Effects of D-Limonene and α-D-Pinene on in Vitro Carbohydrate Dissimilation and Methane Formation by Rumen Bacteria

Crane, Alison R; Nelson, W.O.; Brown, Richard E.

doi:10.3168/jds.s0022-0302(57)94630-1

Cited by 24 publications

(13 citation statements)

References 7 publications

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“…Although bacteria which convert acetate to methane and carbon dioxide are readily established in enrichment cultures of bovine rumen contents, the data presented in this report demonstrate that only a small portion of rumen methane was derived from acetate and suggest that these organisms were present in small numbers, This observation is in accord with the report (Nelson, Brown & Kingwill, 1960) that the addition of d-limonene to the rumen in vivo in concentrations which were toxic to acetate-utilizing methane bacteria in vitro (Crane, Nelson & Brown, 1957) did not appreciably alter the ratio CO,:CH, in rumen gas. Beijer (1952) previously had observed that methane was not formed from acetate by rumen contents in vitro during short term incubation.…”

Section: Discussionsupporting

confidence: 80%

In vivo Studies of Methanogenesis in the Bovine Rumen: Dissimilation of Acetate

Oppermann

Nelson

Brown

1961

Journal of General Microbiology

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SUMMARYThe introduction of sodium acetate-2-14C into a bovine rumen resulted in the in vivo labelling of the rumen gases and volatile fatty acids. The relative isotope concentration in substrate and products indicated that a maximum of 5.6 % of the methane and 11% of the carbon dioxide in rumen gas might have been derived from the methyl carbon of acetate when the substrate was added to the rumen 18 hr. after the animal had been fed a normal ration, A maximum of 3-53 % of the methane and 4.2 % of the carbon dioxide might have been derived from the methyl carbon of sodium acetate-2-14C when this substrate was introduced into the rumen immediately after the animal had been fed. The addition of sodium acetate-l-14C to the rumen 18 hr. after feeding indicated that 2 yo of the methane and 10 yo of the carbon dioxide was derived from the carboxyl carbon of the substrate. Most of the derived radioactivity of the volatile acids of the rumen was found in the butyric acid fraction, although smaller amounts appeared in propionic acid and the volatile fatty acids with a chain length of greater than 4 carbons.

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Section: Discussionsupporting

confidence: 80%

In vivo Studies of Methanogenesis in the Bovine Rumen: Dissimilation of Acetate

Oppermann

Nelson

Brown

1961

Journal of General Microbiology

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“…However, according to some authors, it is mainly the used amount and the source of essential oils that are responsible for the result of reduction or lack of reduction of methane production (Evans and Martin, 2000;Chaves et al, 2008). Crane et al (1957) demonstrated that the addition of a secondary plant metabolite, limonene, can limit the growth of the microorganisms participating in methane formation in the rumen, thus resulting in the reduction of its production. The research by McIntosh et al (2003) showed that the limitation on the number of Methanobrevibacter smithii (measured indirectly through the amount of produced methane) occurred only after using the highest addition (1000 ppm) of the commercial mixture of essential oils, while the lower doses (5, 10, 20, 40, 80, 160 ppm) did not adversely affect the population of Methanobrevibacter smithii.…”

Section: The Effect Of Essential Oils On the Process Of Methanogenesimentioning

confidence: 99%

Potential of phytofactors to mitigate rumen ammonia and methane production

Szumacher-Strabel¹,

Cieślak²

2010

J. Anim. Feed Sci.

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The increase in the intensity of ruminant production determines changes in the rumen feed conversion and thus, among other, may increase pool of greenhouse gases emission. The ineffective digestion process and changes in the legislation processes implicate the action tends towards the limitation of rumen ammonia and methane production. In the last few years there is an increasing interest of nutritionists in bioactive plant factors -phytofactors as natural feed additives that can modify the rumen fermentation processes, improve the protein metabolism and, at the same time, reduce ammonia production and emission, and curb methane production and emission to the atmosphere. High diversity of bioactive phytofactors contained in many plant species has been identified as a potential factor affecting the above-mentioned processes.

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“…According to the biochemical reaction series of anaerobic digestion, methane is formed from acetate (by acetogenic methanogens) and H2/CO2 (by hydrogenotrophic methanogens) [6]. Limonene was found to be inhibitory more for acetogenic methanogens rather than hydrogenotrophic methanogens [9]. Acetic acid consumption was inhibited in the presence of a limonene concentration of 9.10-4 M. Limonene being hydrophobic was prevented diffusing into the hydrophilic membrane.…”

Section: Production Of Methane In the 2 Nd Stage Of Anaerobic Digestimentioning

confidence: 90%

Anaerobic digestion of citrus waste using two-stage membrane bioreactor

Millati

Lukitawesa

Permanasari

et al. 2018

IOP Conf. Ser.: Mater. Sci. Eng.

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Abstract. Anaerobic digestion is a promising method to treat citrus waste. However, the presence of limonene in citrus waste inhibits anaerobic digestion process. Limonene is an antimicrobial compound and could inhibit methane forming bacteria that takes a longer time to recover than the injured acid forming bacteria. Hence, volatile fatty acids will be accumulated and methane production will be decreased. One way to solve this problem is by conducting anaerobic digestion process into two stages. The first step is aimed for hydrolysis, acidogenesis, and acetogenesis reactions and the second stage is aimed for methanogenesis reaction. The separation of the system would further allow each stage in their optimum conditions making the process more stable. In this research, anaerobic digestion was carried out in batch operations using 120 ml-glass bottle bioreactors in 2 stages. The first stage was performed in free-cells bioreactor, whereas the second stage was performed in both bioreactor of free cells and membrane bioreactor. In the first stage, the reactor was set into 'anaerobic' and 'semi-aerobic' conditions to examine the effect of oxygen on facultative anaerobic bacteria in acid production. In the second stage, the protection of membrane towards the cells against limonene was tested. For the first stage, the basal medium was prepared with 1.5 g VS of inoculum and 4.5 g VS of citrus waste. The digestion process was carried out at 55°C for four days. For the second stage, the membrane bioreactor was prepared with 3 g of cells that were encased and sealed in a 3×6 cm 2 polyvinylidene fluoride membrane. The medium contained 40 ml basal medium and 10 ml liquid from the first stage. The bioreactors were incubated at 55°C for 2 days under anaerobic condition. The results from the first stage showed that the maximum total sugar under 'anaerobic' and 'semi-aerobic' conditions was 294.3 g/l and 244.7 g/l, respectively. The corresponding values for total volatile fatty acids were 3.8 g/l and 2.9 g/l, respectively. Methane production of citrus waste taken from the first stage under 'anaerobic' condition in membrane and free-cells bioreactors was 11.2 Nml and 7.2 Nml, respectively. Whereas, methane production of citrus waste taken from the first stage under 'semi-aerobic' condition in membrane and free-cells bioreactors was 8.8 Nml and 5.7 Nml, respectively. It can be seen from the results of the first stage that volatile fatty acids from 'anaerobic' condition was higher than that of 'semi-aerobic' condition. The absence of oxygen provides the optimal condition for growth and metabolism of facultative and obligatorily anaerobic bacteria in the first stage. Furthermore, polyvinylidene fluoride membrane was able to protect the cells from antimicrobial compounds.

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Effects of D-Limonene and α-D-Pinene on in Vitro Carbohydrate Dissimilation and Methane Formation by Rumen Bacteria

Cited by 24 publications

References 7 publications

In vivo Studies of Methanogenesis in the Bovine Rumen: Dissimilation of Acetate

In vivo Studies of Methanogenesis in the Bovine Rumen: Dissimilation of Acetate

Potential of phytofactors to mitigate rumen ammonia and methane production

Anaerobic digestion of citrus waste using two-stage membrane bioreactor

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