Effects of degeneration on the compressive and tensile properties of human meniscus

Fischenich, Kristine M.; Lewis, Jackson T.; Kindsfater, Kirk; Bailey, Travis S.; Donahue, Tammy L. Haut

doi:10.1016/j.jbiomech.2015.02.042

Cited by 60 publications

(55 citation statements)

References 26 publications

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“…Wheatley et al (2015) utilized the inverse FE approach to demonstrate that menisci subjected to a traumatic compressive load had decreased material properties at twelve weeks post-impact compared to control. While Fischenich et al (2015) showed that with increasing gross tissue damage, changes in compressive properties, but not in tensile properties, occurred.…”

mentioning

confidence: 94%

Meniscus mechanics and mechanobiology

Donahue

Fisher

Maher

2015

Journal of Biomechanics

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mentioning

confidence: 94%

Meniscus mechanics and mechanobiology

Donahue

Fisher

Maher

2015

Journal of Biomechanics

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“…Reduction of GAG release and expression of matrix degrading enzymes MMP3, MMP13 and ADAMTS4 indicated protective effects of IL-10. Although extracellular matrix in meniscus tissue only consists of 1% GAG, it has been demonstrated to essentially contribute to compressive and tensile properties in human meniscus [31]. Recently, similar findings have been demonstrated in an in vitro model of mechanically injured articular cartilage.…”

Section: Discussionmentioning

confidence: 69%

IL-10 ameliorates TNF-α induced meniscus degeneration in mature meniscal tissue in vitro

Behrendt¹,

Häfelein

Preusse-Prange

et al. 2017

BMC Musculoskelet Disord

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BackgroundJoint inflammation causes meniscus degeneration and can exacerbate post-traumatic meniscus injuries by extracellular matrix degradation, cellular de-differentiation and cell death. The aim of this study was to examine whether anti-inflammatory interleukin-10 exerts protective effects in an in vitro model of TNF-α-induced meniscus degeneration.MethodsMeniscus tissue was harvested from the knees of adult cows. After 24 h of equilibrium explants were simultaneously treated with bovine TNF-α and IL-10. After an incubation time of 72 h cell death was measured histomorphometrically (nuclear blebbing, NB) and release of glycosaminoglycans (GAG, DMMB assay) and nitric oxide (NO, Griess-reagent) were analysed. Transcription levels (mRNA) of matrix degrading enzymes, collagen type X (COL10A1) and nitric oxide synthetase 2 (NOS2) were measured by quantitative real time PCR. TNF-α-dependent formation of the aggrecanase-specific aggrecan neoepitope NITEGE was visualised by immunostaining. Differences between groups were calculated using a one-way ANOVA with a Bonferroni post hoc test.ResultsAdministration of IL-10 significantly prevented the TNF-α-related cell death (P .001), release of NO (P .003) and NOS2 expression (P .04). Release of GAG fragments (P .001), NITEGE formation and expression of MMP3 (P .007), -13 (P .02) and ADAMTS4 (P .001) were significantly reduced. The TNF-α-dependent increase in COL10A1 expression was also antagonized by IL-10 (P .02).ConclusionIL-10 prevented crucial mechanisms of meniscal degeneration induced by a key cytokine of OA, TNF-α. Administration of IL-10 might improve the biological regeneration and provide a treatment approach in degenerative meniscus injuries and in conditions of post-traumatic sports injuries.

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“…The TPE hydrogel only lost 19% of the initial response compared with the human and ovine tissues which experienced a drop of 95 and 82%, respectively. Compressive mechanical deterioration of the menisci has been shown to occur in patients with osteoarthritis and increase with the severity of osteoarthritis . The pathogenesis of the disease and subsequent relationship to the mechanical integrity of the surrounding tissues is still not fully understood.…”

Section: Discussionmentioning

confidence: 99%

“…Compressive mechanical deterioration of the menisci has been shown to occur in patients with osteoarthritis and increase with the severity of osteoarthritis. 51 The pathogenesis of the disease and subsequent relationship to the mechanical integrity of the surrounding tissues is still not fully understood. It may be advantageous to design a replacement material that still exhibits some viscoelasticity but does not relax to the extent of the native tissue.…”

Section: Discussionmentioning

confidence: 99%

“…[3][4][5] A number of studies have investigated the compressive mechanical properties of human menisci under confined 6,7 and unconfined environments [8][9][10][11][12][13] as well as indentation relaxation. 11,12,14 Little investigation of the dynamic properties of the tissue has been performed, and that which has been done has assessed the tissue under a limited number of cycles. 15 Understanding how the tissue responds to loading regimes more mimetic of daily life is necessary if we wish to succeed in ultimately replicating the meniscus with a functionally accurate synthetic surrogate or scaffold.…”

Section: Introductionmentioning

confidence: 99%

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Dynamic compression of human and ovine meniscal tissue compared with a potential thermoplastic elastomer hydrogel replacement

Fischenich

Boncella

Lewis

et al. 2017

J Biomedical Materials Res

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Understanding how human meniscal tissue responds to loading regimes mimetic of daily life as well as how it compares to larger animal models is critical in the development of a functionally accurate synthetic surrogate. Seven human and 8 ovine cadaveric meniscal specimens were regionally sectioned into cylinders 5mm in diameter and 3 mm thick along with 10 polystyrene-b-polyethylene oxide block copolymer-based thermoplastic elastomer (TPE) hydrogels. Samples were compressed to 12% strain at 1 Hz for 5000 cycles, unloaded for 24 hours, and then retested. No differences were found within each group between test one and test two. Human and ovine tissue exhibited no regional dependency (p<0.05). Human samples relaxed quicker than ovine tissue or the TPE hydrogel with modulus values at cycle 50 not significantly different from cycle 5000. Ovine menisci were found to be similar to human menisci in relaxation profile but had significantly higher modulus values (3.44MPa instantaneous and 0.61MPa after 5000 cycles compared to 1.97MPa and 0.11MPa found for human tissue) and significantly different power law fit coefficients. The TPE hydrogel had an initial modulus of 0.58MPa and experienced less than a 20% total relaxation over the 5000. Significant differences in the magnitude of compressive modulus between human and ovine menisci were observed, however the relaxation profiles were similar. Although statistically different than the native tissues, modulus values of the TPE hydrogel material were similar to those of the human and ovine menisci, making it a material worth further investigation for use as a synthetic replacement.

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Effects of degeneration on the compressive and tensile properties of human meniscus

Cited by 60 publications

References 26 publications

Meniscus mechanics and mechanobiology

Meniscus mechanics and mechanobiology

IL-10 ameliorates TNF-α induced meniscus degeneration in mature meniscal tissue in vitro

Dynamic compression of human and ovine meniscal tissue compared with a potential thermoplastic elastomer hydrogel replacement

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