Effects of creatine supplementation associated with resistance training on oxidative stress in different tissues of rats

Abstract

BackgroundCreatine supplementation is known to exert an effect by increasing strength in high intensity and short duration exercises. There is a hypothesis which suggests that creatine supplementation may provide antioxidant activity by scavenging Reactive Oxygen Species. However, the antioxidant effect of creatine supplementation associated with resistance training has not yet been described in the literature. Therefore, we investigated the effect of creatine monohydrate supplementation associated with resistance training over maximum strength gain and oxidative stress in rats.MethodsForty male Wistar rats (250-300 g, 90 days old) were randomly allocated into 4 groups: Sedentary (SED, n = 10), Sedentary + Creatine (SED-Cr, n = 10), Resistance Training (RT, n = 10) and Resistance Training + Creatine (RT-Cr, n = 10). Trained animals were submitted to the RT protocol (4 series of 10–12 repetitions, 90 second interval, 4 times per week, 65% to 75% of 1MR, for 8 weeks).ResultsIn this study, greater strength gain was observed in the SED-Cr, RT and RT-Cr groups compared to the SED group (P < 0.001). The RT-Cr group showed a higher maximum strength gain when compared to other groups (P < 0.001). Creatine supplementation associated with resistance training was able to reduce lipoperoxidation in the plasma (P < 0.05), the heart (P < 0.05), the liver (P < 0.05) and the gastrocnemius (P < 0.05) when compared to control groups. However, the supplementation had no influence on catalase activity (CAT) in the analyzed organs. Only in the heart was the CAT activity higher in the RT-Cr group (P < 0.05). The activity of superoxide dismutase (SOD) was lower in all of the analyzed organs in the SED-Cr group (P < 0.05), while SOD activity was lower in the trained group and sedentary supplemented group (P < 0.05).ConclusionsCreatine was shown to be an effective non-enzymatic antioxidant with supplementation alone and also when it was associated with resistance training in rats.

High-intensity Exercise Modifies the Effects of Stanozolol on Brain Oxidative Stress in Rats

“…Accordingly, in the study performed by Li and Wang [37], rats exhibited significant increases in brain CAT activity after the combination of running on a treadmill for 5 weeks (6 days per week with a gradual speed of 20-30 m/min for 30-60 min/day) with hypoxic conditions (3 500 m altitude), considering this as an HIE protocol. Furthermore, others [58] have reported that plasma CAT activity was higher after an HIE protocol consisted of 4 series of 10-12 repetitions and 90 s intervals, 4 times per week, 65-75 % of the one maximum repetition for 8 weeks. These assertions concur with our outcomes that point to an increased brain CAT activity, which might be produced as auto-defense by the type of HIE carried out under our experimental conditions that led to a higher production of ROS.…”

Efeitos Do Exercício Resistido De Alta Intensidade Em Ratos Que Receberam Dexametasona

“…De acordo com a literatura 33,34 , sujeitos com baixos níveis de aptidão física possuem a tendência em apresentar maior magnitude de adaptações morfofuncionais, essa observação é denominada como princípio da treinabilidade 34 . O modelo de treinamento utilizado foi o de agachamento, elaborado por TAMAKI e colaboradores 19 o qual tem sido aplicado em diversos trabalhos para mimetizar respostas semelhantes às obtidas em humanos adeptos ao exercício resistido [35][36][37] . Um dos primeiros efeitos adversos causados pela dexametasona foi a diminuição progressiva de massa corporal total 10,[12][13] .…”

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“…An electrical stimulus (4-5 mA , 0.3 seconds long, with a 3 second interval between each repetition) [10] was applied in the rat's tail using a surface electrode, in order to provoke the extension movement of the lower limbs of the rat.…”