Effects of culture conditions on multiple shoot induction from inflorescence and RAPD analysis of cloned plants in Limonium sinensis (Girard) Kuntze, var. Golden Diamond

Dam, Anandamoy; Paul, Santanu; Bhattacharya, Chayanika; Bandyopadhyay, Tapas Kumar

doi:10.1007/s13562-012-0164-8

Cited by 11 publications

(4 citation statements)

References 13 publications

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“…All of the experiments were carried out in a randomized block design and each set of experiment comprised of ten explants or cultures in triplicate. The results were analyzed as mean ± standard errors (SE) and the percentage data were arcsine-transformed following the method of our previous study [ 28 ], [ 29 ]. The significant difference ( p ≤ 0.05) among the mean was assessed by analysis of variance followed by Duncan’s multiple range test (DMRT) using MSTAT-C software (ver.…”

Section: Methodsmentioning

confidence: 99%

Regeneration of plantlets through somatic embryogenesis from root derived calli of Hibiscus sabdariffa L. (Roselle) and assessment of genetic stability by flow cytometry and ISSR analysis

et al. 2018

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Induction of somatic embryogenesis and complete plantlet regeneration from callus culture of Hibiscus sabdariffa L. var. HS4288 has been made. Leaf and root explants were cultured on Murashige and Skoog (MS) and Driver–Kuniyuki Walnut (DKW) basal media supplemented with different concentrations of synthetic auxins and cytokinins. Root explants on DKW medium supplemented with 2.26μM 2, 4-Dichlorophenoxyacetic acid (2, 4-D) and 4.65μM kinetin (KIN) induced highest percentage (70%) of embryogenic calli. Average number of globular embryos per root derived callus produced within 6 weeks of culture initiation on MS media with different plant growth regulators (PGRs) ranged from 2.27±0.12 to 8.80±0.17 and that of cotyledonary embryos ranged from 0.00 to 2.53±0.20. On DKW medium comparatively more globular embryos (2.70±0.15 to 14.53±0.23) and cotyledonary embryos (0.00 to 8.90±0.17) were produced than that of MS medium. Regeneration of complete plantlets was highest (76.67%) when embryogenic calli with mature somatic embryos were grown on DKW medium containing 2.32μM KIN and 2.22μM 6-Benzyladenine (BA). Plants were primarily hardened in humidity, temperature and light controlled chamber and finally in a greenhouse showed 70% survival ability. Different stages of somatic embryogenesis process in the root derived embryogenic calli were elaborated in detail by morphological, histological and SEM study. The data were statistically analyzed by Duncan Multiple range test (p ≤ 0.05) and Principal component analysis (PCA). Flow cytometry and Inter-simple sequence repeats (ISSR) marker analysis confirmed that there was no genetic variation within the regenerated plants.

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Section: Methodsmentioning

confidence: 99%

Regeneration of plantlets through somatic embryogenesis from root derived calli of Hibiscus sabdariffa L. (Roselle) and assessment of genetic stability by flow cytometry and ISSR analysis

et al. 2018

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“…RAPD has been one of the most commonly used methods because it is simple, rapid and cost-effective. It has proven to be a suitable molecular technique to detect genetic variation that occurs during in vitro regeneration of different plant species such as grape (Yang et al, 2008), cotton (Jin et al, 2008), potato (Vargas et al, 2008), chrysanthemum (Miñano et al, 2009), date palm (Ahmed et al, 2009), olive (Peyvandi et al, 2009), cowpea (Sivakumar et al, 2011), jojoba (Kumar et al, 2011), Viola patrinii (Chalageri and Babu, 2012) and Limonium sinensis (Dam et al, 2013). Except for chromosome counting (Laublin et al, 1991;Laublin and Cappadocia, 1992;Radojević and Subotić, 1992), no studies have been performed to check the genetic stability and uniformity of Iris sp.…”

Section: Introductionmentioning

confidence: 99%

Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture — RAPD and flow cytometer analyses

Stanišić

Raspor

Ninković

et al. 2015

South African Journal of Botany

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Efficient protocols, safe from somaclonal variation, were developed for regeneration of Iris sibirica plants via organogenesis and somatic embryogenesis from leaf-base explants cultivated on Murashige and Skoog media supplemented with thidiazuron (TDZ, 1.0 mg/l) or 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/l). The morphogenic response and callus formation efficiency differed significantly between 2,4-D (80.9%) and TDZ (67%) morphogenesis induction treatments. TDZ induced only organogenic calli, while calli obtained with 2,4-D were composed of three types differing in color and consistency: white, friable -embryogenic calli (4.5%, 3.8 mg/explant), green, compact -organogenic calli (12.4%, 48.4 mg/explants) and yellow -nonregenerative calli (77.3%, 254.4 mg/explant). The cultivation of embryogenic calli on medium with 2,4-D and Kinetin resulted in further development of somatic embryos (54 embryos/g of calli) which germinated with a frequency of 62% after being transferred to a medium without plant growth regulators. Stable shoot cultures were established by transferring organogenic calli with shoot primordia to media with 0.1 mg/l α-naphthaleneacetic acid (NAA) and 1.0 mg/l 6-benzylaminopurine (BA), while further cultivation on media of the same composition (TDZ or 2,4-D) resulted in the reduced growth and rhizogenesis, respectively. The TDZ induction treatment resulted in higher number of shoots per explant (7.9) than the 2,4-D treatment (4.3). After successful rooting and ex vitro acclimatization, plants were grown in the field and flowered to seed production. Flow cytometry, chromosome counting and random amplification of polymorphic DNA (RAPD) analysis indicated no evidence of genetic variation in plants regenerated via somatic embryogenesis or organogenesis. The results suggest that established protocols are safe for use in genetic transformation procedures or large-scale production of true-to-type I. sibirica plants.

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“…All plants were analyzed by 25 RAPD primers out of which 15 RAPD primers responded as mentioned in Table 3. PCR amplification was performed in a thermal cycler (Applied Biosystems, 2720 Thermal Cycler, Foster City, CA 94404 USA) in a solution suggested by Dam et al (2013). The amplification program was set at an initial denaturation of 94°C for 5 min followed by 45 cycles of 1 min denaturation at 94°C, 30 s annealing at 35°C, and 2 min extension at 72°C with a final extension at 72°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

Bhattacharya

Dam

Karmakar

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

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We present here a method for the in vitro propagation of Anthurium andraeanum cv. Fantasia through direct somatic embryogenesis. The embryos were directly formed at the cut end of most of the leaf explants when cultured on MS basal medium supplemented with N 6 -benzyladenine (BA) plus α-naphthalene acetic acid (NAA). MS basal media supplemented with BA (0.27 μM) and NAA (2.68 μM) induced maximum number of somatic embryos per leaf explant (15.33 ± 1.45) after 28 d of continuous culture. The highest numbers of embryos (12.33 ± 0.88) were matured into plantlets in MS basal medium augmented with 0.27 μM BA and 58.4 mM sucrose. Histology showed the presence of scutellum, coleoptile, welldeveloped root, and shoot initials at different stages of somatic embryo (SE) development directly on leaf explants. About 85% of the plantlets were successfully acclimatized, and of these, 80.3% plants survived after secondary hardening under greenhouse conditions. The embryo-derived plants successfully flowered. The presence of monomorphic DNA bands in random amplified polymorphic DNA (RAPD) marker analysis confirmed the genetic homogeneity of direct somatic embryo-derived plantlets in respect to their mother plant.

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Effects of culture conditions on multiple shoot induction from inflorescence and RAPD analysis of cloned plants in Limonium sinensis (Girard) Kuntze, var. Golden Diamond

Cited by 11 publications

References 13 publications

Regeneration of plantlets through somatic embryogenesis from root derived calli of Hibiscus sabdariffa L. (Roselle) and assessment of genetic stability by flow cytometry and ISSR analysis

Regeneration of plantlets through somatic embryogenesis from root derived calli of Hibiscus sabdariffa L. (Roselle) and assessment of genetic stability by flow cytometry and ISSR analysis

Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture — RAPD and flow cytometer analyses

Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

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