Effects of culture medium strength and antioxidants on adventitious bud multiplication, hyperhydricity and tissue browning of date palm cv. Aziza Bouzid

Abstract

The effects of medium strength as well as different antioxidant types and concentrations on adventitious shoot bud multiplication, hyperhydricity and tissue browning of date palm cv. Aziza Bouzid were evaluated. Organogenic cultures of date palm cv. Aziza Bouzid were cultured on semi-solid Murashige and Skoog medium at half-strength (MS/2) or onethird-strength (MS/3), supplemented with 0.5 mg/L 2-naphthoxyacetic acid (NOA), 0.5 mg/L kinetin, and different concentrations of activated charcoal and polyvinylpyrrolidone (PVP). The findings of the present study showed that the use MS/3 media resulted in lower hyperhydricity rates than MS/2 media. Besides, activated charcoal significantly reduced tissue browning. The lowest rates of hyperhydricity (35%) and tissue browning (20%) were observed in MS/3 medium supplemented with 1.5 g/L activated charcoal. Interestingly, this culture medium showed also the highest number of adventitious shoot buds per explant (35.20), with a significant difference with all the other media (25.55-30.15 adventitious shoot buds per explant). Increasing the concentration of activated charcoal to 2 g/L did not decreased the browning rate. The use of PVP alone showed tissue browning rates varying from 30 to 55% while combining PVP and activated charcoal showed browning percentages ranging from 20 to 30%. Shoot elongation and rooting were successfully achieved and plantlet were acclimatized and showed normal growth and development under the greenhouse conditions.

Transformation of DHN1 gene and DHN promoter constructs into sugarcane calli, regeneration of the calli, and acclimatization of the plantlets

“…Some studies mentioned that reducing MS media strength may reduce osmosis levels and improve the acclimatization process in some plants i.e., Typhonium flagelliforme (Rezali et al, 2017), date palm cv. Aziza Bouzid (Elmaataoui et al, 2020), and apple (Malus domestica Borkh) 'Golden Delicious', 'Maksat', and 'Voskhod' (Kabylbekova et al, 2020). Nine out of a total of 25 tubes or clusters (Table 1) of both transgenic and control (wild type) acclimatization-ready sugarcane plantlets died after the acclimatization process.…”

Synergistic Effect of Plant Growth Regulators on Micropropagation of Eclipta alba: A Plant with Diverse Medicinal Properties

“…Furthermore, it has been shown that plants grown in vitro conditions occasionally experience nutritional stress and exhibit restricted root system growth to maximize nutrient absorption. Therefore, it's important to maximize nutrient concentrations since they frequently affect root growth by activating local and systemic signaling pathways (Elmaataoui et al 2020). Therefore the impact of medium strength (full strength MS, ½ MS, and ¼ MS) on the rooting efficiency of E. alba microshoots was also investigated (Table 3).…”