Effects of culture conditions on the production of polyhydroxyalkanoates by Azotobacter chroococcum H23 in media containing a high concentration of alpechı́n (wastewater from olive oil mills) as primary carbon source

Pozo, Clementina; Martı́nez-Toledo, M.V.; Rodelas, B.; González‐López, Jesús

doi:10.1016/s0168-1656(02)00056-1

Cited by 75 publications

(46 citation statements)

References 16 publications

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“…Pozo et al Growth and production of PHA in nutrient broth and preprocessed cardboard industry waste water after 72 h of incubation at 37°C. [40] found that NH 4 medium amended with 60% alpechin showed the maximum PHA production and concluded that alpechin was tolerated by A. chroococcum strain H23 at high concentrations and that it acts as a substrate to support the growth of the microorganisms.…”

Section: Growth Pattern and Phb Production In Cardboard Industry Wastmentioning

confidence: 99%

Poly-β-hydroxybutyrate production and management of cardboard industry effluent by new Bacillus sp. NA10

Bhuwal

Singh

Aggarwal

et al. 2014

Bioresour. Bioprocess.

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Background: In the present study, we aim to utilize the ecological diversity of soil for the isolation and screening for poly β-hydroxybutyrate (PHB)-accumulating bacteria and production of cost-effective bioplastic using cardboard industry effluent. Results: A total of 120 isolates were isolated from different soil samples and a total of 62 isolates showed positive results with Nile blue A staining, a specific dye for PHB granules and 27 isolates produced PHB using cardboard industry effluent. The selected isolate NA10 was identified as Bacillus sp. NA10 by studying its morphological, biochemical, and molecular characteristics. The growth pattern for the microorganism was studied by logistic model and exactly fitted in the model. A maximum cell dry weight (CDW) of 7.8 g l −1 with a PHB concentration of 5.202 g l −1 was obtained when batch cultivation was conducted at 37°C for 72 h, and the PHB content was up to 66.6% and productivity was 0.072 g l −1 h −1 in 2.0 L fermentor. Chemical characterization of the extracted PHB was done by H 1 NMR, Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), Gas chromatography-mass spectrometry (GC-MS) analysis to determine the structure, melting point, and molecular mass of the purified PHB. The polymer sheet of extracted polymer was prepared by blending the polymer with starch for packaging applications. Conclusions: The isolate NA10 can be a good candidate for industrial production of PHB from cardboard industry waste water cost-effectively and ecofriendly.

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Section: Growth Pattern and Phb Production In Cardboard Industry Wastmentioning

confidence: 99%

Poly-β-hydroxybutyrate production and management of cardboard industry effluent by new Bacillus sp. NA10

Bhuwal

Singh

Aggarwal

et al. 2014

Bioresour. Bioprocess.

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“…It has been reported that OMWW can also be used as a fermentation substrate to obtain other types of microbial polymers, such as homo-and co-polymers of polyhydroxyalkanoates (PHAs; Martinez-Toledo et al 1995;Gonzalez-Lopez et al 1996;Pozo et al 2002). These substances are accumulated as intracellular granules in a variety of bacteria and are a source of new biodegradable plastics.…”

Section: Biopolymers and Enzymesmentioning

confidence: 99%

“…These substances are accumulated as intracellular granules in a variety of bacteria and are a source of new biodegradable plastics. The production of PHA by Azotobacter chroococcum strain H23 in a medium prepared with OMWW (diluted up to 60% with water) as the only source of carbon was increased by aeration of the cultures and the addition of nitrogen (0.12% ammonium acetate w/v), with a maximal yield of 6.2 g PHA per litre culture medium (Pozo et al 2002). The production of enzymes by fungi using OMWs as substrate with commercial interest offers another interesting opportunity for the biotechnological valorisation of the residues.…”

Section: Biopolymers and Enzymesmentioning

confidence: 99%

Bioremediation and biovalorisation of olive-mill wastes

Morillo

Antízar-Ladislao

Monteoliva‐Sánchez

et al. 2009

Appl Microbiol Biotechnol

199

104

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Olive-mill wastes are produced by the industry of olive oil production, which is a very important economic activity, particularly for Spain, Italy and Greece, leading to a large environmental problem of current concern in the Mediterranean basin. There is as yet no accepted treatment method for all the wastes generated during olive oil production, mainly due to technical and economical limitations but also the scattered nature of olive mills across the Mediterranean basin. The production of virgin olive oil is expanding worldwide, which will lead to even larger amounts of olive-mill waste, unless new treatment and valorisation technologies are devised. These are encouraged by the trend of current environmental policies, which favour protocols that include valorisation of the waste. This makes biological treatments of particular interest. Thus, research into different biodegradation options for olive-mill wastes and the development of new bioremediation technologies and/or strategies, as well as the valorisation of microbial biotechnology, are all currently needed. This review, whilst presenting a general overview, focusses critically on the most significant recent advances in the various types of biological treatments, the bioremediation technology most commonly applied and the valorisation options, which together will form the pillar for future developments within this field.

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“…Finding a less expensive substrate is, therefore, a major need for a wide commercialisation of these products. Large amounts of biopolymers containing β-hydroxybutyrate (PHB) and copolymers containing β-hydroxyvalerate (P[HB-co-HV]) are produced by Azotobacter chroococcum in culture media amended with alpechin (wastewater from olive oil mills) as the sole carbon source (Pozo et al 2002).…”

Section: Biopolymers and Biodegradable Plasticsmentioning

confidence: 99%

Microbial Biotechnology in Olive Oil Industry

Darvishi¹

2012

Olive Oil - Constituents, Quality, Health Properties and Bioconversions

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Effects of culture conditions on the production of polyhydroxyalkanoates by Azotobacter chroococcum H23 in media containing a high concentration of alpechı́n (wastewater from olive oil mills) as primary carbon source

Cited by 75 publications

References 16 publications

Poly-β-hydroxybutyrate production and management of cardboard industry effluent by new Bacillus sp. NA10

Poly-β-hydroxybutyrate production and management of cardboard industry effluent by new Bacillus sp. NA10

Bioremediation and biovalorisation of olive-mill wastes

Microbial Biotechnology in Olive Oil Industry

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