Effects of Dendrobium officinale polysaccharide on adipogenic differentiation of rat bone marrow mesenchymal stem cells

Zhao, Yongmei; Xu, Lujia; Hua, Yingqi

doi:10.1590/1678-457x.22716

Cited by 6 publications

(6 citation statements)

References 20 publications

(18 reference statements)

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“…Subsequently, 90% (v/v) ethanol was used to elute the resin and dissolve the adsorbed flavanone in ethanol. The AJWTF extract was ultimately obtained by steam-drying ethanol [14].…”

Section: Methodsmentioning

confidence: 99%

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Preventive Effect of Anji White Tea Flavonoids on Alcohol-Induced Gastric Injury through Their Antioxidant Effects in Kunming Mice

Liu

Feng

Zhang³

et al. 2019

Biomolecules

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Anji white tea (Camellia sinensis) is a traditional Chinese tea beverage, which is classified as green tea and contains an abundant amount of flavonoids. In this study, the preventive effect of Anji white tea flavonoids (AJWTFs) on ethanol/hydrochloric acid-induced gastric injury in mice was evaluated. The serum and gastric tissues of mice were analyzed using a biochemical kit and by quantitative polymerase chain reaction (qPCR). Observation of the appearance of the stomach indicated that AJWTFs could effectively reduce the area of gastric injury caused by ethanol/hydrochloric acid, and the inhibition rate of AJWTF on gastric injury increased with an increase in AJWTF concentration. The Anji white tea flavonoids could also reduce the volume and pH of gastric juice in mice with gastric injury. Biochemical results showed that AJWTFs could increase the superoxide dismutase (SOD) and glutathione (GSH) activities, as well as decrease the malondialdehyde (MDA) level, in the serum and liver of mice with gastric injury. Pathological observation confirmed that AJWTFs could inhibit the tissue damage caused by ethanol/hydrochloric acid in the stomach of mice. Further qPCR experiments also showed that AJWTFs could inhibit the decreases in neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS), copper/zinc superoxide dismutase (Cu/Zn–SOD), manganese superoxide dismutase (Mn–SOD), catalase (CAT), and the increase in inducible nitric oxide synthase (iNOS) expression in the gastric tissue of mice caused by gastric injury. As observed, AJWTFs exerted a good preventive effect on alcohol-induced gastric injury in mice induced by ethanol/hydrochloric acid, and the effect is close to that of ranitidine. Anji white tea flavonoids present good antioxidant effect, which allows them to effectively prevent alcoholic gastric injury and be used as biologically active substances with a broad range of applications.

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“…Subsequently, 90% (v/v) ethanol was used to elute the resin and dissolve the adsorbed flavanone in ethanol. The AJWTF extract was ultimately obtained by steam-drying ethanol [14].…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, 0.01 g of AJWTF extract was dissolved in 5 mL of 90% ethanol, and 2 mL of AJWTF extract solution was poured into a 25-mL volumetric flask. The flavonoid content in the AJWTF extract was determined using the repeated rutin standard curve method (rutin meter) [14].…”

Section: Methodsmentioning

confidence: 99%

Preventive Effect of Anji White Tea Flavonoids on Alcohol-Induced Gastric Injury through Their Antioxidant Effects in Kunming Mice

Liu

Feng

Zhang³

et al. 2019

Biomolecules

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show abstract

“…23 Moreover, ZHAO et al revealed that DOP has a great role in regulating cell fate of BMSCs. 24 It could inhibit adipogenic differentiation in Rat BMSCs by deregulating adipogenesis-related gene expression of PPARγ, LPL and FABP4. 24 However, the role of DOP in age-related bone loss and lineage commitment of BMSCs is still unclear.…”

mentioning

confidence: 99%

Dendrobium officinale polysaccharides regulate age‐related lineage commitment between osteogenic and adipogenic differentiation

et al. 2019

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Objectives: Excessive oxidative stress and diminished antioxidant defences could contribute to age-related tissue damage and various diseases including age-related osteoporosis. Dendrobium officinale polysaccharides (DOPs), a major ingredient from a traditional Chinese medicine, have a great potential of antioxidative activity. In this study, we explore the role of DOP in age-related osteoporosis that remains elusive. Materials and methods:Oxidative stimulation and DOP were used to treat bone marrow mesenchymal stem cells (BMSCs), whose lineage commitment was measured by adipogenic-and osteoblastic-induced differentiation analysis. The oxidative stress and antioxidant capacity of BMSCs under the treatment of DOP were analysed by the level of MDA, SOD. Related mechanism studies were confirmed by qRT-PCR, Western blotting and siRNA transfection. DOP was orally administrated in aged mice whose phenotype was confirmed by micro-CT, immunofluorescence, immunochemistry and calcein double-labelling analysis.Results: Dendrobium officinale polysaccharide treatment markedly increased osteogenic differentiation of BMSCs, while inhibiting adipogenic differentiation. In vitro, DOP could rescue H2O2-induced switch of BMSCs differentiation fate. However, this effect was abolished in BMSCs when interfered with Nrf2 siRNA. Furthermore, administration of DOP to aged mice significantly increased the bone mass and reduced the marrow adipose tissue (MAT) accompanied with decreased oxidative stress of BMSCs. Conclusions:Our study reveals that DOP can attenuate bone loss and MAT accumulation through NRF2 antioxidant signalling, which may represent as potential therapeutic agent for age-related osteoporosis.

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“…Nowadays, it is popular to extract the biological products from the plants and microorganism, which have been used in the protection of plants or human beings, due to the natural friendliness (Dai et al, 2017;Dong et al, 2014;Zhao et al, 2017). Prodigiosin is a critical bioactive compound with common applications in the food, pharmaceutical, cosmetic and textile industries.…”

Section: Introductionmentioning

confidence: 99%

Structure of prodigiosin from Serratia marcescens NJZT-1 and its cytotoxicity on TSC2-null cells

et al. 2021

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Prodigiosin, a secondary metabolite extracted from Serratia marcescens (S. marcescens), could induce apoptosis in various cancer cells, with however low toxicity on normal cells. The red pigment was extracted from a strain S. marcescens NJZT-1 isolated from soil, which had antibacterial activity. Spectral analyses (LC-ESI-MS, UV-VIS spectrophotometry, infrared spectra and HPLC) and TLC indicated the presence of prodigiosin in the extracellular bacterial culture extracts. The red pigment effectively killed the TSC2-null cells, whose mutation resulted in the progressive and systemic disease LAM. Our findings provide interesting evidence and important basis for the development of new therapeutic compounds with high potential effects against TSC2-null cells.

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Effects of Dendrobium officinale polysaccharide on adipogenic differentiation of rat bone marrow mesenchymal stem cells

Cited by 6 publications

References 20 publications

Preventive Effect of Anji White Tea Flavonoids on Alcohol-Induced Gastric Injury through Their Antioxidant Effects in Kunming Mice

Preventive Effect of Anji White Tea Flavonoids on Alcohol-Induced Gastric Injury through Their Antioxidant Effects in Kunming Mice

Dendrobium officinale polysaccharides regulate age‐related lineage commitment between osteogenic and adipogenic differentiation

Structure of prodigiosin from Serratia marcescens NJZT-1 and its cytotoxicity on TSC2-null cells

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