Effects of CPAP therapy withdrawal on exhaled breath pattern in obstructive sleep apnoea

Schwarz, Esther I.; Sinués, Pablo Martínez-Lozano; Bregy, Lukas; Gaisl, Thomas; Gomez, Diego A.; Gaugg, Martin Thomas; Suter, Yannick; Stebler, Nina; Nussbaumer-Ochsner, Yvonne; Bloch, Konrad E.; Stradling, John; Zenobi, Renato; Kohler, Malcolm

doi:10.1136/thoraxjnl-2015-207597

Cited by 56 publications

(82 citation statements)

References 45 publications

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“…The methods used for sampling, processing, and analyzing the data in this study have been published in detail elsewhere (19 ). In short, study requirements for participants were to refrain from consumption of alcohol and caffeine or use of tobacco and chewing gum 1 hour before assessment.…”

Section: Real-time Breath Analysismentioning

confidence: 99%

“…All candidates were in a fasting state before measurement (19 ). For SESI-HRMS, a standardized protocol was applied to all participants to exclude the influence of breathing maneuvers on exhaled compounds and to keep artifacts to a minimum level (19,30,31 ). Exhalations via a mouthpiece at a level of 10 mbar lasting 15 s were repeated 6 times.…”

Section: Real-time Breath Analysismentioning

confidence: 99%

“…1). The last 6 s of each exhalation (approximately 15 s) were considered for analysis, thus excluding the initial dead volume (air from the upper airways which is inhaled and does not take part in the gas exchange) (19 ). Signals obtained for each amino acid, by integrating the HRMS baseline resolved peaks, were normalized against the signal of an internal standard, D 4 -alanine (integrated over the same 6 s).…”

Section: Real-time Breath Analysismentioning

confidence: 99%

“…In short, study requirements for participants were to refrain from consumption of alcohol and caffeine or use of tobacco and chewing gum 1 hour before assessment. All candidates were in a fasting state before measurement (19 ). For SESI-HRMS, a standardized protocol was applied to all participants to exclude the influence of breathing maneuvers on exhaled compounds and to keep artifacts to a minimum level (19,30,31 ).…”

Section: Real-time Breath Analysismentioning

confidence: 99%

“…Breath analysis can detect differences between individual "breathprints" which are reasonably stable over time (13 ), can be used to follow diurnal metabolic patterns (14,15 ), and is capable of quantifying hitherto unknown compounds in human breath (16,17 ). It also has been proposed for the detection of biomarkers to diagnose lung and sleep diseases (18,19 ). In all of these applications, breath analysis is usually thought of as being restricted to volatile or semivolatile compounds that cross from the blood to the breath via the alveolar compartment (20 ).…”

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confidence: 99%

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Real-Time Quantification of Amino Acids in the Exhalome by Secondary Electrospray Ionization–Mass Spectrometry: A Proof-of-Principle Study

et al. 2016

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BACKGROUND:Amino acids are frequently determined in clinical chemistry. However, current analysis methods are time-consuming, invasive, and suffer from artifacts during sampling, sample handling, and sample preparation. We hypothesized in this proof-of-principle study that plasma concentrations of amino acids can be estimated by measuring their concentrations in exhaled breath. A novel breath analysis technique described here allows such measurements to be carried out in real-time and noninvasively, which should facilitate efficient diagnostics and give insights into human physiology.

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Section: Real-time Breath Analysismentioning

confidence: 99%

Section: Real-time Breath Analysismentioning

confidence: 99%

Section: Real-time Breath Analysismentioning

confidence: 99%

Section: Real-time Breath Analysismentioning

confidence: 99%

mentioning

confidence: 99%

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Real-Time Quantification of Amino Acids in the Exhalome by Secondary Electrospray Ionization–Mass Spectrometry: A Proof-of-Principle Study

et al. 2016

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Direct mass spectrometry analysis of exhaled human breath in real‐time

Zhang,

Frankevich,

Ding

et al. 2023

Mass Spectrometry Reviews

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The molecular composition of exhaled human breath can reflect various physiological and pathological conditions. Considerable progress has been achieved over the past decade in real‐time analysis of exhaled human breath using direct mass spectrometry methods, including selected ion flow tube mass spectrometry, proton transfer reaction mass spectrometry, extractive electrospray ionization mass spectrometry, secondary electrospray ionization mass spectrometry, acetone‐assisted negative photoionization mass spectrometry, atmospheric pressure photoionization mass spectrometry, and low‐pressure photoionization mass spectrometry. Here, recent developments in direct mass spectrometry analysis of exhaled human breath are reviewed with regard to analytical performance (chemical sensitivity, selectivity, quantitative capabilities) and applications of the developed methods in disease diagnosis, targeted molecular detection, and real‐time metabolic monitoring.

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Alternative electrolyte solutions for untargeted breath metabolomics using secondary‐electrospray ionization high‐resolution mass spectrometry

Wüthrich,

Zenobi,

Giannoukos

2024

Rapid Comm Mass Spectrometry

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RationaleSecondary‐electrospray ionization (SESI) coupled with high‐resolution mass spectrometry is a powerful tool for the discovery of biomarkers in exhaled breath. A primary electrospray consisting of aqueous formic acid (FA) is currently used to charge the volatile organic compounds in breath. To investigate whether alternate electrospray compositions could enable different metabolite coverage and sensitivities, the electrospray dopants NaI and AgNO3 were tested.MethodsIn a proof‐of‐principle manner, the exhaled breath of one subject was analyzed repeatedly with different electrospray solutions and with the help of a spectral stitching technique. Capillary diameter and position were optimized to achieve proper detection of exhaled breath. The detected features were then compared using formula annotation. Using an evaporation‐based gas standard system, the signal response of the different solutions was probed.ResultsPrincipal component analysis revealed a substantial difference in features detected with AgNO3. With silver, more sulfur‐containing features and more unsaturated hydrocarbon compounds were detected. Furthermore, more primary amines were potentially ionized, as indicated by van Krewelen diagrams. In total, twice as many features were unique to AgNO3 than for other electrospray dopants. Using gas standards at known concentrations, the high sensitivity of FA as a dopant was demonstrated but also indicated alternate sensitivities of the other electrospray solutions.ConclusionsThis work demonstrated the potential of AgNO3 as a complementary dopant for further biomarker discovery in SESI‐based breath analysis.

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Effects of CPAP therapy withdrawal on exhaled breath pattern in obstructive sleep apnoea

Abstract: NCT02050425 (registered at ClinicalTrials.gov).

Cited by 56 publications

References 45 publications

Real-Time Quantification of Amino Acids in the Exhalome by Secondary Electrospray Ionization–Mass Spectrometry: A Proof-of-Principle Study

Real-Time Quantification of Amino Acids in the Exhalome by Secondary Electrospray Ionization–Mass Spectrometry: A Proof-of-Principle Study

Direct mass spectrometry analysis of exhaled human breath in real‐time

Alternative electrolyte solutions for untargeted breath metabolomics using secondary‐electrospray ionization high‐resolution mass spectrometry

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