Effects of copper sulphate concentrations during in vitro maturation of bovine oocytes

Picco, Sebastián Julio; Rosa, Diana Esther; Anchordoquy, Juan Patricio; Seoane, Analía Isabel; Mattioli, Guillermo Alberto; Furnus, Cecilia Cristina

doi:10.1016/j.theriogenology.2011.08.009

Cited by 30 publications

(26 citation statements)

References 42 publications

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“…Previous studies demonstrated that both Zn and Cu also have similar values when plasma and FF concentrations were compared (Picco et al. , ). According to Kincaid's classification (), the values of Mn obtained in this study for plasma and FF correspond to adequate status in cattle.…”

Section: Discussionmentioning

confidence: 90%

“…The presence of micronutrients such us Zn or Cu during oocyte maturation not only increased GSH content and protect DNA integrity of cumulus cells, but also improved subsequent embryo development to the blastocyst stage (Picco et al. , ). In agreement with these previous studies, we have found a correlation between embryo production and Mn concentration in the IVM media.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Different Manganese Concentrations during in vitro Maturation of Bovine Oocytes on DNA Integrity of Cumulus Cells and Subsequent Embryo Development

Anchordoquy

Sirini

Mattioli

et al. 2013

Reprod Domestic Animals

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Manganese (Mn) is a trace element present in forages and cereals, and its concentration depends on soil status. Manganese deficiency in cattle, goats and ewes not only impairs oestrous cycle but reduces calf birth weight. The achievement of the first oestrus is delayed, and more attempts are necessary to obtain a successful conception. This study was conducted to investigate the effect of the availability of supplemental Mn during IVM on DNA damage of cumulus cells and total glutathione (GSH) content in oocytes and cumulus cells. The effect of supplementary Mn during IVM on subsequent embryo development was also studied. The results reported here indicate (i) DNA damage in cumulus cells decreased with 0, 2, 5 and 6 ng/ml Mn supplementation during IVM (p < 0.05). (ii) Intracellular GSH-GSSG content increased (p < 0.01) with different Mn concentrations in oocytes and cumulus cells. Also, cumulus cell number per cumulus oocyte-complexes (COC) did not differ either before or after IVM. (iii) Addition of Mn to maturation medium resulted in similar cleavage rates (p > 0.05) at 0, 2, 5 and 6 ng/ml Mn. However, subsequent embryo development to blastocyst stage was significantly higher (p < 0.01) in oocytes matured with 5 and 6 ng/ml Mn. (iv) There was also an increase (p < 0.05) in mean cell number per blastocyst obtained from oocytes matured with 5 and 6 ng/ml respect to zero Mn (IVM alone) and 2 ng/ml Mn. This study provides evidence that optimal embryo development to the blastocyst stage was partially dependent on the presence of Mn during IVM. Moreover, the availability of Mn during oocyte maturation ensures 'normal' intracellular GSH content in COCs and protects DNA integrity of cumulus cells.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Effect of Different Manganese Concentrations during in vitro Maturation of Bovine Oocytes on DNA Integrity of Cumulus Cells and Subsequent Embryo Development

Anchordoquy

Sirini

Mattioli

et al. 2013

Reprod Domestic Animals

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“…Among these trace elements, zinc, copper, and manganese play an essential role in reproduction. It has been demonstrated that low concentrations of zinc and copper during in vitro maturation (IVM) induce apoptosis and affect the DNA integrity of cumulus cells, which negatively influence the developmental competence of bovine oocytes (Anchordoquy et al, 2011;Picco et al, 2010Picco et al, , 2012. However, adequate zinc (1.5 g/mL) and manganese (6 ng/mL) concentrations during IVM increased the intracellular glutathione concentrations and protect the bovine COCs from apoptosis (Anchordoquy et al, 2014;Picco et al, 2010).…”

Section: Maternal Nutritionmentioning

confidence: 99%

Maternal control of oocyte quality in cattle “a review”

Moussa

Shen²,

Zhang

et al. 2015

Animal Reproduction Science

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“…Manganese concentration was measured by atomic absorption spectrophotometer (GBC 902) with graphite furnace through an internal quality control (Picco et al, 2012) and classified according to Kincaid (1999). The plasma was separated and the ovaries from these heifers were collected and handled independently to give 40 values for Mn in each follicle class.…”

Section: Reagents and Mediamentioning

confidence: 99%

“…High rates of apoptotic bovine follicular cells affect subsequent oocyte development (Zadák et al, 2009). Similarly, deficiency of copper concentrations during in vitro maturation of bovine oocytes affects DNA integrity of cumulus cells, and the competence (cytoplasmic maturation) of these oocytes to develop until the blastocyst stage (Picco et al, 2012). Adequate zinc concentrations added to IVM medium also improve cleavage rates after IVF of oocytes matured in vitro compared with those matured in zinc-deficient medium .…”

Section: Introductionmentioning

confidence: 99%

Influence of manganese on apoptosis and glutathione content of cumulus cells during in vitro maturation in bovine oocytes

Anchordoquy

Picco

et al. 2013

Cell Biology International

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We have investigated the effect of different Mn concentrations on (1) DNA integrity of cumulus cells by olive tail moment (OTM); (2) cumulus cells apoptosis by Annexin V staining assay; (3) intracellular total glutathione (GSH-GSSG) content; and (4) oocyte nuclear maturation and embryo cleavage after in vitro fertilisation (IVF). For this purpose, 0 (control), 2 (Mn1), 5 (Mn2) and 6 ng/mL (Mn3) Mn concentrations were added to IVM medium. Comet assay analysed by OTM was significantly higher in cumulus cells arising from COCs matured without Mn (control, P < 0.01) respect to cumulus cells obtained from COCs matured with Mn (control: 5.18 ± 2.3; Mn1: 2.93 ± 2.2; Mn2: 2.63 ± 2.4; Mn3: 2.92 ± 2.4). The frequency of apoptotic cells was higher in the control group (control: 6.63 ± 0.59; Mn1: 5.05 ± 0.5; Mn2: 4.61 ± 0.49; Mn3: 3.33 ± 0.42). Intracellular concentration of GSH-GSSG increased in oocytes and cumulus cells matured in the presence of Mn (P < 0.01). There were no differences in percentages of nuclear maturation when Mn was added to IVM medium at any concentration, but at 6 ng/mL Mn a higher cleavage rate was observed respect to the control group (P < 0.05). In conclusion, deficiency in Mn concentration during in vitro maturation increased the damage in the DNA molecule and the frequency of apoptotic cumulus cells. However, the addition of an adequate Mn concentration (6 ng/mL Mn) to IVM medium improved the health of cumulus-oocyte complexes and produced more cleaved embryos 48 h after IVF.

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Effects of copper sulphate concentrations during in vitro maturation of bovine oocytes

Cited by 30 publications

References 42 publications

Effect of Different Manganese Concentrations during in vitro Maturation of Bovine Oocytes on DNA Integrity of Cumulus Cells and Subsequent Embryo Development

Effect of Different Manganese Concentrations during in vitro Maturation of Bovine Oocytes on DNA Integrity of Cumulus Cells and Subsequent Embryo Development

Maternal control of oocyte quality in cattle “a review”

Influence of manganese on apoptosis and glutathione content of cumulus cells during in vitro maturation in bovine oocytes

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