Effects of cyanobacteria Oscillatoria sp. lipopolysaccharide on B cell activation and Toll-like receptor 4 signaling

Swanson‐Mungerson, Michelle; Incrocci, Ryan; Subramaniam, Vijay; Williams, Philip G.; Hall, Melanie; Mayer, Alejandro M. S.

doi:10.1016/j.toxlet.2017.05.013

Cited by 31 publications

(27 citation statements)

References 34 publications

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“…We observed that ethanol and acetone extracts had an opposite behavior to one another-all ethanol extracts increased the levels of NO produced by RAW 264.7 on their own, suggesting a pro-inflammatory potential ( Figure S1) and thus not meeting the aim of our study. This phenomena could have occurred, at least in part, as a result of the presence of cyanobacteria endotoxins (LPS), as these molecules are more prone to being extracted from more polar solvent mixtures (e.g., ethanol 70% v/v), causing macrophage activation through interactions with Toll-like receptors (TLR4) present in cell membranes [45]. Of the acetone extracts, those of C. issatschenkoi LEGE03282 and C. gracile LEGE12431 also presented pro-inflammatory behavior ( Figure S2), and only three were able to reduce the levels of NO after LPS stimulation ( Figure 2).…”

Section: Anti-inflammatory Potentialmentioning

confidence: 99%

“…causing macrophage activation through interactions with Toll-like receptors (TLR4) present in cell membranes [45]. Of the acetone extracts, those of C. issatschenkoi LEGE03282 and C. gracile LEGE12431 also presented pro-inflammatory behavior ( Figure S2), and only three were able to reduce the levels of NO after LPS stimulation (Figure 2).…”

Section: Anti-inflammatory Potentialmentioning

confidence: 99%

“…HPLC-PDA recorded at 450 nm. Unidentified carotenoids (3, 5-8, 10-13, 15, 23, 25, 28, 31, 40-43), β-carotene oxygenated derivatives (9, 17, 34, 35), Lutein derivatives(14,16,19,22,24), Echinenone derivatives(30), Chlorophyll a derivatives(32,33,38), Unidentified Chlorophylls(44)(45)(46)(47), Zeaxanthin(21), Canthaxanthin(26), Chlorophyll a(36), Echinenone(37), all-trans β-Carotene (48), α-Carotene derivative (49), 13-cis-β-Carotene(50).…”

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confidence: 99%

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Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis

2020

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In this study, five cyanobacteria strains (Alkalinema aff. pantanalense LEGE15481, Cyanobium gracile LEGE12431, Nodosilinea (Leptolyngbya) antarctica LEGE13457, Cuspidothrix issatschenkoi LEGE03282 and Leptolyngbya-like sp. LEGE13412) from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE CC) of CIIMAR were explored for their biotechnological potential in the treatment of psoriasis. Different extracts were characterized for their pigment profile by HPLC-PDA. The antioxidant potential of the extracts was assessed against the superoxide anion radical (O2•-). Their anti-inflammatory and antiproliferative potential was assessed in vitro using the macrophages RAW 264.7 and the human keratinocytes HaCaT as cell-line models, respectively. Terrestrial and freshwater strains presented the highest carotenoid content (33193−63926 μg/g dry extract), with all-trans-β-carotene, zeaxanthin, echinenone and lutein derivatives being the most abundant carotenoids. Acetone was the most effective solvent for pigment extraction. The acetone extracts presented the lowest IC50 values (0.29−0.38 mg dry extract/mL) regarding O2•- scavenging, and revealed anti-inflammatory potential, with N. antarctica LEGE13457, A. pantanalense LEGE15481 and Leptolyngbya-like sp. LEGE13412 reducing the nitric oxide (NO) in RAW 264.7 cell culture medium in about 25% (p < 0.05). With the exception of A. pantanalense LEGE15481, all the extracts significantly reduced keratinocyte proliferation (p < 0.05), demonstrating a selective toxicity among the different cell lines. Overall, Leptolyngbya-like sp. LEGE13412 and N. antarctica LEGE13457 seem promising for further exploitation in the framework of psoriasis, due to their antioxidant, anti-inflammatory and antiproliferative potential.

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Section: Anti-inflammatory Potentialmentioning

confidence: 99%

Section: Anti-inflammatory Potentialmentioning

confidence: 99%

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confidence: 99%

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Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis

2020

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“…In another cadmium project, the role of cadmium in the development of diabetes and diabetic nephropathy was examined. It was shown that cadmium accumulates in the pancreas, increases blood glucose levels, and [26][27][28] From the One Health project focused on cyanobacteria in fresh water, several articles [29][30][31] have documented the effect of cyanobacterium The One Health Initiative has also provided ample opportunities for students to engage in research.…”

Section: History Of Mwu/ccom and Its Tradition Of Researchmentioning

confidence: 99%

The One Health Initiative as a Basis for Research Development in the Department of Pharmacology at Midwestern University

Prozialeck¹,

Edwards²

2018

Journal of Osteopathic Medicine

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The One Health Initiative focuses on the complex relationships among the health of humans, animals, plants, microbes, and the environment. There are dynamic and delicate balances among these various elements, and disruption of these elements can have adverse effects on human health. Over the past 5 years, the Department of Pharmacology at the Midwestern University/Chicago College of Osteopathic Medicine has used the One Health Initiative as a framework for the growth and development of ongoing research programs in the area of environmental toxicology. As described in this article, this One Health approach has been successful, as evidenced by increases in the number of publications and level of grant-seeking activity by department faculty. With its emphasis on holistic patient care, the osteopathic medical profession is well positioned to be a leading advocate for the One Health Initiative.

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“…Toll like receptor (TLR) is a type of inherent immune receptor and may be activated by its ligands, such as lipopolysaccharide (LPS) (8). The downstream signaling pathway consists of recruiting bone marrow myeloid differentiation protein gene 88 (MyD88) and activating the tumor necrosis factor-κB (NF-κB) p65 signaling pathway, which regulates the expression of inflammatory cytokines and promotes the immune response (9,10).…”

Section: Introductionmentioning

confidence: 99%

Effects of Tim-3 silencing on the viability of fibroblast-like synoviocytes and lipopolysaccharide-induced inflammatory reactions

Long

Chen

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. The objective of the present study was to investigate the effects of Tim-3 silencing on cell viability and lipopolysaccharide (LPS)-induced inflammatory reactions in fibroblast-like synoviocytes (FLS). T-cell immunoglobulin mucin domain molecule (Tim)-3 expression in FLS obtained from patients with rheumatoid arthritis (RA) and normal controls were detected by western blot analysis and reverse transcription-polymerase chain reaction (RT-PCR). Small interfering (si)RNA was transfected using Lipofectamine ® 2000 to decrease Tim-3 expression. Following transfection, FLS were stimulated by LPS. An MTT assay, RT-PCR and western blot analysis were performed to measure cell viability, Toll-like receptor 4 (TLR4) signaling pathway-related protein expression and inflammatory cytokine release, respectively. The results of the present study indicated that Tim-3 expression was increased in FLS from patients with RA compared with FLS from healthy controls. Transfection of Tim-3 siRNA significantly decreased Tim-3 expression in FLS from patients with RA. Notably, Tim-3 silencing decreased FLS cell viability. Following stimulation with LPS, cell viability and the expression of TLR4, myeloid differentiation protein gene 88 (MyD88) and nuclear factor-κB (NF-κB) p65 were enhanced in FLS. By contrast, Tim-3 silencing attenuated LPS-induced cell proliferation and the expression of TLR4, MyD88 and NF-κB p65. In addition, LPS significantly increased levels of cytokines in the supernatant, including tumor necrosis factor-α, interferon-γ and interleukin-6 (P<0.01). By contrast, Tim-3 silencing significantly decreased LPS-induced cytokine release (P<0.01). However, Tim-3 silencing did not affect TLR4, MyD88 and NF-κB p65 expression and the release of cytokines in cells that did not undergo treatment with LPS. Therefore, the results of the present study indicate that Tim-3 silencing decreases the viability of FLS in RA and attenuates the LPS-induced inflammatory reaction.

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Effects of cyanobacteria Oscillatoria sp. lipopolysaccharide on B cell activation and Toll-like receptor 4 signaling

Cited by 31 publications

References 34 publications

Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis

Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis

The One Health Initiative as a Basis for Research Development in the Department of Pharmacology at Midwestern University

Effects of Tim-3 silencing on the viability of fibroblast-like synoviocytes and lipopolysaccharide-induced inflammatory reactions

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