Effects of Cyclodextrins on Chemically and Thermally Induced Unfolding and Aggregation of Lysozyme and Basic Fibroblast Growth Factor

Tavornvipas, Sumitra; Hirayama, Fumitoshi; Takeda, Shigeaki; Arima, Hisatomi; Uekama, Kaneto

doi:10.1002/jps.20715

Cited by 49 publications

(34 citation statements)

References 40 publications

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“…As a result, research on this subject has attracted the attention of biologists, chemists, pharmacists, and therapists. [6][7][8][9][10][11][12] Protein stability and folding pathways are closely dependent on the various solvent ionic compositions, temperature, and adsorption on a hydrophobic surface. Excipients/additives, including cyclodextrins (CDs), can be added to the protein preparation commonly in order to inhibit protein denaturation.…”

Section: Introductionmentioning

confidence: 99%

“…Protein stability is a particularly relevant issue in the pharmaceutical field and will continue to gain more importance as the number of therapeutic protein products increases. [5,6] Interactions between serum albumin and ligands can provide important information about ligand storage, transportation, evacuation, etc. As a result, research on this subject has attracted the attention of biologists, chemists, pharmacists, and therapists.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the Effects of Various Cyclodextrins on the Stabilisation of Human Serum Albumin by a Spectroscopic Method

et al. 2015

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The binding parameters between cyclodextrins (CDs) and human serum albumin (HSA) were investigated by isothermal titration calorimetry (ITC), fluorescence quenching, and UV-vis absorption spectroscopy at 300 K in 50 mM phosphate buffer solution. Among the various CDs investigated, b-CD has the greater ability to decrease the aggregation of HSA and the results indicated that the inhibition order is g-CD , a-CD , b-CD. The obtained heats for HSAþCDs interactions were reported and analysed in terms of the extended solvation model, which was used to reproduce the enthalpies of HSA interactions with CDs over a broad range of complex concentrations. The binding constant and thermodynamic parameters were obtained. These suggested that the binding reaction was driven by both enthalpy and entropy, and electrostatic interactions played a major role in the stabilising of HSA. The parameters d y A and d y B reflected the net effect of b-CD on the HSA stability at low and high cyclodextrin concentrations, respectively. The positive values for d y A indicated that b-CD stabilises the HSA structure at low concentrations. The UV absorption intensity of theses complexes increased and a slight red shift was observed in the absorbance wavelength with increasing the CD concentration. The fluorescence intensity of HSA decreased regularly and a slight blue shift was observed for the emission wavelength with increasing CD concentration. The results indicate that the CD complex could quench the fluorescence of HSA and changes the microenvironment of the tryptophan residue.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigation of the Effects of Various Cyclodextrins on the Stabilisation of Human Serum Albumin by a Spectroscopic Method

et al. 2015

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“…11). 147 The authors rationalized the result in terms of the better fitting of aromatic amino acid residues inside the bCD cavity, favoring the formation of poly(Mal-bCD):protein inclusion complexes. Complexation was actually found to destabilize the native conformation of the protein, favouring a partially unfolded state.…”

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confidence: 96%

Cyclodextrin-based multivalent glycodisplays: covalent and supramolecular conjugates to assess carbohydrate–protein interactions

2013

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“…CyDs have been claimed to interact with hydrophobic residues exposed on protein surfaces and thereby to decrease the aggregation of proteins [22, 23]. We previously reported that SBE4- β -CyD inhibited the aggregation of bovine insulin in neutral solution, possibly due to the interaction of SBE4- β -CyD with aromatic side chain of insulin such as B26-tyrosine, A19-tyrosine, B1-phenylalanine, and B25-phenylalanine [17].…”

Section: Resultsmentioning

confidence: 99%

Effects of Selected Anionic β-Cyclodextrins on Persistence of Blood Glucose Lowering by Insulin Glargine after Subcutaneous Injection to Rats

Uehata

Anno

Hayashida

et al. 2011

Journal of Drug Delivery

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Insulin glargine is a synthetic long-acting insulin product used for patients with diabetes mellitus. In this study, to obtain the further desirable blood-glucose lowering profile of insulin glargine, we investigated the effects of β-cyclodextrin sulfate (Sul-β-CyD) and sulfobutylether β-cyclodextrin (SBE7-β-CyD) on physicochemical properties of insulin glargine and pharmacokinetics/pharmacodynamics of insulin glargine after subcutaneous injection to rats. Sul-β-CyD and SBE7-β-CyD increased solubility of insulin glargine. SBE7-β-CyD suppressed the formation of oligomer and enhanced the dissolution rate of insulin glargine from its precipitate, compared to that of Sul-β-CyD. Additionally, we revealed that after subcutaneous administration of an insulin glargine solution, SBE7-β-CyD, but not Sul-β-CyD, increased bioavailability and sustained the blood-glucose lowering effect, possibly due to the inhibitory effects of SBE7-β-CyD on the enzymatic degradation at the injection site. These results suggest that SBE7-β-CyD could be a useful excipient for sustained release of insulin glargine.

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Effects of Cyclodextrins on Chemically and Thermally Induced Unfolding and Aggregation of Lysozyme and Basic Fibroblast Growth Factor

Cited by 49 publications

References 40 publications

Investigation of the Effects of Various Cyclodextrins on the Stabilisation of Human Serum Albumin by a Spectroscopic Method

Investigation of the Effects of Various Cyclodextrins on the Stabilisation of Human Serum Albumin by a Spectroscopic Method

Cyclodextrin-based multivalent glycodisplays: covalent and supramolecular conjugates to assess carbohydrate–protein interactions

Effects of Selected Anionic β-Cyclodextrins on Persistence of Blood Glucose Lowering by Insulin Glargine after Subcutaneous Injection to Rats

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