Effects of corticosteroid treatment and inflammation on the cellular content of blood and exudate in mice

Abstract

Inflammatory exudates have been produced in mice by intraperitoneal injection of thioglycollate broth 24 hr and again 3 hr before collecting of the exudate. The first injection of broth exerts a "priming" effect leading to an enhanced response to the second injection. By this procedure more than 30 x 106 leucocytes of which 78 per cent. were polymorphs were obtained from each mouse. Administration of paramethasone reduced the number of cells in the exudate when given at the same time as broth but not when given 3 hr before collection of the exudate. In contrast paramethasone was equally effective when given either 24 hr or 3 hr before harvest in suppressing the appearance of intravenously injected pontamine sky blue in the exudate. It was striking that treatment with paramethasone which had reduced the number of polymorphs in the exudate had actually increased the number in blood.

The requirement of an adherent cell substratum for the growth of developing plasmacytoma cells in vivo.

“…Thompson and VanFurth (7) demonstrated that HC prevented the migration of mononuclear cells into the inflamed peritoneal cavity, but did not reduce the existing population of peritoneal macrophages. Studies with HC and paramethasone indicate that these drugs have similar effects upon the PMNL component of inflammatory exudates (8,9), although the elevation of the numbers of these cells in the circulation was not prevented. These results suggest that if the pristane-induced APC are critical for the establishment of a conditioned environment, then HC treatment should abrogate the conditioning effect.…”

The effect of anti-inflammatory and anti-rheumatic drugs on cell accumulation onto nitrocellulose discs implanted subcutaneously into mice

“…The toxicity of these potent steroids was also less in the mouse than in the rat and so this may reflect a species difference in the way the steroids are handled. ATKINSON et al [20] have shown that dexamethasone is less active on thymus weight in mice than in rats, but paramethasone has been shown to be very effective at reducing peritoneal exudation in the mouse [21].…”