Effects of delayed administration of (−)-epigallocatechin gallate, a green tea polyphenol on the changes in polyamine levels and neuronal damage after transient forebrain ischemia in gerbils

Lee, So‐Young; Kim, Choong-Young; Lee, Jung-Jeung; Jung, Jung-Gil; Lee, Seong‐Ryong

doi:10.1016/s0361-9230(03)00139-4

Cited by 53 publications

(32 citation statements)

References 48 publications

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“…against neuronal damage, brain edema, and LPO in in vivo and in vitro experiments on gerbil brain ischemia. [13][14][15] ECGC produced a higher NF-L protein expression and higher density of retinal ganglionic cells after optic nerve crush. 8 In addition, EGCG dramatically reduced astrogliosis, as demonstrated by GFAP expression and neuronal loss in an HIV-1 Tat Tg mouse model.…”

Section: Discussionmentioning

confidence: 89%

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(-)-Epigallocatechin-3-Gallate Modulates Spinal Cord Neuronal Degeneration by Enhancing Growth-Associated Protein 43, B-Cell Lymphoma 2, and Decreasing B-Cell Lymphoma 2-Associated X Protein Expression after Sciatic Nerve Crush Injury

Renno

Al‐Maghrebi²,

Rao³

et al. 2015

Journal of Neurotrauma

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Our previous studies have established that (-)-epigallocatechin-3-gallate (EGCG) has both neuroprotective and -regenerative capacity after sciatic nerve injury. Moreover, this improvement was evident on the behavioral level. The aim of this study was to investigate the central effects of ECGC on spinal cord motor neurons after sciatic nerve injury. Our study showed that administering 50 mg/kg intraperitoneally i.p. of EGCG to sciatic nerve-injured rats improved their performance on different motor functions and mechanical hyperesthesia neurobehavioral tests. Histological analysis of spinal cords of EGCG-treated sciatic nerve-injured (CRUSH + ECGC) animals showed an increase in the number of neurons in the anterior horn, when compared to the naïve, sham, and saline-treated sciatic nerve-injured (CRUSH) control groups. Additionally, immunohistochemical study of spinal cord sections revealed that EGCG reduced the expression of glial fibrillary acidic protein and increased the expression of growth-associated protein 43, a marker of regenerating axons. Finally, EGCG reduced the ratio of B-cell lymphoma 2 (Bcl-2)-associated X protein/Bcl-2 and increased the expression of survivin gene. This study may shed some light on the future clinical use of EGCG and its constituents in the treatment of peripheral nerve injury.

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Section: Discussionmentioning

confidence: 89%

“…The recommended dose of EGCG, in many studies, was 50 mg/kg i.p. 4,[13][14][15][16][17] Therefore, we injected 50 mg/kg (i.p.) of EGCG daily for 3 days, starting 1 h after sciatic nerve crush injury in the treated group.…”

Section: Surgical Proceduresmentioning

confidence: 99%

(-)-Epigallocatechin-3-Gallate Modulates Spinal Cord Neuronal Degeneration by Enhancing Growth-Associated Protein 43, B-Cell Lymphoma 2, and Decreasing B-Cell Lymphoma 2-Associated X Protein Expression after Sciatic Nerve Crush Injury

Renno

Al‐Maghrebi²,

Rao³

et al. 2015

Journal of Neurotrauma

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“…Furthermore, the molecular mechanism by which EGCG affects Sema3 signaling requires further study. Interestingly, EGCG has neuroprotective and anti-inflammatory effects, however, the molecular substrates responsible for these effects are poorly understood (Koh et al, 2004;Lee et al, 2003;Levites et al, 2001;Mandel et al, 2004).…”

Section: Egcg Neutralizes Axon Repulsion By Sema3smentioning

confidence: 99%

MICAL flavoprotein monooxygenases: Expression during neural development and following spinal cord injuries in the rat

Pasterkamp

Dai

Terman³

et al. 2006

Molecular and Cellular Neuroscience

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“…One possible explanation for the sporicidal capacity of RESV is that it interferes in the polyamine metabolism pathways that are essential for microsporidian development (1); indeed, polyamine inhibitors have been demonstrated to be effective for the treatment of microporidiosis (2). It is well known that polyphenols provoke changes in polyamine levels in various cell types (21). Specifically, it has been shown that RESV inhibits polyamine synthesis and increases polyamine catabolism (39).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effects of Resveratrol on the Viability and Infectivity of the Microsporidian Encephalitozoon cuniculi

Leiro¹,

Cano

Ubeira³

et al. 2004

Antimicrob Agents Chemother

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Microsporidians of the genus Encephalitozoon are an important cause of disease in immunocompromised patients, and there are currently no completely effective treatments. The present study investigated the viability and infectivity of spores of Encephalitozoon cuniculi that had been exposed to resveratrol (RESV), a natural phytoalexin found in grapes and red wine. RESV at 50 M showed significant sporicidal activity, and at 10 to 50 M it reduced the capacity of the spores to infect dog kidney epithelial cells of the MDCK line. At 10 M RESV also significantly inhibited intracellular development of the parasite, without affecting host cell viability. These results suggest that RESV may be useful in the treatment of Encephalitozoon infections.Microsporidians are ubiquitous small intracellular sporeforming pathogens that infect a wide range of vertebrate and invertebrate species, including humans, and that are an increasingly significant cause of disease in immunocompromised patients (36). To date, more than 1,200 species within 144 genera have been described (24). Species of the genera Nosema, Vittaforma, Brachiola, Pleistophora, Encephalitozoon, Enterocytozoon, Septata (now reclassified as Encephalitozoon), and Trachipleistophora have been found in human infections (38). Encephalitozoon cuniculi is commonly found in domestic rabbits and rodents, and also occurs in dogs and other canids and primates, including humans (32,34,36,40). Currently, no completely effective treatment exists for human microsporidiosis. The most widely used drugs for the treatment of microsporidiosis in animals and humans are albendazole and fumagillin (11,25). However, infections show variable responses to these drugs: albendazole appears to show consistent efficacy against Encephalitozoon but is variable in its efficacy against Enterocytozoon, though fumagillin appears to be effective against both species of Encephalitozoon and Enterocytozoon (7,25). Resveratrol (3,4Ј,5-trihydroxystilbene) (RESV), a natural phytoalexin produced by certain spermatophytes in response to infection by phytopathogenic microorganisms and found in grapes and grape products such as red wine, has known anticancer and anti-inflammatory effects (13,15). One probable function of RESV is to protect the plant against fungal infections (16,29), and some studies indicate that RESV may be useful for combatting human fungal pathogens (5). The microsporidians were initially considered to be protozoa but are currently considered to be parasitic fungi (4,18,23,35). In the present study we evaluated for the first time the effects of RESV on the in vitro viability and infectivity of spores of the microsporidian E. cuniculi and investigated the possible toxicity of this compound to host cells. MATERIALS AND METHODSOrganism, cell culture, media, drugs, and chemicals. A total of 10 7 spores of a canine subtype of E. cuniculi (ATCC 50502) were grown in 75-cm 2 culture flasks (Costar) containing mycoplasma-free epithelial dog kidney cells of the MDCK line (ATCC CCL-34). The cells were ...

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Effects of delayed administration of (−)-epigallocatechin gallate, a green tea polyphenol on the changes in polyamine levels and neuronal damage after transient forebrain ischemia in gerbils

Cited by 53 publications

References 48 publications

(-)-Epigallocatechin-3-Gallate Modulates Spinal Cord Neuronal Degeneration by Enhancing Growth-Associated Protein 43, B-Cell Lymphoma 2, and Decreasing B-Cell Lymphoma 2-Associated X Protein Expression after Sciatic Nerve Crush Injury

(-)-Epigallocatechin-3-Gallate Modulates Spinal Cord Neuronal Degeneration by Enhancing Growth-Associated Protein 43, B-Cell Lymphoma 2, and Decreasing B-Cell Lymphoma 2-Associated X Protein Expression after Sciatic Nerve Crush Injury

MICAL flavoprotein monooxygenases: Expression during neural development and following spinal cord injuries in the rat

In Vitro Effects of Resveratrol on the Viability and Infectivity of the Microsporidian Encephalitozoon cuniculi

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