Effects of culture time, ovarian activity, cumulus cells and sera on the nuclear and cytoplasmic maturation of pig oocytes in vitro

Ocampo, Marlon B.; Ocampo, Lerma C.; Ryu, Il-Sun; Mori, Tadashi; Ueda, Junji; Kanagawa, Hiroshi

doi:10.1016/0378-4320(93)90072-y

Cited by 10 publications

(5 citation statements)

References 32 publications

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“…The results have shown that oocytes with or without cumulus cells and/or with even or uneven granulation of the ooplasm could undergo meiotic resumption and subsequent completion of 1 st meiosis better than denuded oocytes. These findings concur with the observations in other mammalian species (Fukui et al, 1980;De Smedt et al, 1992;Ocampo et al, 1993). Apparently, the intercellular cooperation between the oocytes and cumulus cells via gap junctions is necessary for the growth of oocytes to proceed, most likely by taking up nutrients from the culture medium.…”

Section: Discussionsupporting

confidence: 82%

“…Cumulusoocyte complexes (COCs) were aspirated from antral follicles (3-5 mm in diameter) by using an 18 gauge needle attached to a 10 ml sterile plastic syringe, washed three times in the maturation medium before selecting using a stereomicroscope (Ocampo et al, 1993). A group of 10-15 COCs were transferred into 50µl droplets of maturation medium under mineral oil (Sigma Chem Co., St. Louis, USA; Lot No.…”

Section: Oocyte Collection and Maturationmentioning

confidence: 99%

“…The eggs were mounted, fixed for 24 hrs or more in 25% (v/v) aceto/ethanol at room temperature, stained with 1% (w/v) orcein in 45% (v/v) acetic acid and examined under phase-contrast microscope at 200-400x magnification. The meiotic stage of the oocytes and the nuclear stage after penetration were assessed according to Ocampo et al (1993). Oocytes with 2 nd metaphase plate and a polar body were regarded as matured (completed 1 st meiosis).…”

Section: Analysis Of the Nuclear Statusmentioning

confidence: 99%

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Factors influencing the maturation, fertilization and development of swamp buffalo oocyte in vitro

Ocampo¹,

Rigos²,

Ocampo³

2016

Res. Opin. Anim. Vet. Sci.

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Buffalo ovaries (n=610) were collected from the abattoir and the oocytes recovered were matured, fertilized and the resulting zygotes were cultured in vitro. In Experiment 1, the maturation and fertilization rates of O 1 -O 2 oocytes were higher than O 3 -O 5 oocytes. In Experiment 2, the two batches of TCM-199 medium used with different hormonal supplementation did not influence the maturation and fertilization of the oocytes at 70.7-86.7% and 59.4-66.0% respectively. In Experiment 3, bull variability was observed in terms of their capacity for fertilization. In Experiment 4, higher fertilization rate was observed in response to caffeine (3-10 mg/ml) compared to control. In Experiment 5, 1 × 10 6 sperm/ml concentration during fertilization had a lower penetration rate (43.0%) than 5, 10 × 10 6 sperm/ml concentration (68.4-71.0%). In Experiment 6, development to the blastocyst stage in TCM-199 or mSOF medium with or without cumulus cell monolayer had no significant difference (11.5-22.2%) with total cell count ranging from 84.6±11.2 to 91.4±12.2. Overall, the results showed the importance of cumulus cells in the acquisition of developmental competence of oocytes maturing in vitro and its subsequent fertilization. That, selection of appropriate bull, its optimum sperm concentration, the choice of capacitating agent and its concentration, the type of medium for culture of resulting zygotes is critical for successful application of in vitro maturation, fertilization and culturing for mass production of buffalo embryos. Keywords: Embryos; fertilization; maturation; oocytes To cite this article: Ocampo LC, LM Rigos and MB Ocampo, 2015. Factors influencing the maturation, fertilization and development of swamp buffalo oocyte in vitro. Res. Opin. Anim. Vet. Sci., 6(1): 24-31.

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Section: Discussionsupporting

confidence: 82%

Section: Oocyte Collection and Maturationmentioning

confidence: 99%

Section: Analysis Of the Nuclear Statusmentioning

confidence: 99%

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Factors influencing the maturation, fertilization and development of swamp buffalo oocyte in vitro

Ocampo¹,

Rigos²,

Ocampo³

2016

Res. Opin. Anim. Vet. Sci.

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“…The first study on IVM and IVF of dog oocytes was over 30 years ago by Mahi and Yanagimachi [14]. Since that report, and despite numerous studies documenting and focusing on establishing a suitable system for IVM of canine oocytes [15][16][17][18][19], the efficiency is lower than for other domestic mammalian species, for example cattle [20,21], sheep [22], pigs [23], mice [24], and cats [25]. Although puppies have been produced from somatic cell nuclear transfer of in-vivo matured oocytes [26], there has been no report of the production of live young after transfer of in-vitro maturation and fertilization-derived embryos [27].…”

Section: Introductionmentioning

confidence: 99%

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Abdel-Ghani

Abe

Asano

et al. 2010

Reprod Medicine & Biology

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Purpose To investigate the ability of medium conditioned with bovine cumulus-oocyte complexes (COCs) to support nuclear maturation of canine oocytes recovered from domestic dog ovaries. Methods Cumulus-oocyte complexes were obtained from ovaries of domestic bitches (8 months old to 7 years old), and in-vitro maturation was evaluated in TCM-199 supplemented with different concentrations (0, 20, 30 or 50%) of bovine COCs-conditioned medium (BCM). The canine COCs were cultured for 72 or 96 h at 38.5°C in 5% CO 2 , 5% O 2 and 90% N 2 . The bovine COCs-conditioned medium was obtained from culture of bovine COCs with TCM-199 supplemented with 5% FCS for 22 h at 38.5°C in 2% CO 2 , 98% air. Results The proportion of germinal vesicle breakdown (GVBD) after 72 h was significantly higher (P \ 0.05) in medium supplemented with 30% BCM (20.7%) compared with the control group (13.4%). The rates of GVBD-MII stage were significantly higher (P \ 0.05) when oocytes were matured with BCM at concentration of 30% (41.5%) compared with control (26.6%) after 72 h in-vitro culture. After 96 h in-vitro culture, the oocytes matured in medium supplemented with 30% BCM (5.5%) showed a significant increase (P \ 0.05) in the proportion of MII compared with control (0.7%). However, increasing the cultivation time from 72 to 96 h resulted in an increase in oocyte degeneration rate. Conclusions The results suggested that bovine COCsconditioned medium supplementation significantly increased nuclear maturation of canine oocytes.

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“…Hoy en día, la FIV se aplica de forma rutinaria en casi todas especies de mamíferos, para valorar la capacidad fecundante de los espermatozoides, lo que permite clasificar y seleccionar a los mejores reproductores en función de la calidad de su material germinal (Baker y Polge, 1976;Rath, 1991;Ocampo et al, 1994;Töpfer-Peterson, 1996;Córdova, 1994;Córdova et al, 1997;Roca et al, 1997;Telfer, 1998;Córdova, 1999;Córdova et al, 2001). …”

unclassified

Biotecnologías de reproducción animal con posibilidad de aplicación para optimizar el potencial reproductivo y productivo de los animales. Una revisión

Izquierdo

Lang

Campos

et al. 2011

Revista Complutense De Ciencias Veterinarias

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Effects of culture time, ovarian activity, cumulus cells and sera on the nuclear and cytoplasmic maturation of pig oocytes in vitro

Cited by 10 publications

References 32 publications

Factors influencing the maturation, fertilization and development of swamp buffalo oocyte in vitro

Factors influencing the maturation, fertilization and development of swamp buffalo oocyte in vitro

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Biotecnologías de reproducción animal con posibilidad de aplicación para optimizar el potencial reproductivo y productivo de los animales. Una revisión

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