Effects of cyclic AMP, ouabain and furosemide on ion transport in isolated canine gastric mucosa.

Abstract

trations inhibited the net flux of Cl-from the serosal to mucosal side. 5. These results suggest that in isolated dog gastric mucosa, (1) both DBcAMP and theophylline may increase intracellular cyclic AMP to stimulate H+ and C1-secretion, (2) cyclic AMP, outside the serosal membrane, stimulates active transport of Na+; in contrast, ouabain inhibits this active process predominantly, (3) the selective action of furosemide on Na+ transport indicates that Na+ and Cl-move via separate transport pathways across the serosal border.

Usefulness of a novel Caco-2 cell perfusion system. I. in vitro prediction of the absorption potential of passively diffused compounds

“…Theophylline may also increase intracellular cyclic AMP to stimulate H + and Cl ‐ secretion. Cyclic AMP, outside the serosal membrane, stimulates active transport of Na + 34…”

Distribution of carbonic anhydrase in cells and membranes isolated from pig gastric mucosa

“…T h e inhibition of gastric acid secretion by furosemide in isolated gastric mucosa of the guinea pig is reported to be 38% but is attributed to the inhibition of C1transport through the basolateral membrane of the parietal cell (Ayalon et al 1980). K u o andShanbour (1980) reported inhibitory effects of furosemide on Na+ and C1-fluxes in isolated canine gastric mucosa. In the present study, the K, for furosemide inhibition of carbonic anhydrase in the gastric vesicles was similar to the valued reported in the literature for other carbonic anhydrases (Cousin & Motais 1976).…”