Effects of cooling rate on post-thaw motility and fertility of Japanese pearl oyster Pinctada fucata martensii spermatozoa

Arita, Kahori; Isowa, Kiyoshi; Ishikawa, Takashi; Aoki, Hideo; Ohta, Hiromi

doi:10.1007/s12562-012-0487-3

Cited by 4 publications

(1 citation statement)

References 12 publications

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“…In this study, the height of cm from the liquid nitrogen surface resulted in the highest post-thaw motility and PMI of all the heights evaluated, which differs from the optimal heights reported for other marine invertebrates. For example, the hight of cm was found to be the optimal in the black-lip pearl oyster [22], 5.2 cm in the green-lip and black-lip abalone [27,28] and 9-12.5 cm in the Japanese pearl oyster species [7,23]. Athough the optimal height differs among speceis, the results in published literature including this study suggest a narrow range of optimal height among marine invertebrates.…”

Section: Accepted Manuscriptmentioning

confidence: 50%

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Hassan

Qin

2017

Cryobiology

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Low survival of cryopreserved sperm impedes the application of cryopreservation technique in spermcasting oyster species. This study developed a simple method of liquid nitrogen vapor freezing to improve post-thaw sperm survival in the spermcasting oyster Ostrea angasi. The results indicate that the permeable cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG) and propylene glycol (PG) were non-toxic to sperm up to 20% concentration and 90 min exposure whereas methanol at 10% or higher was toxic to sperm for any exposure over 30 min. Among the treatments with permeable cryoprotectants, 15% EG produced the highest post-thaw sperm motility. Sperm motility was further improved by the addition of non-permeable cryoprotectants (trehalose and glucose), with 15% EG + 0.2 M trehalose resulting in the highest post-thaw sperm motility among all the combinations evaluated. The durations of 20, 30 and 60 min equilibrations produced a higher post-thaw sperm motility and plasma membrane integrity (PMI) than 10 min. Higher post-thaw motility and PMI were achieved by freezing sperm at the 8 cm height from the liquid nitrogen surface than at the 2, 4, 6, 10 or 12 cm height. Holding sperm for 10 min in liquid nitrogen vapor produced higher post-thaw motility and PMI than for 2, 5 or 20 min. The cryopreservation protocol developed in this study improved both post-thaw motility and PMI of O. angasi sperm at least 15% higher than those cryopreserved using programmable freezing method. Liquid nitrogen vapor freezing might have greater applicability in improving post-thaw sperm quality of spermcasting oyster species.

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Section: Accepted Manuscriptmentioning

confidence: 50%

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Hassan

Qin

2017

Cryobiology

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Development of a novel non-programmable cryopreservation method capable of accurate cooling rate manipulation

et al. 2018

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The effects of cryoprotectants on sperm motility of the Chinese pearl oyster, Pinctada fucata martensii

Zheng

Huang

et al. 2018

Cryobiology

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Cryopreservation has been widely employed to preserve genetic material of aquatic animals. Although of common use in bivalves, resulting effects due to the toxicity of the cryoprotectants dimethyl sulfoxide (DMSO), propanediol (PG), methanol (MET) and ethylene glycol (EG), upon sperm motility in the Chinese pearl oyster, Pinctada fucata martensii, has remained undocumented. This study endeavors to identify the least toxic among the effective cryoprotectant agents by observing and comparing their toxic effects on sperm motility under varying concentrations and duration of exposure. Sperm samples were exposed during controlled experiments, for 1, 3, 6, 9, 12 and 15 min durations, to each of the listed cryoprotectants at 5, 10, 15, and 20% (volume:volume) concentrations. Sperm motility was observed to diminish when exposed to all cryoprotectant solutions, and observations demonstrated that toxicity increased relative to both concentration and equilibration time. After 6 min of exposure to the cryoprotectants, sperm motility was seen to have diminished significantly in DMSO at just 5% concentration, and in MET, PG and EG at 10% concentrations, respectively (the values of the lowest observed effect concentrations). The relationship between the quantity of immotile sperm and the cryoprotectant concentration was described using the logarithmic regression equation. MET exhibited the lowest effective concentration required to inhibit sperm motility by 50% (EC), followed by EG, PG and DMSO, in order. Therefore, MET proved most toxic under the test conditions for sperm of P. fucata martensii, whereas DMSO, PG and EG were observed as comparatively safer, suggesting that DMSO, PG and EG warrant further study in the application of cryopreservation of Chinese P. fucata martensii sperm.

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Effects of cooling rate on post-thaw motility and fertility of Japanese pearl oyster Pinctada fucata martensii spermatozoa

Cited by 4 publications

References 12 publications

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Development of a novel non-programmable cryopreservation method capable of accurate cooling rate manipulation

The effects of cryoprotectants on sperm motility of the Chinese pearl oyster, Pinctada fucata martensii

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