Effects of corticosteroids and their combinations with hyaluronanon on the biochemical properties of porcine cartilage explants

Siengdee, Puntita; Radeerom, Tiwaporn; Kuanoon, Similan; Euppayo, Thippaporn; Pradit, Waranee; Chomdej, Siriwadee; Ongchai, Siriwan; Nganvongpanit, Korakot

doi:10.1186/s12917-015-0611-6

Cited by 15 publications

(12 citation statements)

References 55 publications

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“…However, in this study, it was found that the combination treatment with CAR with HA (Comb24 and Comb48) did not decrease chondrotoxicity (and did not increase chondrocyte viability). Similar to our previous study involving OA chondrocyte cultures, co-treatment with HA did not decrease chondrotoxicity caused by CAR (Euppayo et al, 2015), triamcinolone acetonide (Euppayo et al, 2016), dexamethasone (Siengdee et al, 2015) and prednisolone (Siengdee et al, 2015). However, some studies have shown that HA could reduce cytotoxicity on chondrocyte viability after cells were exposed to bupivacaine (Onur, Sitron & Dang, 2013), indomethacin (Hashizume & Mihara, 2009), dexamethasone (Hashizume & Mihara, 2009) and celecoxib (Hashizume & Mihara, 2009).…”

Section: Discussionsupporting

confidence: 84%

“…However, HA could lessen the side effects of fluoroquinolones when treatment was given in conjunction with those other drugs (Siengdee et al, 2016). On the contrary, the therapeutic effects of HA could not mitigate the side effects of carprofen (Euppayo et al, 2015), corticosteroid (Siengdee et al, 2015) and triamcinolone acetonide (Euppayo et al, 2016) when subjects were treated with HA at the same time.…”

Section: Introductionmentioning

confidence: 99%

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Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture

Nganvongpanit

Euppayo

Siengdee

et al. 2020

PeerJ

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A major concern associated with the use of drugs is their adverse side effects. Specific examples of the drugs of concern include antibiotic agents and non-steroidal anti-inflammatory drugs. Despite the presence of a high degree of efficacy for specific conditions, these drugs may deteriorate the surrounding tissues that are exposed to them. Often, carprofen is used for joint inflammation; however, it may stimulate cartilage degradation which can then lead to osteoarthritis progression. In this study, hyaluronan was combined with carprofen treatment in three different applications (pre-treatment, co-treatment and post-treatment) on normal canine chondrocytes to determine whether Hyaluronan (HA) is capable of mitigating the degree of chondrotoxicity of carprofen. Our findings revealed that carprofen at IC20 (0.16 mg/mL) decreased viability and increased nitric oxide (NO) production. Importantly, carprofen induced the apoptosis of canine chondrocytes via the up-regulation of Bax, Casp3, Casp8, Casp9 and NOS2 as compared to the control group. Although the co-treatment of HA and carprofen appeared not to further alleviate the chondrotoxicity of carprofen due to the presence of a high number of apoptotic chondrocytes, post-treatment with HA (carprofen treatment for 24 h and then changed to HA for 24 h) resulted in a decrease in chondrocyte apoptosis by the down-regulation of Bax, Casp3, Casp8, Casp9, NOS2, along with NO production when compared with the treatment of carprofen for 48 h (P < 0.05). These results suggest that HA can be used as a therapeutic agent to mitigate the degree of chondrotoxicity of carprofen.

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Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture

Nganvongpanit

Euppayo

Siengdee

et al. 2020

PeerJ

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“…Thus, not only pain relief and suppression of inflammation should be addressed, but also cartilage repair. Recent reports have investigated the combined effects of HA and anti-inflammatory drugs (CSs or NSAIDs) [66,67] in order to develop more effective OA treatments. We evaluated the combined effects of HA with low concentrations of Dex and therapeutic concentrations of Dic or Ace on CSE-impaired chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

Primary Human Chondrocytes Affected by Cigarette Smoke—Therapeutic Challenges

Chen

Ehnert

Tendulkar

et al. 2020

IJMS

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Although several researchers have attested deleterious effects of smoking to the musculoskeletal system, the association between smoking and the onset of osteoarthritis (OA) remains unclear. Here, we investigate the effect of cigarette smoke extract (CSE) on primary human chondrocytes. The present study demonstrates that physiological concentrations of CSE (0.1%–10%) inhibit the viability, proliferation, and matrix formation of chondrocytes in a dose- and time-dependent manner. Significant amounts of free radicals were generated by 10% of CSE and led to cell death. A clinical dosage (4 mg/mL) of dexamethasone (Dex) showed toxic effects on chondrocytes, and the long-time treatment by lower doses (4–400 μg/mL) induced hypertrophic changes in the chondrocytes. To substitute Dex, diclofenac (Dic, 1 μg/mL) and acetaminophen (Ace, 10 μg/mL) were tested and did not worsen the metabolic activity of CSE-exposed chondrocytes. Hyaluronic acid (HA, 5 mg/mL) combined with Dic or Ace significantly inhibited the oxidative stress and enhanced the viability and matrix formation of CSE-exposed chondrocytes. This study shows for the first time that CSE mediates the disruption of cartilage through inducing cell death by increasing oxidative stress, and that this effect is fortified by Dex. The deleterious effects of CSE on chondrocytes could be reversed by treatment with HA combined with first-line analgesic/anti-inflammatory agents.

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“…In particular, there are concerns about reduced chondrocyte production and increasing apoptosis that eventually result in cartilage deterioration. However, no increased risks were reported with this combination, neither from clinical12 nor histological evidence 13…”

Section: Introductionmentioning

confidence: 88%

Corticosteroids injections versus corticosteroids with hyaluronic acid injections in rhizarthrosis: the randomised multicentre RHIZ’ART trial study protocol

Cormier

Goff²,

Denis

et al. 2019

BMJ Open

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IntroductionOsteoarthritis of the trapeziometacarpal joint affects approximately 10%–25% of women, especially those who are postmenopausal. It may result in thumb dysfunction. Among the treatments, intra-articular injections of corticosteroid (CS) and hyaluronic acid (HA) are both effective and recommended. However, clinical trials have shown that HA improves functional capacity, whereas CS only produces a decrease in pain. The synergy of these two drugs has not been evaluated. The primary goal of this study was to determine whether the association between HA and CS produce an additional decrease of more pain during thumb movement at three months postinjection, compared to the level of pain relief from CS alone.Methods and analysisRHIZ’ART is a prospective, multicentre, comparative, randomised, controlled, double-blind trial. Patients referred to the rheumatology department for thumb rhizarthrosis will receive an injection of betamethasone with HA or placebo (serum saline) based on central randomisation and stratification by centre. Injections will be given under ultrasound guidance. The primary outcome will compare the pain Visual Analogue Scale with motion at three months for both groups using a mixed model. The expected decrease in pain intensity in the CS group is 25% and 35% in the CS with HA group. In order to achieve a 80% power for detecting this difference with α set at 5%, 73 patients are needed in each group (146 total). The main secondary outcomes are the Cochin score (hand function) and grip strength. Follow-up visits are at 1, 3, 6 and 12 months.Ethics and disseminationThe study project has been approved by the appropriate ethics committee (CPP île de France III, 2017-002298-20). In agreement with current French regulations, a signed informed written consent will be obtained from each patient. Results of the main trial and of the secondary endpoints will be submitted for publication in a peer-reviewed journal.Trial registration number NCT03431584.

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Effects of corticosteroids and their combinations with hyaluronanon on the biochemical properties of porcine cartilage explants

Cited by 15 publications

References 55 publications

Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture

Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture

Primary Human Chondrocytes Affected by Cigarette Smoke—Therapeutic Challenges

Corticosteroids injections versus corticosteroids with hyaluronic acid injections in rhizarthrosis: the randomised multicentre RHIZ’ART trial study protocol

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