Effects of cytochalasin H on the embryonic endodermal cells of Microhyla ornata

Joshi, Manmohan V.; Mulherkar, Leela

doi:10.1007/bf01951302

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…It was sug gested that Con A capping involves the active movement of unbound membrane incident to locomotion or pseudopodium formation [7], The occurence of capping in CH-treated cells and our observations using light micros copy on pseudopodium formation even after prolonged CH treatment clearly indicate that the primary effect of CH is not on cell move ment. The occurrence of capping in CHtreated sponge cells, however, appears to be at variance with the reported inhibition of capping of Con A by CB in lymphocytes [4], This may be due to the difference in the con tractile apparatus in different cell types as suggested by Godman and Miranda [7], Disaggregating effects of CH on the em bryonic cells of the frog, Microhyla ornata, have been previously discussed at length [21,40,41], The results of these and our present study with exogenous administration of glu cosamine provide convincing evidence that CH, like CB [35], could be interfering with the production, release or binding of carbo hydrate-containing macromolecules which are important in cell aggregation.…”

Section: Resultscontrasting

confidence: 44%

“…31. 40], to bring about enucleation [31] and to cause disaggregation of em bryonic cells [21,40], Disaggregated cells of sponges are able to reaggregate due to the aggregating factor present on sponge cells [ 16,18,28,29], However, cytochalasins A. B and E have been shown to inhibit this reaggrega tion [12,13] although the exact mechanism is not fully understood.…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory Effect of Cytochalasin H on Cell Reaggregation of the Fresh Water Sponge, <i>Ephydatia meyeni </i>Carter

Ghaskadbi¹,

Mulherkar²

1982

Pathobiology

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Cytochalasin H (CH) has been shown to inhibit the reaggregation of dissociated cells of a fresh water sponge. The effect is dose-dependent and reversible. Even after 24 h of CH treatment, the cells show the formation of pseudopodia suggesting that the effect of CH may not be due to arrested cell motility. Exogenously added α-D-glucosamine, a precursor of complex carbohydrates, brings about reaggregation of CH-treated cells. Fluorescent Con A binding, both patching and capping, is shown by untreated as well as CH-treated cells. It is likely that CH, like cytochalasin B, may interfere with the production, release or binding of carbohydrate-containing macromolecules important in cell aggregation.

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Section: Resultscontrasting

confidence: 44%

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Cytochalasin H on Cell Reaggregation of the Fresh Water Sponge, <i>Ephydatia meyeni </i>Carter

Ghaskadbi¹,

Mulherkar²

1982

Pathobiology

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The effect of cytochalasin B on chick mesoderm cells as studied by scanning electron microscopy

1979

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The effects of cytochalasin B (CCB) on chick mesoderm cells in vivo was examined by scanning electron microscopy (SEM). The embryos were mounted for New Culture and the mesoderm exposed by dissecting off the endoderm. Cytochalasin B was suspended in saline and the embryos flooded with the suspension. Control embryos were treated with saline alone. The embryos were reincubated for varying times at 37 degrees C. In the treated embryos the mesoderm cells were rounded and separated from each other. Many had long branched processes and rough surfaces. These changes became more pronounced as treatment time was increased. They were also reversible on reincubating treated embryos in the absence of cytochalasin B. The morphological changes produced by CCB are thought to be due to an effect on the cytoskeleton, either a direct disruptive effect or detachment of skeletal microfilaments from the cell membrane. There may also be a direct removal of cell surface materials leading to the observed surface roughening of treated cells.

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Effects of cytochalasin H on developing frog embryos: reversibility of action

Wadekar

Ghaskadbi²,

Mulherkar

1987

Roux's Arch Dev Biol

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Cytochalasin H (CH) brought about cellular disaggregation resulting into arrest of development in the embryos of the frog, Microhyla ornata. The effects were dependent on the dose of CH, duration of the treatment as well as on developmental stage of the embryo at the beginning of the treatment. The embryos were protected to some extent if the vitelline membranes were left intact. The reversibility of CH-action, after transfer of treated embryos to CH-free medium, was dependent on the extent of cellular disaggregation brought about by CH and on the presence or absence of an intact vitelline membrane.

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Effects of cytochalasin H on the embryonic endodermal cells of Microhyla ornata

Cited by 4 publications

References 14 publications

Inhibitory Effect of Cytochalasin H on Cell Reaggregation of the Fresh Water Sponge, <i>Ephydatia meyeni </i>Carter

Inhibitory Effect of Cytochalasin H on Cell Reaggregation of the Fresh Water Sponge, <i>Ephydatia meyeni </i>Carter

The effect of cytochalasin B on chick mesoderm cells as studied by scanning electron microscopy

Effects of cytochalasin H on developing frog embryos: reversibility of action

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