Effects of conventional CPB and mini-CPB on neutrophils CD162, CD166 and CD195 expression

Holmannová, Drahomíra; Koláčková, Martina; Mandak, Jiri; Kuneš, P; Holubcová, Zdeňka; Holubec, Tomáš; Krejsek, Jan

doi:10.1177/0267659116669586

Cited by 14 publications

(18 citation statements)

References 50 publications

(50 reference statements)

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“…Exclusion criteria were pregnancy, emergency operations and patients aged less than 18 years. Out of these 107 patients, 3 patients received "off-pump" cardiac surgery; these three patients were excluded from the analysis due a potentially different activation of immune cells that had not been exposed to extracorporeal circulation 16,17 . Patient data, clinical information and blood samples were collected prospectively immediately before (pre) surgery, after (post) surgery as well as 1 day and 4 days after cardiac surgery.…”

Section: Methodsmentioning

confidence: 99%

Elevated circulating CD14++CD16+ intermediate monocytes are independently associated with extracardiac complications after cardiac surgery

Mossanen

Jansen

Pracht

et al. 2020

Sci Rep

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Elective cardiac surgery has low procedural complications. However, about 40% of patients develop extracardiac complications including delirium and acute kidney injury. We hypothesized that inflammatory processes and immune cell activation might be associated with these complications. We therefore prospectively included 104 patients undergoing cardiac surgery in our study. We assessed peripheral blood leukocyte populations by flow cytometry and circulating cytokines before operation, after surgery and at days one and four post-operatively. Patients undergoing cardiac surgery showed significantly elevated leukocytes and neutrophils after surgery. On the contrary, monocytes decreased after surgery and significantly increased at days 1 and 4, particularly classical (Mon1,CD14++CD16−) and intermediate (Mon2,CD14++CD16+) monocytes. While peripheral leukocyte subsets were unaltered in patients with infectious (n = 15) or cardiac complications (n = 31), post-operative leukocytes (p = 0.0016), neutrophils (p = 0.0061) and Mon2 (p = 0.0007) were clearly raised in patients developing extracardiac complications (n = 35). Using multiple logistic regression analyses, patient's age, ICU days, number of blood transfusions and elevated post-surgery Mon2 independently predicted extracardiac complications. Our findings demonstrate that elevated Mon2 after cardiac surgery are associated with an increased risk for extracardiac complications. These findings might improve the risk estimation after cardiac operations and the role of Mon2 for inflammation in cardiac surgery.Despite advances in surgical and anesthetic techniques as well as enhanced postoperative care, cardiac surgery is still associated with a high risk of postoperative complications 1 . Besides typical cardiac (e.g., arrhythmia, myocardial infarction) and infectious complications (e.g., wound infection, pneumonia and sepsis), extracardiac non-infectious complications like acute kidney injury (AKI, 30%) and delirium (up to 26-52%) are frequent in patients after cardiac surgery 2,3 . It has been speculated that a large part of these complications could be explained by excessive inflammation caused by extracorporeal cardio-pulmonary bypass (CPB), hypothermia, myocardial ischemia and reperfusion and tissue damage due to the surgical procedure 4,5 . The influence of distinct immune cells and their subpopulations on the occurrence of post-operative complications is still ambiguous and needs further investigations. Particularly monocytes seems to play in important role within this context 6,7 .Circulating monocyte subsets represent a continuum of differentiation stages 8,9 . Using the surface markers CD14 (LPS receptor) and CD16 (FcγRIII), blood monocytes can be subdivided into three subpopulations, CD14++CD16− "classical" monocytes (Mon1), CD14++CD16+ "intermediate" (Mon2) and CD14-16+

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Section: Methodsmentioning

confidence: 99%

Elevated circulating CD14++CD16+ intermediate monocytes are independently associated with extracardiac complications after cardiac surgery

Mossanen

Jansen

Pracht

et al. 2020

Sci Rep

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“…Events were specifically gated on granulocytes. [Color figure can be viewed at wileyonlinelibrary.com] serious organ failures in the postoperative period, as reflected by a higher SOFA score on D0 (median SOFA: 6 [6][7] vs. 5 [4][5][6], P = 0.037) and on D1 (median SOFA: 2 [1][2][3][4][5] vs. 6 [4][5][6][7][8], P = 0.029). No other preoperative or intraoperative clinical and biological parameters were associated with early postoperative infectious complications ( Supplementary Table S4).…”

Section: Link Between Postoperative Leukocyte Subset and Infectious Cmentioning

confidence: 99%

Immature Granulocytes: A Risk Factor of Infection after Cardiac Surgery

Daix

Guérin

Tavernier

et al. 2018

Cytometry Part B Clinical

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Background Similarly, to sepsis, cardiac surgery with cardiopulmonary bypass (CPB) induces major changes in leukocyte subsets. Immature granulocytes (IGs) increase both in sepsis and after open‐heart surgery. Secondary infections are a major complication of cardiac surgery with CPB. We hypothesized that the assessment of leukocyte subsets with multicolor flow cytometry (FCM) could help the front‐line clinician to better identify patients at high risk of infectious complications in this clinical setting. Methods In this single‐center observational pilot study, we identified 26 leukocyte subsets using three combinations of antibodies (from 5 to 10 colors per combinations): one devoted to granulocytes, one to lymphocyte subpopulations and one for rare cells (plasma cells and dendritic cells). Blood samples were obtained preoperatively and immediately after open‐heart surgery under CPB in 59 patients without immuno‐depression, chronic or neoplastic inflammatory disease, and immunosuppressive treatment. Secondary infections during hospital stay were recorded. Results Patients exhibited postoperative NK and T‐cell lymphopenia, increased levels of IGs and monocytes with low levels of surface HLA DR. Twelve patients developed secondary infectious complications. Only immediate postoperative IG levels were significantly higher in these patient (6.6 [6; 7.39] G/L vs. 3.8 [2.67; 5.72] G/L, P = 0.01). Patients with immediate postoperative increase of IGs developed more frequently infectious complication (10/22 [46%] vs. 2/37 [5%]: P < 0.001). Conclusions This study suggests that postoperative increase of IGs is related to postoperative organ failure and promises to help in early identification of patients at risk of infectious complications after open‐heart surgery under CPB. © 2018 International Clinical Cytometry Society

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“…Alternatively, it fosters anti-inflammatory cascades by activating its cognate receptor, the CD200R (11). Low level of CD200 is associated with autoimmune disease progression, such as arthritis and neurodegenerative disorders (12). 4 Conversely, high CD200 expression promotes immune ignorance, which can enable cancer progression (12).…”

Section: Introductionmentioning

confidence: 99%

“…Low level of CD200 is associated with autoimmune disease progression, such as arthritis and neurodegenerative disorders (12). 4 Conversely, high CD200 expression promotes immune ignorance, which can enable cancer progression (12). As for the role of CD200 in lungs, the main focus has been on virus-induced inflammation, showing that CD200 limits both the amplitude and the duration of the inflammatory response (13).…”

Section: Introductionmentioning

confidence: 99%

Lung CD200 Receptor Activation Abrogates Airway Hyperresponsiveness in Experimental Asthma

Lauzon-Joset

Langlois

Lai

et al. 2015

Am J Respir Cell Mol Biol

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In allergic asthma, homeostatic pathways are dysregulated, which leads to an immune response toward normally innocuous antigens. The CD200-CD200 receptor pathway is a central regulator of inflammation, and CD200 expression was recently found to be down-regulated in circulating leukocytes of patients with asthma. Given the antiinflammatory properties of CD200, we investigated whether local delivery of recombinant CD200 (rCD200) could reinstate lung homeostasis in an experimental model of asthma. Brown Norway rats were sensitized with ovalbumin (OVA) and alum. rCD200 was intratracheally administered 24 hours before OVA challenge, and airway responsiveness to methacholine was measured 24 hours after the allergen challenge. Inflammation was also assessed by measuring cell recruitment and cytokine levels in bronchoalveolar lavages, as well as lung and draining lymph node accumulation of dendritic cells (DCs) and T cells. In sensitized rats, rCD200 abolished airway hyperresponsiveness, whereas the sham treatment had no effect. In addition, rCD200 strongly reduced OVA-induced lung accumulation of myeloid DCs, CD4(+) T cells, and T helper type 2 cells. This was associated with a strong reduction of OVA-induced IL-13 level and with an increase of IL-10 in supernatants of bronchoalveolar lavages. Lung eosinophilia and draining lymph node accumulation of myeloid DCs and T cells were not affected by rCD200. Overall, these data reveal that rCD200 can inhibit airway hyperresponsiveness in a model of asthma by a multistep mechanism associated with local alterations of the T cell response and the cytokine milieu.

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Effects of conventional CPB and mini-CPB on neutrophils CD162, CD166 and CD195 expression

Cited by 14 publications

References 50 publications

Elevated circulating CD14++CD16+ intermediate monocytes are independently associated with extracardiac complications after cardiac surgery

Elevated circulating CD14++CD16+ intermediate monocytes are independently associated with extracardiac complications after cardiac surgery

Immature Granulocytes: A Risk Factor of Infection after Cardiac Surgery

Lung CD200 Receptor Activation Abrogates Airway Hyperresponsiveness in Experimental Asthma

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