Effects of coumaphos and imidacloprid on honey bee (Hymenoptera: Apidae) lifespan and antioxidant gene regulations in laboratory experiments

Gregorc, Aleš; Alburaki, Mohamed; Rinderer, Nicholas; Sampson, Blair J.; Knight, Patricia R.; Karim, Shahid; Adamczyk, John J.

doi:10.1038/s41598-018-33348-4

Cited by 75 publications

(69 citation statements)

References 42 publications

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“…Lack of experimental diet consumption does not appear to explain the results of our HPG investigation. Although mean pollen paste consumption (8.6 mg bee –1 per 3 days, SD = 7.69) was lower in our study compared to some in vitro studies, it was within the same range as Hatjina et al ., who found negative effects of pesticide exposure on HPG development . Similar to Hatjina et al ., mean pollen paste consumption at the start of our experiment, coinciding with HPG development, was significantly ( P < 0.001) elevated, up to 3‐fold greater than overall mean consumption.…”

Section: Discussionsupporting

confidence: 66%

Effects of chronic dietary thiamethoxam and prothioconazole exposure onApis melliferaworker adults and brood

Wood

Mattos

Kozii

et al. 2019

Pest Management Science

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BACKGROUND Chronic exposure of honey bees (Apis mellifera Linnaeus) to the neonicotinoid thiamethoxam and the fungicide prothioconazole is common during foraging in agricultural landscapes. We evaluated the survival and hypopharyngeal gland development of adult worker honey bees, and the survival of the worker brood when chronically exposed to thiamethoxam or thiamethoxam and prothioconazole in combination. RESULTS We found that 30 days of exposure to 40 μg kg–1 of thiamethoxam significantly (P < 0.001) increased the frequency of death in worker adults by four times relative to solvent control. The worker brood required 23 times higher doses of thiamethoxam (1 mg L–1 or 909 μg kg–1) before a significant (P = 0.04), 3.9 times increase in frequency of death was observed relative to solvent control. No additive effects of simultaneous exposure of worker adults or brood to thiamethoxam and prothioconazole were observed. At day 8 and day 12, the hypopharyngeal gland acinar diameter was not significantly different (P > 0.05) between controls and adult workers exposed to thiamethoxam and/or prothioconazole. CONCLUSION These results indicate that chronic exposure to field‐realistic doses of thiamethoxam and/or prothioconazole are unlikely to affect the survival of adult workers and brood. © 2019 Society of Chemical Industry

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Section: Discussionsupporting

confidence: 66%

Effects of chronic dietary thiamethoxam and prothioconazole exposure onApis melliferaworker adults and brood

Wood

Mattos

Kozii

et al. 2019

Pest Management Science

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“…Our previous study reported a reduction in the development of mandibular and hypopharyngeal glands in nurse honeybees exposed to pyraclostrobin and fipronil individually and in combination 15 . Other previous studies reported different responses of antioxidant system of honeybees exposed to pesticides or natural compounds, and these responses can change based on the age of the bee, time of exposure, dosage, and pesticide group 46,47,54,60,61 .…”

Section: Downregulation Of Antioxidant Enzymes and Increase In Suscepmentioning

confidence: 93%

Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides

Zaluski

Bittarello

Vieira

et al. 2020

Sci Rep

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Understanding the effect of pesticides on the survival of honeybee colonies is important because these pollinators are reportedly declining globally. In the present study, we examined the changes in the head proteome of nurse honeybees exposed to individual and combined pesticides (the fungicide pyraclostrobin and the insecticide fipronil) at field-relevant doses (850 and 2.5 ppb, respectively). The head proteomes of bees exposed to pesticides were compared with those of bees that were not exposed, and proteins with differences in expression were identified by mass spectrometry. The exposure of nurse bees to pesticides reduced the expression of four of the major royal jelly proteins (MRJP1, MRJP2, MRJP4, and MRJP5) and also several proteins associated with carbohydrate metabolism and energy synthesis, the antioxidant system, detoxification, biosynthesis, amino acid metabolism, transcription and translation, protein folding and binding, olfaction, and learning and memory. Overall, when pyraclostrobin and fipronil were combined, the changes in protein expression were exacerbated. Our results demonstrate that vital proteins and metabolic processes are impaired in nurse honeybees exposed to pesticides in doses close to those experienced by these insects in the field, increasing their susceptibility to stressors and affecting the nutrition and maintenance of both managed and natural colonies.Pollination is an indispensable service provided mainly by wild insects and farmed honeybees (Apis mellifera, Linnaeus, 1758) that supports biodiversity, agriculture, and food security 1,2 . There is growing concern over the reported global reduction in these insects, which compromises this ecological service 2-8 .The maintenance of honeybee colonies is dependent upon specialised tasks performed by individuals of different roles; thus, stress-related dysfunction of specialised workers can affect entire colonies 9,10 . Pesticides, phytochemicals, pathogens, and parasites are among the main stressors for honeybees, and exposure can lead to dysfunctions that change the behaviour, anatomy, and/or physiology of these insects 10 . Previous reviews have indicated that pesticide exposure negatively affects the maintenance of wild and commercial honeybee populations 5,11,12 . Exposure of bees to field-relevant doses of pesticides that are frequently found in plants visited by these insects compromise essential functions, such as cognition, foraging, navigation, homing, and memory [13][14][15] . Exposure also causes physiologic changes in individual bees that compromises colony maintenance 9,15-18 .Nutrition is a key factor in colony maintenance 19 and caste differentiation of honeybees, and is essential for the full development and activity of mandibular and hypopharyngeal (or brood-food) glands in the head of 6-day-old worker bees 20-22 . These glands are responsible for the production of royal jelly (RJ), a secretion used to nourish all bee larvae until 3 days old, after which only the queen larvae are fed with RJ throughout larval and a...

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“…Systemic infections in honey bees could be exacerbated by acaricide, neonicotinoid, or fungicide exposure, which reduces intestinal stem cell proliferation (Forkpah et al, 2014) and increases midgut apoptosis (Gregorc et al, 2018;Carneiro et al, 2019), potentially weakening the gut barrier. Hemocytes also function as phagocytic cells in the honey bee hemolymph; however exposure to neonicotinoids reduces hemocytes phagocytic activity (Walderdorff et al, 2018) and hemolymph antimicrobial activity (Brandt et al, 2016).…”

Section: Pesticides Disrupt Honey Bee Immunitymentioning

confidence: 99%

Understanding the Effects of Sublethal Pesticide Exposure on Honey Bees: A Role for Probiotics as Mediators of Environmental Stress

et al. 2020

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Managed populations of the European honey bee (Apis mellifera) support the production of a global food supply. This important role in modern agriculture has rendered honey bees vulnerable to the noxious effects of anthropogenic stressors such as pesticides. Although the deleterious outcomes of lethal pesticide exposure on honey bee health and performance are apparent, the ominous role of sublethal pesticide exposure is an emerging concern as well. Here, we use a data harvesting approach to better understand the toxicological effects of pesticide exposure across the honey bee life cycle. Through compiling adult-and larval-specific median lethal dose (LD 50) values from 93 published data sources, LD 50 estimates for insecticides, herbicides, acaricides, and fungicides are highly variable across studies, especially for herbicides and fungicides, which are underrepresented in the meta-data set. Alongside major discrepancies in these reported values, further examination of the compiled data suggested that LD 50 may not be an ideal metric for honey bee risk assessment. We also discuss how sublethal effects of pesticide exposure, which are not typically measured in LD 50 studies, can diminish honey bee reproduction, immunity, cognition, and overall physiological functioning, leading to suboptimal honey bee performance and population reduction. In consideration of actionable solutions to mitigate the effects of sublethal pesticide exposure, we have identified the potential for probiotic supplementation as a promising strategy that can be easily incorporated alongside current agricultural infrastructure and apicultural management practices. Probiotic supplementation is regularly employed in apiculture but the potential for evidence-based targeted approaches has not yet been fully explored within a formal toxicological context. We discuss the benefits, practical considerations, and limitations for the use and delivery of probiotics to hives. Ultimately, by subverting the sublethal effects of pesticides we can help improve the long-term survival of these critical pollinators.

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Effects of coumaphos and imidacloprid on honey bee (Hymenoptera: Apidae) lifespan and antioxidant gene regulations in laboratory experiments

Cited by 75 publications

References 42 publications

Effects of chronic dietary thiamethoxam and prothioconazole exposure onApis melliferaworker adults and brood

Effects of chronic dietary thiamethoxam and prothioconazole exposure onApis melliferaworker adults and brood

Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides

Understanding the Effects of Sublethal Pesticide Exposure on Honey Bees: A Role for Probiotics as Mediators of Environmental Stress

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