Effects of cyanidin 3-<i>O</i>-glucoside and hydrochlorothiazide on T-cell phenotypes and function in spontaneously hypertensive rats

Aloud, Basma Milad; Petkau, Jay C.; Yu, Liping; McCallum, Jason; Kirby, Christopher W.; Netticadan, Thomas; Blewett, Heather

doi:10.1039/d0fo01778g

Cited by 8 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spontaneously hypertensive rats (SHR) when treated with hydrochlorothiazide increases the splenic expression of CD62L hi (naive) and CD62L lo (memory) T cells, but reduces expression of CD62L(-) effector T cells in comparison to the control group of SHR. The percentage of regulatory T and Th CD25+ splenocytes were not affected with hydrochlorothiazide treatment of SHR [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

“…Hydrochlorothiazide reduces the concentrations of TNFα and IFNγ in supernatants from cell culture of hydrochlorothiazide treated splenocytes obtained from SHR [ 62 ]. Our studies have shown that hydrochlorothiazide slightly increases the concentration of the pro-inflammatory cytokine TNFα, however, adding captopril causes a significant reduction in the production of TNFα.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response

Bryniarski

Nazimek

Marcinkiewicz

2021

IJMS

View full text Add to dashboard Cite

Hypertension is accompanied by the over-activation of macrophages. Diuretics administered alone or in combination with hypotensive drugs may have immunomodulatory effects. Thus, the influence of tested drugs on mouse macrophage-mediated humoral immunity was investigated. Mice were treated intraperitoneally with captopril (5 mg/kg) with or without hydrochlorothiazide (10 mg/kg) or furosemide (5 mg/kg) by 8 days. Mineral oil-induced peritoneal macrophages were harvested to assess the generation of cytokines in ELISA, and the expression of surface markers was analyzed cytometrically. Macrophages were also pulsed with sheep red blood cells (SRBC) and transferred to naive mice for evaluation of their ability to induce a humoral immune response. Tested drugs increase the expression of surface markers important for the antigen phagocytosis and presentation. SRBC-pulsed macrophages from mice treated with captopril combined with diuretics increased the secretion of antigen-specific antibodies by recipient B cells, while macrophages of mice treated with hydrochlorothiazide or furosemide with captopril increased the number of antigen-specific B cells. Tested drugs alter the macrophage secretory profile in favor of anti-inflammatory cytokines. Our results showed that diuretics with or without captopril modulate the humoral response by affecting the function of macrophages, which has significant translational potential in assessing the safety of antihypertensive therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response

Bryniarski

Nazimek

Marcinkiewicz

2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Hydrochlorothiazide inhibits T cell function in spontaneous hypertension [ 72 ]. This diuretic was found to inhibit the production of nitric oxide [ 17 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

Captopril Combined with Furosemide or Hydrochlorothiazide Affects Macrophage Functions in Mouse Contact Hypersensitivity Response

Bryniarski

Nazimek

Marcinkiewicz

2021

IJMS

View full text Add to dashboard Cite

Hypertension is a chronic disease associated with chronic inflammation involving activated macrophages. Antihypertensive drugs (for example, angiotensin-converting enzyme inhibitors—ACEIs) used in the treatment of hypertension have immunomodulatory properties. On the other hand, the immunological effect of diuretics and combined drugs (diuretics + ACEI) is unclear. Therefore, we examined the influence of diuretics and combination drugs (ACEI + diuretic) on cellular response (contact hypersensitivity), production of reactive oxygen intermediates (ROIs), and nitric oxide (NO), and the secretion of interleukin-12 (IL-12). CBA mice were administered i.p. captopril (5 mg/kg) with or without hydrochlorothiazide (10 mg/kg) or furosemide (5 mg/kg) for 8 days. On the third day, the mice were administered i.p. mineral oil, and macrophages were collected 5 days later. In the presented results, we show that diuretics administered alone or with captopril increase the generation of ROIs and reduce the formation of NO by macrophages. Moreover, tested drugs inhibit the secretion of IL-12. Diuretics and combined drugs reduce the activity of contact hypersensitivity (both activation and induction phases). Our research shows that the tested drugs modulate the cellular response by influencing the function of macrophages, which is important in assessing the safety of antihypertensive therapy.

show abstract

“…The reduced satellite cell pool of thymectomized mice prompted us to speculate that active factors are released from the thymus. As previous reports have shown that concanavalin (ConA)-stimulated T cells are capable of promoting satellite cell proliferation by releasing cytokines (IL-2, IL-10, TNFα, and IFNγ) (42,43), we cultured rat thymus cells in vitro for 48 h with or without activation by ConA and collected unactivated thymocyte-conditioned medium (TCM) and activated TCM (TCM-ConA). When cultured with TCM for 5 days, the satellite cells started to grow rapidly in small spheres (cell aggregates).…”

Section: Thymus Cell Culture Directly Promotes Satellite Cell Amplifi...mentioning

confidence: 99%

The thymus regulates skeletal muscle regeneration by directly promoting satellite cell expansion

Zheng

Wang

Chen

et al. 2022

Journal of Biological Chemistry

View full text Add to dashboard Cite

Effects of cyanidin 3-O-glucoside and hydrochlorothiazide on T-cell phenotypes and function in spontaneously hypertensive rats

Abstract: Immune system dysfunction may contribute to the pathogenesis of hypertension in spontaneously hypertensive rats (SHR). We examined the effects of the anthocyanin, cyanidin 3-O-glucoside (C3G), and the diuretic, hydrochlorothiazide (HCT),...

Cited by 8 publications

References 24 publications

Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response

Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response

Captopril Combined with Furosemide or Hydrochlorothiazide Affects Macrophage Functions in Mouse Contact Hypersensitivity Response

The thymus regulates skeletal muscle regeneration by directly promoting satellite cell expansion

Contact Info

Product

Resources

About