Effects of dermal sub-chronic exposure of pubescent male rats to permethrin (PRMT) on the histological structures of genital tract, testosterone and lipoperoxidation

Chargui, Issam; Haouas, Zohra; Zaouali, Monia; Hassen, Ben Cheikh

doi:10.1016/j.etp.2010.02.016

Cited by 33 publications

(19 citation statements)

References 26 publications

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“…Because, the PM administration (group C) caused increases of TOS level and decrease of TAC level as compared to control group (group A). In accordance with our findings, recent investigations clearly indicated that PM-induced oxidative stress leads to biochemical and functional changes in organisms (Nasuti et al 2007;Gabbianelli et al 2009;Issam et al 2011). The present study also demonstrated that the reduction of PM induced oxidative and DNA damages was caused by the protective effect of OLE.…”

Section: Discussionsupporting

confidence: 93%

Olive leaf extract modulates permethrin induced genetic and oxidative damage in rats

2012

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Permethrin is a common synthetic chemical, widely used as an insecticide in agriculture and other domestic applications. The previous reports indicated that permethrin is a highly toxic synthetic pyrethroid pesticide to human and environmental health. Therefore, the present experiment was undertaken to determine the effectiveness of olive leaf extract in modulating the permethrin induced genotoxic and oxidative damage in rats. The animals used were broadly divided into four (A, B, C and D) experimental groups. Group A rats served as control animals and received distilled water intraperitoneally (n = 5). Groups B and C rats received intraperitoneal injections of permethrin (60 mg kg -1 b.w) and olive leaf extract (500 mg kg -1 b.w), respectively. Group D rats received permethrin (60 mg kg -1 b.w) plus olive leaf extract (500 mg kg -1 b.w). Rats were orally administered their respective feed daily for 21 days. At the end of the experiment rats were anesthetized and serum and bone marrow cell samples were obtained. Genotoxic damage was assessed by micronucleus and chromosomal aberration assays. Total antioxidant capacity and total oxidant status were also measured in serum samples to assess oxidative status. Treatment of Group B with permethrin resulted in genotoxic damage and increased total oxidant status levels. Permethrin treatment also significantly decreased (P \ 0.05) total antioxidant capacity level when compared to Group A rats. Group C rats showed significant increases (P \ 0.05) in total antioxidant capacity level and no alterations in cytogenetic parameters. Moreover, simultaneous treatments with olive leaf extract significantly modulated the toxic effects of permethrin in Group D rats. It can be concluded that olive leaf extract has beneficial influences and could be able to antagonize permethrin toxicity. As a result, this investigation clearly revealed the protective role of olive leaf extract against the genetic and oxidative damage by permethrin in vivo for the first time.

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Section: Discussionsupporting

confidence: 93%

Olive leaf extract modulates permethrin induced genetic and oxidative damage in rats

2012

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“…Because, increases in TOS levels and decreases in TAC levels were determined in PM alone treated cultures as compared to control group. In accordance with our findings, recent investigations clearly indicated that PM-induced oxidative stress leads to biochemical and functional changes in organisms (Hu et al, 2010;Nasuti et al, 2007;Gabbianelli et al, 2009;Issam et al, 2011).…”

Section: Discussionsupporting

confidence: 81%

Olive (Olea europaea L.) leaf extract counteracts genotoxicity and oxidative stress of permethrin in human lymphocytes

Türkez

Toğar

2011

J. Toxicol. Sci.

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-The aim of this study was to investigate the protective effects of olive leaf extract (OLE) on genotoxicity and oxidative damage in cultured human blood cells treated with permethrin (PM) in the presence of a rat liver S9 mix containing cytochrome P 450 enzymes. Anti-genotoxic activities of OLE were studied using sister chromatid exchange (SCE) and chromosome aberration (CA) tests and furthermore total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine the oxidative damage. Our results clearly revealed that treatment with PM (200 mg/l) alone increased SCE and CA rates and TOS level, decreased TAC level in cultured human blood cells. The OLE alone at the all tested doses did not induce any significant changes in the genotoxicity endpoint. However OLE leads to increases of plasma TAC level in vitro. OLE starts showing this positive effect at 100 mg/l. The combined treatment showed significant improvements in cytogenetic and biochemical parameters tested. Moreover, this improvement was more pronounced in the group received the high dose of the OLE. It could be concluded that the ethanol extract of OLE induced its genoprotective effect via the increase in the antioxidant capacity, inhibition of oxidative stress and scavenging of free radicals.

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“…Both Perry et al [25] and Yuan et al [26] have observed that increased exposure to permethrin and its metabolites result in lower sperm concentrations in mammals. Present findings are in consonance with, Issam and coworkers [27] who have described that permethrin treated groups have decreased the population of spermatocytes, spermatids and spermatozoa.…”

Section: Discussionsupporting

confidence: 79%

Ameliorative Effects of Phytoandrogen and Antioxidant on Histoarchitecture of Permethrin Induced Changes in Goat Testis

Bhardwaj

Sharma

2017

Int J Pharm Pharm Sci

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Objective: Protective effects of Tribulus terrestris (TT) and vitamin C were studied against permethrin induced changes in the histoarchitecture of goat testis.Methods: Hydroalcoholic extract of the fruits of Tribulus terrestris was prepared by maceration method and the extractive value was calculated. Fresh stock solution of permethrin (1mg/ml) in dimethyl sulphoxide and Tribulus terrestris (1mg/ml) in distilled water were prepared. Testicular tissues were cultured in TCM-199 nutrition media at 39℃ with 95% humidity and 5% CO₂ in the CO₂ incubator. Cultured testicular tissue was grouped as control having only the nutritive media, experimental group I(EI) received two doses of permethrin (100µg/ml and 200µg/ml), experimental group II(EII) was administered doses of permethrin along with vitamin C(0.1µM/ml) and experimental group III(EIII) was supplemented with doses of permethrin along with Tribulus terrestris (100µg/ml). All the groups were tested for 4 hr. and 8hr. for histoarcitectural studies.Results: Histological alterations were observed such as distorted shape of seminiferous tubules, decreased luminal and interstitial space, reduction in the population of spermatocytes, spermatozoa and spermatids in treated (E1) group as compared to control group. Maximum damage was detected at a higher dose of permethrin (200µg/ml) for 8h in contrast to lower doses (100 4h and 8h). Thus, it is concluded that permethrin has both dose and timedependent effects. Protective groups (E2 and E3) imparted ameliorative effects by reestablishing shape of seminiferous tubules, decreasing luminal and interstitial space, increased population of spermatocyte, spermatozoa, and spermatids as compared with treated groups (E1) Conclusion: It has been reported that permethrin affects male fertility by inducing detrimental changes in the structure and function of seminiferous tubules. Both vitamin C and TT have the ability to mitigate the effect of pesticide. TT shows aphrodisiac activity against permethrin induced alterations in goat testis.

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Effects of dermal sub-chronic exposure of pubescent male rats to permethrin (PRMT) on the histological structures of genital tract, testosterone and lipoperoxidation

Cited by 33 publications

References 26 publications

Olive leaf extract modulates permethrin induced genetic and oxidative damage in rats

Olive leaf extract modulates permethrin induced genetic and oxidative damage in rats

Olive (Olea europaea L.) leaf extract counteracts genotoxicity and oxidative stress of permethrin in human lymphocytes

Ameliorative Effects of Phytoandrogen and Antioxidant on Histoarchitecture of Permethrin Induced Changes in Goat Testis

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