Effects of copper pollution on the ultrastructure of Lessonia spp.

Leonardi, Patricia I.; Vásquez, Julio A.

doi:10.1007/978-94-011-4449-0_44

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…The range of Cd in the eight species of macroalgae from the reference sites, including L. nigrescens, were higher than those reported for algae in non-upwelling areas (25-28) but were within the range reported for species within upwelling regions (12). On the other hand, the levels of Cu found in the study species were in agreement with values previously reported in macroalgae from different parts of the world (26,29,30). However, the high concentration of this metal measured in algae native to Caleta Palito, and in L. nigrescens transplanted for 2 months to this site, agree only with what has been reported in marine algae from other contaminated coastal systems (29,31,32).…”

Section: Discussionsupporting

confidence: 89%

Cadmium−Copper Antagonism in Seaweeds Inhabiting Coastal Areas Affected by Copper Mine Waste Disposals

Andrade

Medina

Moffett

et al. 2006

Environ. Sci. Technol.

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Cadmium and copper accumulation by macroalgae was studied in a coastal area exposed to upwelling events and high levels of Cu, the latter resulting from mine disposals. Eight species were studied, and all had very high concentrations of Cd outside of the Cu-contaminated area. Cu in algal tissues was much higher in contaminated than in reference sites. High Cu appeared to suppress Cd bioaccumulation; Cd in algal tissues was much lower in the Cu-contaminated area than in the reference sites. Transplant experiments with Lessonia nigrescens revealed a depuration of Cd in individuals transplanted to areas with high Cu. However, Cd depuration occurs more slowly than Cu uptake. These differences suggest that while Cd and Cu are linked mechanistically, itis nota simple substitution. Overall, the work confirms that macroalgae are useful indicators of metal contamination and may be used as in situ biomonitors for labile forms of metals, like free Cu2+. However, antagonistic relationships between metals must be clearly understood in order to properly interpret their concentrations in macroalgae.

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Section: Discussionsupporting

confidence: 89%

Cadmium−Copper Antagonism in Seaweeds Inhabiting Coastal Areas Affected by Copper Mine Waste Disposals

Andrade

Medina

Moffett

et al. 2006

Environ. Sci. Technol.

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“…The accumulation in S. lomentaria was not completely unexpected, as similar results have been reported for U. compressa, the only species that coexists in large densities with S. lomentaria at the copper-enriched site (Ratkevicius et al 2003) and for a variety of algae inhabiting metal-enriched coastal environments around the world (Foster 1976, Strömgren 1980, Luoma et al 1982, Ho 1984, 1990, Forsberg et al 1988, Say et al 1990, Mardsen and DeWreede 2000. The high levels of copper in the tissues of S. lomentaria naturally growing at the impacted site was not reflected in changes at the organellar level, as reported for other seaweeds such as Lessonia nigrescens (Leonardi and Vásquez 1999) and Enteromorpha flexuosa (Andrade et al 2004). This apparent absence of effects on the structure of the cells may well be the result of the attenuation of copper toxicity due to the scavenging activity of the antioxidant system of S. lomentaria, as discussed below.…”

Section: Discussionsupporting

confidence: 84%

ANTIOXIDANT RESPONSES IN SCYTOSIPHON LOMENTARIA (PHAEOPHYCEAE) INHABITING COPPER‐ENRICHED COASTAL ENVIRONMENTS¹

Contreras¹,

Moenne²,

Correa³

2005

Journal of Phycology

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Scytosiphon lomentaria (Lingb.) Link. (Phaeophyceae) is one of the two dominant seaweeds in a coastal area of northern Chile affected by copper mine wastes, where the concentration of copper in water and algal tissues remains higher than in nonimpacted sites. Copper-loaded plants develop oxidative stress, as demonstrated by the increased levels of reactive oxygen species and lipoperoxides. This stress was associated with 1) an enhanced activity of the antioxidant enzymes catalase, glutathione peroxidase, ascorbate peroxidase, monodehydroascorbate reductase, and dehydroascorbate reductase and 2) an inhibition of the glutathione reductase activity. Furthermore, stressed plants showed a decrease in glutathione and phenolic compounds levels and an increase in total ascorbate. Reciprocal transplants revealed that plants rapidly adjusted their antioxidant system in response to the conditions of the receiving site. In individuals transplanted from the copper-enriched environment to the control site, normal levels of lipoperoxides and antioxidant compounds were restored in 48 h and antioxidant enzymes recovered their basal activities in 96 h. Individuals transplanted from the control site to the copper-enriched area adjusted their antioxidant compounds and antioxidant enzymes within 48 h and 96 h, respectively, and reached the functional status of the local plants. We conclude that S. lomentaria inhabiting the copperenriched area buffered oxidative stress by a simultaneous involvement of antioxidant enzymes and water-soluble antioxidant compounds. These antioxidant responses were rapid and reversible, suggesting that copper resistance in S. lomentaria is a constitutive trait and that copper enrichment of the area did not result in a locally adapted coppertolerant ecotype.

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“…2004). Intracellular copper accumulation was also revealed by accumulation of electron dense granules in various algal vacuoles from copper mine areas (Correa et al 1999;Leonardi and Vasquez 1999), where copper concentrations reach up to 6 mg L −1 (Castilla 1996) (similar to that in Cu3).…”

Section: Discussionmentioning

confidence: 84%

A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)

2014

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The pseudofilamentous red alga Chroodactylon ornatum was grown in f/2 culture medium with the addition of 10, 34, and 100 μM (nominal concentration) cupric sulfate. The bioassays were terminated at two selected end points (days 4 and 12). Growth inhibition, changes in pigment composition, and oxidative stress indicators such as phenolic compounds and lipid peroxidation (dosed as thiobarbituric reactive substances) were observed in cultures with 34 and 100-μM cupric sulfate. Quinacrine (Atebrin) and chlorotetracycline fluorochromes showed abundant vacuoles of acidic content, related with mucilage secretion. Structural analyses by methylation, desulfation-methylation, alkaline treatment, and NMR spectroscopy revealed that the mucilaginous sheath of C. ornatum contains a sulfated galactan with a backbone of alternating 3-linked β-D-galactopyranose and 4-linked α-L-galactopyranose moieties, i.e., an agaran. The absence of 3,6-anhydrogalactose and of its precursor unit (α-galactose 6-sulfate) were confirmed. The highly sulfated polysaccharide contained these ester groups on the C-2 and C-4 hydroxyl groups of the 3-linked unit and on C-3 of the 4-linked units. The large proportion of sulfate esterification in this polysaccharide can be related to the extracellular biosorption of copper divalent cation (2.5±0.4 mg per gram of dry weight) and to copper tolerance in bioassays.

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Effects of copper pollution on the ultrastructure of Lessonia spp.

Cited by 5 publications

References 23 publications

Cadmium−Copper Antagonism in Seaweeds Inhabiting Coastal Areas Affected by Copper Mine Waste Disposals

Cadmium−Copper Antagonism in Seaweeds Inhabiting Coastal Areas Affected by Copper Mine Waste Disposals

ANTIOXIDANT RESPONSES IN SCYTOSIPHON LOMENTARIA (PHAEOPHYCEAE) INHABITING COPPER‐ENRICHED COASTAL ENVIRONMENTS¹

A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)

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Effects of copper pollution on the ultrastructure of Lessonia spp.

Cited by 5 publications

References 23 publications

Cadmium−Copper Antagonism in Seaweeds Inhabiting Coastal Areas Affected by Copper Mine Waste Disposals

Cadmium−Copper Antagonism in Seaweeds Inhabiting Coastal Areas Affected by Copper Mine Waste Disposals

ANTIOXIDANT RESPONSES IN SCYTOSIPHON LOMENTARIA (PHAEOPHYCEAE) INHABITING COPPER‐ENRICHED COASTAL ENVIRONMENTS1

A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)

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ANTIOXIDANT RESPONSES IN SCYTOSIPHON LOMENTARIA (PHAEOPHYCEAE) INHABITING COPPER‐ENRICHED COASTAL ENVIRONMENTS¹