Effects of cryoprotectants on actin filaments during the cryopreservation of one-cell rabbit embryos

Vincent, Caroline; Prulière, Gérard; Pajot‐Augy, Edith; Campion, Evelyne; Garnier, V.; Renard, Jean‐Paul

doi:10.1016/0011-2240(90)90048-9

Cited by 51 publications

(19 citation statements)

References 52 publications

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“…It has been reported that there is no increase in the rates of aneuploidy or abnormal karyotype in onecell rabbit embryos [64] and mature mouse or human oocytes [65,66] when they were treated with PROH as a cryoprotectant. However, PROH is not effective for cryopreservation of mouse and human oocytes when a slow freezing procedure is u s e d b e c a u s e o f a h i g h i n c i d e n c e o f parthenogenetic activation found after freezingthawing with 1.5 M of PROH [60,67,68].…”

Section: Discussionmentioning

confidence: 99%

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

Chian

Kuwayama

Tan

et al. 2004

Journal of Reproduction and Development

165

100

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Abstract. Improving pregnancy rates associated with the use of cryopreserved human oocytes would be an important advance in human assisted reproductive technology (ART). Vitrification allows glasslike solidification of a solution without ice crystal formation in the living cells. We have attempted to improve the survival rates of oocytes by a vitrification technique using bovine models. In vitro matured oocytes with or without cumulus cells were vitrified with either 15.0% (v/v) ethylene glycol (EG) + 15% (v/v) dimethylsulfoxide (DMSO) + 0.5 M sucrose or 15% (v/v) EG + 15% (v/v) 1,2-propanediol (PROH) + 0.5 M sucrose, using 'Cryotop' or 'thin plastic sticker', respectively. The oocyte survival rates after vitrifying-warming, and the capacity for fertilization and embryonic development were examined in vitro. The rate of embryonic development to blastocyst was significantly higher (P<0.05) in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) PROH + 0.5 M sucrose than in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) DMSO + 0.5 M sucrose (7.4% ± 4.1 vs. 1.7% ± 3.0, respectively). Oocytes vitrified without cumulus cells had a higher survival rate after thawing and a superior embryonic developmental capacity compared with oocytes vitrified with cumulus cells. Prolonged pre-incubation time after thawing adversely affected the rates of embryonic cleavage and development. These results indicate that in vitro matured bovine oocytes can be vitrified successfully with the mixture of the cryoprotectants, EG + PROH, the absence of cumulus cells for vitrification does not affect oocyte survival rate after warming, and vitrified and warmed oocytes do not require pre-incubation before in vitro fertilization.

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Section: Discussionmentioning

confidence: 99%

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

Chian

Kuwayama

Tan

et al. 2004

Journal of Reproduction and Development

165

100

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“…Previous studies of the action of cryosolvents on F-actin have indicated two effects of propanediol: 1) in the presence of 2 to 6 M propanediol, shortening and bundling of actin microfflaments is observed (Vincent et al 1987(Vincent et al , 1990Nguyen 1991). Glycerol has no such effect.…”

Section: Discussionmentioning

confidence: 95%

“…Several observations have been made on the modifications undergone by F-actin in the presence of 1,2-propanediol. Among them are shortening, bundling of microfilaments (Vincent et al 1987(Vincent et al , 1990, and conformational change of actin protomers within the filaments which yields a conformation closer to that of monomeric actin (Nguyen 1991).…”

Section: Introductionmentioning

confidence: 98%

“…It could even induce vitrification of the cytoplasm upon cooling (Pruli&e et al 1987;Pruli&e and Douzou 1989). Therefore, a range of studies involving both in vivo and in vitro investigations was undertaken to examine the action of 1,2-propanediol, an efficient cryoprotectant for early mammal embryos (Vincent et al 1990;Renard et al 1984;Glenister et al 1990), on actin, which is a major cytoplasmic protein.…”

Section: Introductionmentioning

confidence: 99%

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The effect of organic cryosolvents on actin structure: studies by small angle X-ray scattering

Pajot‐Augy

Axelos

1992

Eur Biophys J

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Small-angle X-ray scattering was used to probe the structure of actin in the presence of cryosolvents: 1,2-propanediol, glycerol, or a mixture of both solvents. In media devoid of polymerizing salts, a radius of gyration of 23 A is measured, as expected from the literature. In the presence of 1,2-propanediol alone, the scattering pattern begins to exhibit the characteristic slope of elongated objects with a non-negligible thickness, such as actin filaments polymerized in 40 mM KCl and 1 mM MgCl2. However, only short fragments (radius of gyration 40 A) are generated. We infer that in a medium of low ionic strength containing 15% 1,2-propanediol, actin assumes a structure closer to that of filamentous actin. 1,2-propanediol apparently induces nucleation of oligomers, as with polymerizing salts, but no propagation occurs. Glycerol and/or propanediol induce no alteration in the structure of individual salt-polymerized actin filaments. Aggregation occurs with propanediol, even in the presence of glycerol. Glycerol alone has no such effect. No shortening is detected within the scale covered, with either solvent, although 1,2-propanediol is known to shorten actin filaments. We suggest that in the absence of salts, 1,2-propanediol induces a conformational change in monomeric actin that is necessary for nucleation. This could correlate with a conformational change of actin promoters within microfilaments observed in the presence of 1,2-propanediol by other authors using different techniques.

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“…Oocyte cryopreservation has been associated with intracellular organelles and cytoskeleton injuries, such as alteration in mitochondrial function [4], hardening [5] and fracturing [6] of the zona pellucida, microtubules damage [7,8], ultrastructural alterations in microdomains and meiotic spindle damage [9][10][11][12], which are presumably linked to the reduced developmental potential of cryopreserved oocytes [13]. However, protection of the meiotic spindle has been inferred by the addition of cryoprotectants [14] and by de novo spindle reconstruction following warming [15].…”

Section: Introductionmentioning

confidence: 99%

Oocyte morphology does not affect post-warming survival rate in an egg-cryobanking donation program

Setti

Figueira²,

Braga

et al. 2011

J Assist Reprod Genet

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Purpose To evaluate whether oocyte dysmorphisms affect oocyte survival rates in an egg-cryobanking donation program. Methods This study included 54 patients undergoing intracytoplasmic sperm injection. A total of 415 metaphase II oocytes were vitrified using the Cryotop method. Oocyte morphology was assessed immediately prior to oocyte vitrification under 400× magnification. The influence of dysmorphisms on post-thaw survival rates was assessed using regression analysis. Results were considered to be significant at the 5% critical level. Results Oocyte survival rate was not affected by the presence of the following analysed oocyte abnormalities: increased cytoplasmic granularity, vacuoles in the ooplasm, aggregates of smooth endoplasmic reticulum in the ooplasm, large perivitelline space size, perivitelline space granularity, fragmented first polar body and zona pellucida abnormalities. Conclusions Oocyte morphology, observed prior to vitrification, does not predict post-warming survival. The noninvasive identification of predictive markers for oocyte survival potential remains a difficult task.

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Effects of cryoprotectants on actin filaments during the cryopreservation of one-cell rabbit embryos

Cited by 51 publications

References 52 publications

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

The effect of organic cryosolvents on actin structure: studies by small angle X-ray scattering

Oocyte morphology does not affect post-warming survival rate in an egg-cryobanking donation program

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