CV) and number of hepatocytes while the elevation of hepatocytes nuclei size and sinusoidal spaces (SS) clearly intimate liver necrosis[64,74]. Such changes also specify hypothyroidism, excess glucocorticoid and cortisol due to heavy metals exposure[65].…”