Effects of denture adhesives on growth and morphological transformation of Candida albicans

Nomura, Takayoshi; Murakami, Tomohiko; Shimoyama, Yu; Kobayashi, Takuya; Furuya, Junichi; Sasaki, Masahiro; Kondo, Hisatomo

doi:10.1016/j.jpor.2019.04.006

Cited by 5 publications

(4 citation statements)

References 34 publications

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“…Those were extracted from S. salivarius ATCC 7073 (1.0 × 10 2 to 1.0 × 10 6 CFU) and C. albicans SC 5314 (1.0 × 10 1 to 1.0 × 10 6 CFU) cells. The numbers of S. salivarius and C. albicans were determined by plating culture dilutions in trypticase soy agar and YPD agar plates, respectively [38,40]. The linearity of these assays was correlated between the Ct and microbial amounts.…”

Section: Determination Of Microbial Amountsmentioning

confidence: 99%

Associations of streptococci and fungi amounts in the oral cavity with nutritional and oral health status in institutionalized elders: a cross sectional study

et al. 2021

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Background Disruption of the indigenous microbiota is likely related to frailty caused by undernutrition. However, the relationship between undernutrition and the oral microbiota, especially normal bacteria, is not obvious. The aim of this study was to elucidate the associations of nutritional and oral health conditions with prevalence of bacteria and fungi in the oral cavity of older individuals. Methods Forty-one institutionalized older individuals with an average age ± standard deviation of 84.6 ± 8.3 years were enrolled as participants. Body mass index (BMI) and oral health assessment tool (OHAT) scores were used to represent nutritional and oral health status. Amounts of total bacteria, streptococci, and fungi in oral specimens collected from the tongue dorsum were determined by quantitative polymerase chain reaction (PCR) assay results. This study followed the STROBE statement for reports of observational studies. Results There was a significant correlation between BMI and streptococcal amount (ρ = 0.526, p < 0.001). The undernutrition group (BMI < 20) showed a significantly lower average number of oral streptococci (p = 0.003). In logistic regression models, streptococcal amount was a significant variable accounting for “not undernutrition” [odds ratio 5.68, 95% confidential interval (CI) 1.64–19.7 (p = 0.06)]. On the other hand, participants with a poor oral health condition (OHAT ≥ 5) harbored significantly higher levels of fungi (p = 0.028). Conclusion Oral streptococci were found to be associated with systemic nutritional condition and oral fungi with oral health condition. Thus, in order to understand the relationship of frailty with the oral microbiota in older individuals, it is necessary to examine oral indigenous bacteria as well as etiological microorganisms.

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Section: Determination Of Microbial Amountsmentioning

confidence: 99%

Associations of streptococci and fungi amounts in the oral cavity with nutritional and oral health status in institutionalized elders: a cross sectional study

et al. 2021

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“…Those were extracted from S. salivarius ATCC 7073 (1.0 x 10 2 to 1.0 x 10 6 CFU) and C. albicans SC 5314 (1.0 x 10 1 to 1.0 x 10 6 CFU) cells. The numbers of S. salivarius and C. albicans were determined by plating culture dilutions in trypticase soy agar and YPD agar plates, respectively [35,37]. The linearity of these assays was correlated between the Ct and microbial amounts.…”

Section: Determination Of Microbial Amountsmentioning

confidence: 99%

Associations of streptococci and fungi amounts in the oral cavity with nutritional and oral health status in institutionalized elders: a cross sectional study

Sato

Yano

Shimoyama

et al. 2021

Preprint

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Background:Disruption of the indigenous microflora is likely to relate with frailty caused by undernutrition. However, relationship between undernutrition and oral flora, especially normal indigenous bacteria, is not obvious. The aim of this study was to elucidate the associations of nutritional and oral health conditions with bacteria and fungi prevalence in oral cavity of older individuals.Methods:Forty-one institutionalized older individuals with an average age ± standard deviation of 84.6 ± 8.3 years were enrolled in this study. Body mass index (BMI) and Oral health assessment tool (OHAT) were used to represent nutritional and oral health status. Amounts of total bacteria, streptococci, and fungi in oral specimens collected from the tongue dorsum were determined by quantitative polymerase chain reaction (PCR) assay results. Results:There found a significant correlation between BMI and streptococcal amount (ρ=0.526, p<0.001). In addition, the undernutrition group (BMI <20) showed a significantly lower average number of oral streptococci (p=0.003). In logistic regression models, streptococcal amount was the significant variable accounting for “not undernutrition” [odds ratio 5.68 and 95% confidential interval (CI) was 1.64-19.7 (p=0.06)]. On the other hand, participants with poor oral health condition (OHAT ≥5) harbored significantly higher level of fungi (p=0.028). Conclusion:Oral streptococci were associated with systemic nutritional condition and oral fungi were associated with oral health condition. Thus, it is necessary to examine oral indigenous bacteria as well as etiological microorganisms in order to understand the relationship of frailty with oral microflora in older individuals.

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“…The adhesive substances include karaya gum, gelatin, pectin, methylcellulose, hydroxymethyl cellulose, and carboxymethyl cellulose sodium salt 3 . The main purpose of the antimicrobials, such as sodium borate, sodium tetraborate, hexachlorophene or propyl hydroxybenzoate, is to prevent the appearance of Candida Albicans fungal infections because some studies have shown that the use of denture adhesives increased its growth 3,7 . Finally, the additives that serve as fillers, such as magnesium oxide, in addition to moistening, are intended to impart a pleasant taste for the user (e.g., mint or gaultheria oil) 8,9 …”

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Are Denture Adhesives Safe for Oral Cells?

López‐García

Pecci-Lloret

García‐Bernal

et al. 2020

Journal of Prosthodontics

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Purpose To compare the cytotoxicity of six commercially available denture adhesives on human gingival cells: Poligrip Flavour Free Fixative Cream, Fixodent Pro Duo Protection, Novafix cream, FittyDent, Polident Total Action, and Fixodent Pro Plus Duo Protection. Material and Methods Eluates of denture adhesives were brought into contact with human gingival cells and compared to untreated cells (w/o any dental adhesive elute). Cell toxicity was assessed by measuring cell viability (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) (MTT) assays), cell morphology (immunofluorescence assays), induction of apoptosis/necrosis and production of reactive oxygen species (ROS) (flow cytometry assays). In addition, the pH of each sample was determined. Data were analyzed using one‐way analysis of variance (ANOVA) followed by Dunnett's multiple comparisons test. Results All denture adhesives tested led to a reduction in pH, especially Fixodent Pro Duo Protection and Fixodent Pro Plus Duo Protection. The cell viability assays showed that Fixodent Pro Duo Protection (1:1 72 hours, p = 3.04 × 10−6; 1:2 72 hours, p = 2.07 × 10−6; 1:4 72 hours, p = 2.04 × 10−6) and Fixodent Pro Plus Duo Protection (1:1 72 hours, p = 2.01 × 10−6; 1:2 72 hours, p = 3.03 × 10−6; 1:4 72 hours, p = 2.02 × 10−6) significantly decreased cell viability at all dilutions. Compared to the control group and the rest of the adhesives, Poligrip Flavour Free Fixative Cream (PFF 1:1 72 hours, p = 2.24 × 10−6; 1:2 72 hours, p = 2.44 × 10−6; 1:4 72 hours, p = 2.04 × 10−6) showed a significantly higher cell viability score at all dilutions. Fixodent Pro Duo Protection and Fixodent Pro Plus Duo Protection, both adhesives containing zinc salts in their composition, were responsible for necrosis, and the number of cells was much reduced, with aberrant morphology and pyknotic nucleus. Finally, Fixodent (1:2, p = 2.04 × 10−6, 1:4, p = 0.00036; 1:2, p = 8.82 × 10−6, 1:4, p = 2.30 × 10−6) products significantly promoted ROS production in gingival cells. Conclusions The results suggest that denture adhesives containing zinc in their composition could be responsible of the decrease of cell viability, ROS production, aberrant cell morphology, and induction of apoptosis and cell death. However, other possible additional cytotoxic factors must be considered. Thus, more studies are necessary to confirm this hypothesis.

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Effects of denture adhesives on growth and morphological transformation of Candida albicans

Cited by 5 publications

References 34 publications

Associations of streptococci and fungi amounts in the oral cavity with nutritional and oral health status in institutionalized elders: a cross sectional study

Associations of streptococci and fungi amounts in the oral cavity with nutritional and oral health status in institutionalized elders: a cross sectional study

Associations of streptococci and fungi amounts in the oral cavity with nutritional and oral health status in institutionalized elders: a cross sectional study

Are Denture Adhesives Safe for Oral Cells?

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