Effects of Conversion of the Zinc-Binding Motif Sequence of Thermolysin, HEXXH, to That of Dipeptidyl Peptidase III, HEXXXH, on the Activity and Stability of Thermolysin

The extracellular contractile injection system is enriched in environmental microbes and associates with numerous toxins

“…DUF4157 is found in 13 phyla and has high eCIS specificity (odd ratio = 2614, q value = 0). It contains the Zn-binding motif HExxH, which characterizes many metalloproteases 34 – 37 . This domain, previously termed PVC-metallopeptidase, was identified as a marker of eCIS based on limited genome analyses 38 , 39 but its role has never been studied in a broad and functional context.…”

The extracellular contractile injection system is enriched in environmental microbes and associates with numerous toxins

“…DUF4157 is found in 13 phyla and has high eCIS specificity (odd ra o = 2614, q value = 0). It contains the Zn-binding mo f HExxH which characterizes many metalloproteases [32][33][34][35] . This domain, previously termed PVC-metallopep dase, was iden fied as a marker of eCIS based on limited genome analyses 36,37 but its role has never been studied in a broad and func onal context.…”

The specific applications of the TSR-based method in identifying Zn2+ binding sites of proteases and ACE/ACE2

“…Thermolysin [EC 3.4.24.27], originally identified in the culture broth of Bacillus thermoproteolyticus, is a thermostable zinc metalloproteinase [7] . Thermolysin has the consensus zinc-binding motif sequence, HExxH [8] . Two histidine residues of HExxH and one glutamate residue outside HExxH chelate the active-site Zn 2+ [8] .…”

“…Thermolysin has the consensus zinc-binding motif sequence, HExxH [8] . Two histidine residues of HExxH and one glutamate residue outside HExxH chelate the active-site Zn 2+ [8] . The glutamate of HExxH is important to catalytic activity [8] .…”