eCM 2004
DOI: 10.22203/ecm.v007a04
|View full text |Cite
|
Sign up to set email alerts
|

Effects of cyclic longitudinal mechanical strain and dexamethasone on osteogenic differentiation of human bone marrow stromal cells

Abstract: The aim of the study was to investigate the effect of cyclic mechanical strain on differentiation markers in the presence or absence of dexamethasone. Human bone marrow stromal cells (BMSC) from seven donors (32.5±6.2 years) were cultivated with (D+) or without (D-) dexamethasone. A cyclic mechanical strain with an elongation of 2% (D+2; D-2) or 8% (D+8; D-8) was applied for three days with a stimulation time of three times two hours each day. Levels of alkaline phosphatase (ALP) and osteocalcin (OC) were comp… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

2
100
0
1

Year Published

2008
2008
2018
2018

Publication Types

Select...
9

Relationship

0
9

Authors

Journals

citations
Cited by 133 publications
(103 citation statements)
references
References 17 publications
2
100
0
1
Order By: Relevance
“…Hence, the response of MSCs to mechanical loading followed current models that suggest that compressive load supports chondrogenic MSC differentiation ( Table 2) (Campbell et al, 2006;Mauck et al, 2007;Pelaez et al, 2009). In contrast, osteogenic differentiation of MSCs was unaffected by cyclic compression (3D) adding weight to the notion that osteogenic differentiation requires a different mechanical stimulus, such as cyclic equibiaxial/uniaxial strain (2D) or four-point bending (Table 2) (David et al, 2007;Jagodzinski et al, 2004;Koike et al, 2005;Mauney et al, 2004). Little is known about the function of CD73 in MSCs thus far.…”
Section: Discussionmentioning
confidence: 84%
“…Hence, the response of MSCs to mechanical loading followed current models that suggest that compressive load supports chondrogenic MSC differentiation ( Table 2) (Campbell et al, 2006;Mauck et al, 2007;Pelaez et al, 2009). In contrast, osteogenic differentiation of MSCs was unaffected by cyclic compression (3D) adding weight to the notion that osteogenic differentiation requires a different mechanical stimulus, such as cyclic equibiaxial/uniaxial strain (2D) or four-point bending (Table 2) (David et al, 2007;Jagodzinski et al, 2004;Koike et al, 2005;Mauney et al, 2004). Little is known about the function of CD73 in MSCs thus far.…”
Section: Discussionmentioning
confidence: 84%
“…ON is synthesized by cells of the osteoblastic lineage, and it also is a differentiation marker of bone cells [46]. Col I, an important component of the bone extracellular matrix, has been shown to be involved in the differentiation of the osteoblast phenotype [47]. LiCl is able to increase osteoblast differentiation [48]; thus it was used as a positive control.…”
Section: Effect Of -Tocotrienol On the Differentiation Of Mc3t3-e1mentioning
confidence: 99%
“…Stimulation of human mesenchymal stem cells (hMSCs) by tensile strain and osteogenic media conditions to induce osteogenic differentiation has been widely investigated (Simmons et al, 2003;Jagodzinski et al, 2004;Koike et al, 2005;Sumanasinghe et al, 2006). Many investigations have focused on determining appropriate strain levels (Simmons et al, 2003;Jagodzinski et al, 2004;Loboa et al, 2005;Koike et al, 2005;Sumanasinghe et al, 2006) and media supplements Jaiswal et al, 1997;Halvorsen et al, 2001) required to achieve osteogenesis of hMSCs.…”
mentioning
confidence: 99%
“…Many investigations have focused on determining appropriate strain levels (Simmons et al, 2003;Jagodzinski et al, 2004;Loboa et al, 2005;Koike et al, 2005;Sumanasinghe et al, 2006) and media supplements Jaiswal et al, 1997;Halvorsen et al, 2001) required to achieve osteogenesis of hMSCs. Tensile strains of 10-12% have been shown to initiate intramembranous bone formation in rats (Loboa et al, 2004(Loboa et al, , 2005.…”
mentioning
confidence: 99%