Effects of curcumin nanoparticle on the histological changes and apoptotic factors expression in testis tissue after methylphenidate administration in rats

Raoofi, Amir; Delbari, Ali; Mahdian, Davood; Mojadadi, Mohammad-Shafi; Akhlaghi, Maedeh; Dadashizadeh, Ghazaleh; Ebrahimi, Vahid; Amini, Abdollah; Golmohammadi, Rahim; Javadinia, Sara Sadat; Khaneghah, Amin Mousavi

doi:10.1016/j.acthis.2020.151656

Cited by 4 publications

(3 citation statements)

References 44 publications

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“…The results were analyzed using StepOne software (version 2.1; Applied Biosystems). The expression level of each target gene was normalized to its internal control ( β‐actin ), and the final analyses were performed using the comparative CT method (2 −ΔΔct ) (Raoofi et al, 2021 )…”

Section: Methodsmentioning

confidence: 99%

Stereological and molecular studies on the effects of Ferula persica extract on wound healing in rats

Huang¹,

Wang²,

Ebrahimzadeh

et al. 2021

Veterinary Medicine & Sci

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Background: Ferula persica is one of the most important traditional medicinal plants that is used to treat various diseases such as diabetes, backache and rheumatism. The aim of the present study was to evaluate the anti-inflammatory and wound healing potential of F. persica using stereological and molecular methods in experimental models. Methods:In the present study, two wound models (circular excision and linear incision) were used. Male Wistar rats were divided into four groups (n = 16), including control, vehicle treated, treated with F. persica extract ointment (5% w/w) and treated with the reference drug (Madecassol). All the animals were treated topically once a day. The circular and linear wounds were treated for 9 and 17 days, respectively. At the end of the study, samples from wounds area were harvested for histology, stereology, immunohistochemistry and molecular assessments to determine the in vivo healing potential and anti-inflammatory activity. Results:We observed significant recovery in macroscopic evaluation of wound healing in the F. persica extract treated group compared with the control and vehicle treated groups (p < 0.05). Histological and stereological assessments showed complete repair of the epidermal layer, increasing fibroblast cells and collagen density, decreasing inflammatory cells and a remarkable degree of neovascularization by determining length density of blood vessels in the extract group, which were significant as compared to the control and vehicle treated groups (p < 0.05). Expressions of TNF-α and TGF-β were found to be decreased and increased (p > 0.05, p < 0.05, respectively), in the extract treated group as compared to the control and vehicle treated groups. Also, greater COX-2 expression could be detected in the control and vehicle treated groups, which was significantly attenuated in the extract group. Conclusion:Our results confirm that the F. persica extract is a valuable source of antioxidant and anti-inflammatory activity and can allow damaged tissue in wounds to recover markedly.

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Section: Methodsmentioning

confidence: 99%

Stereological and molecular studies on the effects of Ferula persica extract on wound healing in rats

Huang¹,

Wang²,

Ebrahimzadeh

et al. 2021

Veterinary Medicine & Sci

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“…The Johnsen test was employed for histological evaluation of the tissue structure [33]. Stereological evaluations included the total volume of testicular tissue, the length density of seminiferous tubules, and the numerical densities of various cells, including spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and Leydig cells [36,37]. To evaluate the tissue structure and total volume, 5-μm-thick sections were prepared, while for the other measurements, 20-μm-thick sections were used.…”

Section: Histological and Stereological Evaluationmentioning

confidence: 99%

Caffeine attenuates spermatogenic disorders in mice with induced chronic scrotal hyperthermia

Raoofi,

Gholami,

Mokhtari

et al. 2024

Clin Exp Reprod Med

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Objective: Chronic scrotal hyperthermia (SHT) can lead to serious disorders of the male reproductive system, with oxidative stress playing a key role in the onset of these dysfunctions. Thus, we evaluated the impact of caffeine, a potent antioxidant, on cellular and tissue disorders in mice with chronic SHT.Methods: In this experimental study, 56 adult male NMRI mice were allocated into seven equal groups. Apart from the non-treated control group, all were exposed to heat stress. Two groups, termed “preventive” and “curative,” were orally administered caffeine. The preventive mice began receiving caffeine immediately prior to heat exposure, while for the curative group, a caffeine regimen was initiated 15 consecutive days following cessation of heat exposure. Each treated group was subdivided based on pairing with a positive control (Pre/curative [Cur]+PC) or a vehicle (Pre/Cur+vehicle). Upon conclusion of the study, we assessed sperm characteristics, testosterone levels, stereological parameters, apoptosis, antioxidant and oxidant levels, and molecular markers.Results: Sperm parameters, testosterone levels, stereological parameters, biochemical factors (excluding malondialdehyde [MDA]), and c-kit gene expression were significantly elevated in the preventive and curative groups, especially the former, relative to the other groups. Conversely, expression levels of the heat shock protein 72 (HSP72) and nuclear factor kappa beta (NF-κβ) genes, MDA levels, and apoptotic cell density were markedly lower in both caffeine-treated groups relative to the other groups, with more pronounced differences observed in the preventive group. Conclusion: Overall, caffeine attenuated cellular and molecular abnormalities induced by heat stress in the testis, particularly in the mice treated under the preventive condition.

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“…Restoring male fertility by cryopreservation of prepubertal testicular tissue ( Vermeulen et al, 2017 ) and post-tumor in vitro removal of tumor cells from spermatogonial cells for transplantation in testis after cancer treatment ( Shabani et al, 2018 ) have both met with some success. Amelioration of negative impacts of Ritalin (methylphenidate), commonly used for attention deficit hyperactivity disorder, on fertility by concurrent delivery of SPIONs carrying curcumin was assessed by Raoofi et al (2020) . Curcumin SPIONs were administered concurrently with methylphenidate to rats.…”

Section: Applications Of Nanoparticles In Male Reproductive Health Medicine and Researchmentioning

confidence: 99%

Biocompatible Nanomaterials as an Emerging Technology in Reproductive Health; a Focus on the Male

et al. 2021

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A growing body of research has confirmed that nanoparticle (NP) systems can enhance delivery of therapeutic and imaging agents as well as prevent potentially damaging systemic exposure to these agents by modifying the kinetics of their release. With a wide choice of NP materials possessing different properties and surface modification options with unique targeting agents, bespoke nanosystems have been developed for applications varying from cancer therapeutics and genetic modification to cell imaging. Although there remain many challenges for the clinical application of nanoparticles, including toxicity within the reproductive system, some of these may be overcome with the recent development of biodegradable nanoparticles that offer increased biocompatibility. In recognition of this potential, this review seeks to present recent NP research with a focus on the exciting possibilities posed by the application of biocompatible nanomaterials within the fields of male reproductive medicine, health, and research.

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Effects of curcumin nanoparticle on the histological changes and apoptotic factors expression in testis tissue after methylphenidate administration in rats

Cited by 4 publications

References 44 publications

Stereological and molecular studies on the effects of Ferula persica extract on wound healing in rats

Stereological and molecular studies on the effects of Ferula persica extract on wound healing in rats

Caffeine attenuates spermatogenic disorders in mice with induced chronic scrotal hyperthermia

Biocompatible Nanomaterials as an Emerging Technology in Reproductive Health; a Focus on the Male

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