Effects of Culture Condensate of Bifidobacterium longum (MB) on Feed Efficiency, Morphology of Intestinal Epithelial Cells, and Fecal Microflora of Rats
“…Mice were fed ad libitum with a basal pelleted diet supplemented with 5% (w/w) powdered culture condensate of B. longum or L. acidophilus. The culture condensates of B. longum and L. acidophilus were prepared as described by Fujisawa et al,11) harvested by centrifugation and then washed three times with saline. After adjusting the bacterial concentration to more than 4 x 1011 CFU/ml, the culture condensates were heated to 80°C and then lyophilized to a powder containing about 8 x lOll nonviable cells/g.…”
Section: Preparation Of Bacterial Cells and Cell Fractions B Longummentioning
“…Mice were fed ad libitum with a basal pelleted diet supplemented with 5% (w/w) powdered culture condensate of B. longum or L. acidophilus. The culture condensates of B. longum and L. acidophilus were prepared as described by Fujisawa et al,11) harvested by centrifugation and then washed three times with saline. After adjusting the bacterial concentration to more than 4 x 1011 CFU/ml, the culture condensates were heated to 80°C and then lyophilized to a powder containing about 8 x lOll nonviable cells/g.…”
Section: Preparation Of Bacterial Cells and Cell Fractions B Longummentioning
“…It is well known that functional foods control the metabolism of intestinal bacteria and activate the non-specific immune function through improvement of the intestinal environment [4]. AP reduced the β-glucuronidase activities and the secondary bile acids concentration [6], and MB controlled the intestinal flora [1] and stimulated the immune function [9]. Tg male and female CB6F1-Tg H ras2 mice carrying human c-Ha-ras genes and non-transgenic (non-Tg) littermates of the same background [8] were used.…”
Abstract:The number and tumor score of colorectal tumors induced by 1,2-dymethylhydrazine in transgenic (Tg) mice carrying human c-Ha-ras genes were significantly reduced by ingestion of apple pectin (AP) or a culture condensate of Bifidobacterium longum (MB) when compared with a control diet. There was no statistical difference in the incidence of colorectal tumors in Tg mice between the AP or MB diet and the control diet. This study demonstrated that Tg mice are a useful tool for screening inhibition of colorectal tumors by functional foods. Key wards: c-Ha-ras gene, colorectal tumor, transgenic mice mors induced by 1,2-dimethylhydrazine injection. It is well known that functional foods control the metabolism of intestinal bacteria and activate the non-specific immune function through improvement of the intestinal environment [4]. AP reduced the β-glucuronidase activities and the secondary bile acids concentration [6], and MB controlled the intestinal flora [1] and stimulated the immune function [9]. Tg male and female CB6F1-Tg H ras2 mice carrying human c-Ha-ras genes and non-transgenic (non-Tg) littermates of the same background [8] were used. These mice were fed the Japan Transgenic (Tg) mice carrying human prototype cHa-ras genes are useful animal models of human cancers [3] and show high sensitivity to genotoxic carcinogens [11]. We reported that colorectal tumors induced by 1,2-dimethylhydrazine (DMH) in Tg mice were modified by the combination of intestinal bacteria and c-Ha-ras genes [5]. These data suggested that the intestinal environment may control colorectal tumors in Tg mice. In this study, we fed Tg mice apple pectin (AP) or a culture condensate of Bifidobacterium longum (MB), and examined the suppression of colorectal tu-
“…Although MB diet affected the microbial composition and physiological conditions of intestinal epithelial cells [11,16], the relationship between these alterations of the epithelial cells after MB ingestion and BTL inhibition is not clear.…”
Section: Discussionmentioning
confidence: 99%
“…Viable counts after evaporation were less than the detection limit (1 × 10 4 cfu/ml). The composition of MB was 19.2% protein, 10.9% lactose, 6.4% acetic acid, 6.5% lactic acid, 12.8% ash, 6.5% galactose, 6.0% others, and 31.7% water [11].…”
mentioning
confidence: 99%
“…It has also been reported that treatment with Bifidobacterium stimulates the nonspecific immune function [23,27], and Fujisawa et al [11] have reported that an administration of a culture condensate of Bifidobacterium longum (MB) increased the enzyme activity of intestinal epithelial cells and controlled the intestinal flora in rats.…”
ABSTRACT. Bacterial translocation occurs when viable bacteria pass through the mucosa of the gastrointestinal tract to the mesenteric lymph nodes (MLN) and other organs. The ability of a diet containing 1% culture condensate of Bifidobacterium longum (MB) to inhibit bacterial translocation from the gastrointestinal tract was tested using antibiotic-decontaminated specific-pathogen-free (SPF) mice and germ-free mice, both of which were monoassociated with Escherichia coli C25. Feeding of MB diet decreased the number of E.coli C25 translocating to MLN to about half the number of the control diet group, but did not reduce the incidence of translocation to MLN. MB diet ingestion also decreased the number of E.coli C25 translocating to MLN in the SPF mice injected with zymosan, but it could not prevent bacteria translocation in mice receiving a 30 % thermal injury. -KEY WORDS : bacterial translocation, Bifidobacterium, mouse, thermal injury, zymosan.
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