Effects of dahuangzhechong pills on cytokines and mitogen activated protein kinase activation in rats with hepatic fibrosis

Cai, Hong; Sun, Xiaoming; Liu, Zhi Feng; Liu, Ya Wei; Tang, Jing; Liu, Qiang; Bao, Junmin; Song, Yu; Zhou, Ying; Yang, Ming; Lv, Zhi Ping

doi:10.1016/j.jep.2010.08.019

Cited by 38 publications

(33 citation statements)

References 37 publications

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“…Western blot analysis was performed as previously described [24]. Briefly, the protein lysates were loaded onto 10% SDS-PAGE for separation, electrotransferred onto PVDF membranes, and blocked in 5% nonfat milk in Tris-buffered saline Tween.…”

Section: Methodsmentioning

confidence: 99%

Xiao Yao San Improves Depressive‐Like Behavior in Rats through Modulation of β‐Arrestin 2‐Mediated Pathways in Hippocampus

Zhu

Xia

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Xiao Yao San (XYS) is a classical Chinese medicine formula that has been widely used to treat mood disorders for hundreds of years. To confirm the effect of XYS and better understand its underlying mechanism, high-performance liquid chromatography-mass spectrometry analysis-based quality control of XYS extracts and proteomics-based identification of differential proteins in the hippocampus were adopted in social isolation and chronic unpredictable mild stress- (CUMS-) treated rats. The depressive-like behavior of rats induced by CUMS resembled the manifestation of human depression. The upregulated corticosterone (CORT) and urocortin 2 (UCN2) levels demonstrated the existence of hypothalamic-pituitary-adrenal (HPA) axis hyperactivity. XYS was effective in ameliorating the depressive-like behavior and downregulating UCN2 and CORT. XYS decreased the expression of serine/threonine-protein phosphatase 2A subunit B and increased the expression of β-arrestin 2. The expressions of brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB), and mammalian target of rapamycin (mTOR) were also elevated by XYS. In conclusion, XYS improves social isolation and CUMS-induced depressive-like behavior and ameliorates HPA hyperactivation through the downregulation of corticotrophin releasing hormone (CRH) receptor 2. The upregulation of BDNF/TrkB and the phosphorylation of mTOR require β-arrestin 2 as a scaffold to regulate stress signaling.

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Section: Methodsmentioning

confidence: 99%

Xiao Yao San Improves Depressive‐Like Behavior in Rats through Modulation of β‐Arrestin 2‐Mediated Pathways in Hippocampus

Zhu

Xia

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Therefore, Eupolyphaga sinensis Walker is also used to inhibit tumor growth and has potential in treating leukemia, gastric carcinoma and hepatocarcinoma. Some reports showed that Eupolyphaga sinensis Walker, one of the main components in Dahuang zhechong Pill, inhibited proliferation of vascular smooth muscle cells by depressing PDGF expression and alleviated CCl 4 -induced hepatic fibrosis by down-regulating p38 and ERK phosphorylation (Zhang et al, 2009;Cai et al, 2010 were tested for its antitumor effects in hepatocarcinoma H 22 bearing mice. Fatty acid compositions of the extract were also analyzed by GC-MS.…”

Section: Introductionmentioning

confidence: 99%

Antitumor effects and chemical compositions of Eupolyphaga sinensis Walker ethanol extract

et al. 2012

Journal of Ethnopharmacology

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“…(f) Nutrients BCAA-enriched diet in DEN treated rats (Cha et al 2013) Decreased mRNA levels for markers of fibrosis, angiogenesis, and apoptosis inhibition LA in TAA-treated rats (Foo et al 2011) Reduction in cirrhosis incidence, hepatic fibrosis, and AST/ALT activities Omega-3 in CCl4-treated rats (Shaaban et al 2014) Counteracting hepatic oxidative stress and augmenting hepatic antioxidants, reducing α-SMA expression, down-regulating PDGF-β expression, and inhibiting the fibrogenesis response (g) Traditional Chinese medicine In CCL4-induced fibrosis model, Panax notoginseng, Huisheng oral solution, saikosaponin-d, hydroxysafflor yellow A, Paeonia lactiflora and Astragalus, dahuangzhechong pills, and gomisin A were effective (Cai et al 2010;Dang et al 2007;Li et al 2014c;Peng et al 2009b;Sun et al 2007;Teraoka et al 2012;Zhang et al 2012) Reduced inflammatory cytokines, alleviated liver fibrosis…”

Section: Resultsmentioning

confidence: 99%

“…The anti-fibrotic effects of Paeonia lactiflora and Astragalus membranaceus (PAE) extract may be associated with its abilities to act as an antioxidant, to decrease TGF-β and collagen levels, and to inhibit HSC proliferation (Sun et al 2007). Da Huang Zhe Chong pill (DHZCP) can reverse and alleviate hepatic fibrosis by decreasing the secretion of TNF-α and IL-13 through the down-regulation of p38 and ERK phosphorylation (Cai et al 2010). Gomisin A is one of the major dibenzocyclooctadiene lignans isolated from Schisandra chinensis Baill; this treatment decreased liver oxidative stress by increasing superoxide dismutase activity and ameliorating inflammatory mediators (Teraoka et al 2012).…”

Section: Tgf-β Collagen and Attenuated Fibrosismentioning

confidence: 99%

Strategies to prevent and reverse liver fibrosis in humans and laboratory animals

Chen

Wang

2015

Arch Toxicol

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Liver fibrosis results from chronic damage to the liver in conjunction with various pathways and is mediated by a complex microenvironment. Based on clinical observations, it is now evident that fibrosis is a dynamic, bidirectional process with an inherent capacity for recovery and remodeling. The major mechanisms involved in liver fibrosis include the repetitive injury of hepatocytes, the activation of the inflammatory response after injury stimulation, and the activation and proliferation of hepatic stellate cells (HSCs), which represents the major extracellular matrix (ECM)-producing cells, stimulated by hepatocyte injury and inflammation. The microenvironment in the liver is synergistically regulated abnormal ECM deposition, scar formation, angiogenesis, and fibrogenesis. Moreover, recent studies have clarified novel mechanism in fibrosis such as epigenetic regulation of HSCs, the leptin and PPARγ pathways, the coagulation system, and even autophagy. Uncovering the mechanisms of liver fibrogenesis provides a basis to develop potential therapies to reverse and treat the fibrotic response, thereby improving the outcomes of patients with chronic liver disease. Although both scientific and clinical challenges remain, emerging studies attempt to reveal the ideal anti-fibrotic drug that could be easily delivered to the liver with high specificity and low toxicity. This review highlights the mechanisms, including novel pathways underlying fibrogenesis that may be translated into preventive and treatment strategies, reviews both current and novel agents that target specific pathways or multiple targets, and discusses novel drug delivery systems such as nanotechnology that can be applied in the treatment of liver fibrosis. In addition, we also discuss some current treatment strategies that are being applied in animal models and in clinical trials.

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Effects of dahuangzhechong pills on cytokines and mitogen activated protein kinase activation in rats with hepatic fibrosis

Cited by 38 publications

References 37 publications

Xiao Yao San Improves Depressive‐Like Behavior in Rats through Modulation of β‐Arrestin 2‐Mediated Pathways in Hippocampus

Xiao Yao San Improves Depressive‐Like Behavior in Rats through Modulation of β‐Arrestin 2‐Mediated Pathways in Hippocampus

Antitumor effects and chemical compositions of Eupolyphaga sinensis Walker ethanol extract

Strategies to prevent and reverse liver fibrosis in humans and laboratory animals

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