Effects of Debaryomyces hansenii treatment on intestinal microorganisms in mice with antibiotics-induced diarrhea

He, Lu; Long, Chengxing; Liu, Youjia; Guo, Yan-Fang; Xiao, Nenqun; Tan, Zhoujin

doi:10.1007/s13205-017-0953-9

Cited by 22 publications

(25 citation statements)

References 18 publications

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“…Genomic DNAs from the intestinal contents were extracted by following the protocols (He et al 2017;Wu et al 2012). 2.0 g of intestinal feces was weighed in a sterile environment and was homogenized in 5 mL of 0.1 mol L −1 phosphate buffer solution (PBS), followed by centrifugation at 200 g for 2 min.…”

Section: Dna Extractionmentioning

confidence: 99%

Diversity of intestinal bacterial lactase gene in antibiotics-induced diarrhea mice treated with Chinese herbs compound Qi Wei Bai Zhu San

Liu

Guo

et al. 2017

3 Biotech

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The current investigation is trying to study the impact of the mixture of Chinese herbs Qi Wei Bai Zhu San (QWBZS) on bacterial lactase gene from antibiotics-induced diarrhea (AAD) mice, as the good curative effect of QWBZS on diarrhea. Mice (6 mice per group) were randomly selected as control, model and treatment groups. To induce diarrhea, mice in both model and treatment groups were intragastrically injected with mixture of gentamycin sulfate and cefradine (23.33 mL kg day) twice per day and continuously for totally 5 days. After the success of establishing diarrhea model, the mice in treatment group were gavaged with QWBZS for 3 days. Intestinal contents in all three groups were then collected and DNA was extracted in aseptic environment for the following sequencing. The results showed that mice from QWBZS treatment group had obviously detectable levels of intestinal bacteria, such as Actinobacteria, Firmicutes and Proteobacteria, which produce phyla lactase specifically. In comparison with other groups, the mice in treatment group had more abundant expression of lactase gene from sp. KKs102, sp. LMG11000, , and . Interestingly, the Shannon index decreased significantly after the treatment with QWBZS ( < 0.01 or < 0.05). 63.1% of lactase genes detected in the mice in treatment group were unclassified, and 32.8% of them were non-homologous to any fragments in the gene bank, which means that most of lactase-producing bacteria are novel. Our results indicate that treatment with QWBZS did not increase the diversity of bacterial lactase gene. Its curative effect on diarrhea may be relevant to its role in facilitating the growth of novel or some key lactase-producing strains.

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Section: Dna Extractionmentioning

confidence: 99%

Diversity of intestinal bacterial lactase gene in antibiotics-induced diarrhea mice treated with Chinese herbs compound Qi Wei Bai Zhu San

Liu

Guo

et al. 2017

3 Biotech

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“…Unfortunately, misuse of antibiotics frequently results in antibiotic-associated diarrhea in patients. The symptoms can be reduced or prevented using probiotics, such as D. hansenii [17].…”

Section: Discussionmentioning

confidence: 99%

“…The gut bacteria of the mice comprised Bacteroidetes, Firmicutes, Actinobacteria, Proteobacteria, and Verrucomicrobia at the phylum level [17]. Lactase-producing genes in the intestine are mainly derived from Actinobacteria, Firmicutes, Proteobacteria, and other, unclassified, species.…”

Section: Discussionmentioning

confidence: 99%

“…The total DNA of the intestinal content was extracted following previously reported protocols [17,23]. The intestinal contents were washed twice with phosphate buffer solution (PBS) (0.1 mol•L -1 ).…”

Section: Total Dna Extraction From Intestinal Contentsmentioning

confidence: 99%

“…Previous research on the impact of D. hansenii on AAD mainly focused on intestinal-cultured microbiota and various enzyme activities. A recent study reported that antibiotic-induced diarrhea was relieved by treatment with D. hansenii through adjustment of the richness and types of intestinal bacteria [17], although how D. hansenii affects bacterial functional genes is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Bacterial Lactase Gene Characteristics in Intestinal Contents of Antibiotic-Associated Diarrhea Mice Treated with Debaryomyces hansenii

Tang

Xiao

et al. 2020

Med Sci Monit

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Background: Debaryomyces hansenii exhibits a therapeutic effect on antibiotic-associated diarrhea (AAD) by promoting the growth of beneficial intestinal bacteria. Previous research has reported that AAD involves not only dysbacteriosis but also dysfunction of the activity of intestinal enzymes (such as lactase). Enzyme activities can be influenced by many other factors, such as gene expression. The present study showed that D. hansenii has a curative effect on AAD at the lactase gene level. Material/Methods: The effect of D. hansenii on the lactase gene from intestinal bacteria in AAD mice was investigated. The diarrhea model was established with a gentamycin sulfate and cefradine capsule mixture. The antibiotic mixture (23.33 mL•kg-1 •day-1) was intragastrically administered for 5 days. Subsequently, half of the diarrhea mice were treated with D. hansenii twice a day for 3 days while the other mice were intragastrically administered with the same volume of distilled water. Next, the intestinal contents were collected, and metagenomic DNA was extracted for high-throughput sequencing analysis. Results: The Chao1 and Shannon indices decreased significantly following treatment with D. hansenii (P<0.01 and P<0.05, respectively). Moreover, the clusters in the D. hansenii group mice were quite different from those in the normal group mice and model group mice. Following treatment with D. hansenii, the quantity of lactase genes in Enterobacter sp. 638 and Modestobacter increased markedly, and the richness of intestinal bacterial lactase genes in Fretibacterium recovered. Conclusions: D. hansenii altered the lactase-producing bacterial community structure and promoted the growth of several critical lactase-producing bacteria, such as Enterobacter sp. 638 and Modestobacter.

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Probiotic and nutritional effects of Debaryomyces hansenii on animals

Angulo

Reyes‐Becerril

Medina-Córdova

et al. 2020

Appl Microbiol Biotechnol

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Effects of Debaryomyces hansenii treatment on intestinal microorganisms in mice with antibiotics-induced diarrhea

Cited by 22 publications

References 18 publications

Diversity of intestinal bacterial lactase gene in antibiotics-induced diarrhea mice treated with Chinese herbs compound Qi Wei Bai Zhu San

Diversity of intestinal bacterial lactase gene in antibiotics-induced diarrhea mice treated with Chinese herbs compound Qi Wei Bai Zhu San

Bacterial Lactase Gene Characteristics in Intestinal Contents of Antibiotic-Associated Diarrhea Mice Treated with Debaryomyces hansenii

Probiotic and nutritional effects of Debaryomyces hansenii on animals

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