Effects of denervation at ileocecal junction and ileocecal resection in dogs

Morita, Hiroki; Mochiki, Erito; Ogawa, Atsushi; Yanai, Mitsuhiro; Toyomasu, Yoshitaka; Tabe, Yuichi; Ohno, Tetsuro; Tsutsumi, Souichi; Asao, Takayuki; Kuwano, Hiroyuki

doi:10.1111/j.1365-2982.2011.01810.x

Cited by 7 publications

(3 citation statements)

References 38 publications

(89 reference statements)

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“…Colonic migrating motor complexes (CMMCs) are one of the major types of motor pattern that occur in the large intestine of a variety of mammals both in vivo (Ferre and Ruckebusch, 1985; Sarna, 1991; Morita et al, 2012) and in vitro (Wood, 1973; Fida et al, 1997; Bywater et al, 1998; Brierley et al, 2001; Powell and Bywater, 2001; Roberts et al, 2007, 2008; Heredia et al, 2009; Copel et al, 2013). CMMCs have also been reported in human colon in vivo (Hagger et al, 2003) and recently in the intact whole isolated human colon (Spencer et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Characteristics of colonic migrating motor complexes in neuronal NOS (nNOS) knockout mice

Spencer

2013

Front. Neurosci.

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It is well established that the intrinsic pacemaker mechanism that generates cyclical colonic migrating motor complexes (CMMCs) does not require endogenous nitric oxide (NO). However, pharmacological blockade of endogenous NO production potently increases the frequency of CMMCs, suggesting that endogenous NO acts normally to inhibit the CMMC pacemaker mechanism. In this study, we investigated whether mice with a life long genetic deletion of the neuronal nitric oxide synthase (nNOS) gene would show similar CMMC characteristics as wild type mice that have endogenous NO production acutely inhibited. Intracellular electrophysiological and mechanical recordings were made from circular muscle cells of isolated whole mouse colon in wild type and nNOS knockout (KO) mice at 35°C. In wild type mice, the NOS inhibitor, L-NA (100 μM) caused a significant increase in CMMC frequency and a significant depolarization of the CM layer. However, unexpectedly, the frequency of CMMCs in nNOS KO mice was not significantly different from control mice. Also, the resting membrane potential of CM cells in nNOS KO mice was not depolarized compared to controls; and the amplitude of the slow depolarization phase underlying MCs was of similar amplitude between KO and wild type offspring. These findings show that in nNOS KO mice, the major characteristics of CMMCs and their electrical correlates are, at least in adult mice, indistinguishable from wild type control offspring. One possibility why the major characteristics of CMMCs were no different between both types of mice is that nNOS KO mice may compensate for their life long deletion of the nNOS gene, and their permanent loss of neuronal NO production. In this regard, we suggest caution should be exercised when assuming that data obtained from adult nNOS KO mice can be directly extrapolated to wild type mice, that have been acutely exposed to an inhibitor of NOS.

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Section: Introductionmentioning

confidence: 99%

Characteristics of colonic migrating motor complexes in neuronal NOS (nNOS) knockout mice

Spencer

2013

Front. Neurosci.

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“…The intrinsic denervation distal to the bowel resection is a crucial factor. In a study performed on dogs, Morita et al 21 found an increased rate of colonic migrating motor complexes and a reduced rate of colonic nonmigrating motoric complexes following intrinsic denervation of the colon. They found a clear relation between intrinsic denervation of the colon and diarrhea.…”

Section: Discussionmentioning

confidence: 98%

Bowel Motility After Injury to the Superior Mesenteric Plexus During D3 Extended Mesenterectomy

Thorsen

Štimec

Lindstrøm

et al. 2019

Journal of Surgical Research

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Background: Improvement of lymphadenectomy in right colectomy requires removal of all tissue surrounding the superior mesenteric vessels beneath the pancreatic notch. Shortand long-term bowel motility disorders after D3 extended mesenterectomy with consecutive superior mesenteric plexus transection are studied. Methods: Patients without pre-existing motility disorders undergoing D3 extended mesenterectomy were examined 3 times using the wireless motility capsule: before, at 3 wk, and 6 mo after surgery. Segmental transit times and contractility were analyzed using mixed effect modeling. Correlation between contractility and transit time was assessed by the Pearson correlation coefficient. Results: Fifteen patients (4 men), with median age 62 y, were included. Mean values for the three consecutive examinations are as follows. Gastric transit time increased from 237 to 402 and 403 min, respectively. Small bowel transit time decreased from 246 to 158 (P < 0.01) and 199 (P ¼ 0.03) min, respectively. Colonic transit time decreased from 1742 to 1450 and 1110 (P ¼ 0.02) min, respectively. Gastric contractions per minute (CPM) varied from 1.73 to 1.05 (P ¼ 0.01) and 2.47 (P < 0.01), respectively. Small bowel CPM decreased from 3.43 to 2.68 and 3.34, respectively. Colonic CPM ranged from 1.59 to 1.45 and 1.91 (P ¼ 0.08), respectively. Correlation between small bowel (SB) transit time and CPM was À0.45 (P ¼ 0.09) preoperatively, and À0.03 (P ¼ 0.91) 6 mo postoperatively. Conclusions: Extrinsic SB denervation leads to significantly accelerated SB transit, reduced contractility, and disturbed correlation between transit time and contractility early after denervation. Both number of contractions and transit time in the denervated SB show a clear tendency toward normalization at 6 mo.

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“…Thus, we were able to compare the effects of various reagents in the same animals. In this context, four to six dogs have been used in recent studies from our institution (Hayashi et al 2010;Sato et al 2010a) and other institutions (Dong et al 2005;Morita et al 2012;Yanai et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Intragastric Dai-Kenchu-To, a Japanese Herbal Medicine, Stimulates Colonic Motility via Transient Receptor Potential Cation Channel Subfamily V Member 1 in Dogs

Kikuchi

Shibata

Imoto

et al. 2013

Tohoku J. Exp. Med.

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Japanese herbal medicine, also known as Kampo, is used for various diseases in Japan. One of those medicines, Dai-Kenchu-To (DKT), is considered clinically effective for adhesive bowel obstruction and chronic constipation. Although scientific evidence of DKT to improve adhesive bowel obstruction was shown in several previous reports, mechanism of DKT to improve constipation remains unknown. Our aim was to study the effect of intragastric DKT on colonic motility and defecation, and the involvement of various receptors in DKT-induced colonic contractions. Five beagle dogs were instructed with serosal strain-gauge force transducers to measure circular muscle activity at the proximal, middle, and distal colon. Dogs are suitable for a present study to administer the drugs repeatedly to the same individual and look at its effect on colonic motility. We studied the effects of DKT (2.5 or 5 g) administered into the stomach on colonic motility. Muscarinic receptor antagonist atropine, nicotinic receptor antagonist hexamthonium, or 5-hydroxytryptamine-3 receptor antagonist ondansetron was injected intravenously 10 min before DKT administration. Capsazepine, an antagonist to transient receptor potential cation channel subfamily V member 1 (TRPV1), was administered into the stomach 5 min before DKT administration. Intragastric DKT (2.5 or 5 g) induced colonic contractions within 10 min after administration but did not induce defecation. Pretreatment with atropine, hexamthonium, ondansetron, or capsazepine inhibited DKT-induced colonic contractions. These results indicate that orally administered DKT stimulates colonic motility via TRPV1, muscarinic, nicotinic, and 5-hydroxytryptamine-3 receptors, thereby providing scientific support for the efficacy of oral DKT in chronic constipation.

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Effects of denervation at ileocecal junction and ileocecal resection in dogs

Abstract: Gastrocolonic response at the ICJ may require both intrinsic and extrinsic innervation. When ID was performed, CMMC ratio increased. As a result, intraluminal water absorption may have decreased. ID may be one of the causes of diarrhea after ICR.

Cited by 7 publications

References 38 publications

Characteristics of colonic migrating motor complexes in neuronal NOS (nNOS) knockout mice

Characteristics of colonic migrating motor complexes in neuronal NOS (nNOS) knockout mice

Bowel Motility After Injury to the Superior Mesenteric Plexus During D3 Extended Mesenterectomy

Intragastric Dai-Kenchu-To, a Japanese Herbal Medicine, Stimulates Colonic Motility via Transient Receptor Potential Cation Channel Subfamily V Member 1 in Dogs

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