Effects of conjugated linoleic acid on the expression levels of miR-27 and miR-143 in pig adipose tissue

Qi, Renli; Chen, Y; Huang, Jinxiu; Yang, Feiyun

doi:10.4238/2015.june.26.7

Cited by 7 publications

(7 citation statements)

References 14 publications

(17 reference statements)

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“…Further understanding of the pathways through which CLA mediates its effect on monocytes and macrophages is critical in identifying regulators that drive atherosclerotic regression including microRNAs (miRs) which govern macrophage phenotype. The effects of CLA on miRs have been previously documented in adipose tissue (Parra et al., 2010; Qi et al., 2015), intestinal epithelial cells (Daimiel-Ruiz et al., 2015), and ovarian cancer cells (Shahzad et al., 2018).…”

Section: Conjugated Linoleic Acid and Atherosclerosismentioning

confidence: 99%

miR-155 in the Resolution of Atherosclerosis

2019

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Atherosclerosis is a chronic progressive inflammatory disease where advanced lesions can eventually completely obstruct blood flow resulting in clinical events, such as a myocardial infarction or stroke. Monocytes and macrophages are the dominant biologically active immune cells involved in atherosclerosis disease and play a pivotal role during initiation, progression, and regression of disease. Altering macrophage inflammation is critical to induce regression of atherosclerosis and microRNAs (miRs) have emerged as key regulators of the macrophage phenotype. MiRs are small noncoding RNAs that regulate gene expression. They are dysregulated during atherosclerosis development and are key regulators of macrophage function and polarization. MiRs are short nucleotide transcripts that are very stable in circulation and thus have potential as therapeutics and/or biomarkers in the context of atherosclerosis. Of relevance to this review is that inhibition of macrophage-specific miR-155 may be a viable therapeutic strategy to decrease inflammation associated with atherosclerosis. However, further studies on these miRs and advancements in miR therapeutic delivery are required for these therapeutics to advance to the clinical setting. Conjugated linoleic acid (CLA), a pro-resolving lipid mediator, is an agonist of the peroxisome proliferator-activated receptor (PPAR)-γ. The biological activities of CLA have been documented to have anti-atherogenic effects in experimental models of atherosclerosis, inducing regression and impacting on monocyte and macrophage cells. Our work and that of others on PPAR-γ agonists and polyunsaturated fatty acids have shown that these mediators regulate candidate miRNAs and promote pro-resolving atherosclerotic plaque microenvironments.

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Section: Conjugated Linoleic Acid and Atherosclerosismentioning

confidence: 99%

miR-155 in the Resolution of Atherosclerosis

2019

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“…For instance, four miR-NAs, miR-23a [36], miR-27a [37], miR-139-5p [38], and mmu-let-7d-5p [39], are negative regulators of 3T3-L1 adipocyte differentiations, which inhibit cell differentiation and decrease adipogenesis by repressing the gene expression of PPARγ. Consistently, miRNAs, such as miR-27 [40], miR-33b [41], and miR-143 [40], were also identified to repress the preadipocyte differentiation and development in the in vivo studies using porcine models. Interestingly, miRNAs could be positive regulators in adipocyte differentiation too.…”

Section: Post-transcriptional Regulation Of Pparγmentioning

confidence: 59%

Advances on PPARγ Research in the Emerging Era of Precision Medicine

Zhao

2018

CDT

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This article systematically reviews PPARγ's mechanisms of action and multilayer regulations. In addition, novel classes of therapeutics modulating PPARγ and new direction of research on genetic variants that affect PPARγ function and antidiabetic drug response are highlighted, which sheds light on PPARγ as a promising target for developing safer and precision medicine based therapeutic strategies.

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“…Taking into account the highly polymorphic nature of the porcine FABP4 gene 36 and a possible regulatory role of miRNAs in the FABP4 gene expression 37 , the 3′UTR of the FABP4 gene was amplified and sequenced using 10 animals with extreme values of FABP4 mRNA expression in BF. A polymorphism in the 3′UTR ( FABP4:g.6723 A > G ) was identified and genotyped in the 115 BC1_LD animals.…”

Section: Resultsmentioning

confidence: 99%

Expression analysis of candidate genes for fatty acid composition in adipose tissue and identification of regulatory regions

Revilla

Puig-Oliveras

Crespo-Piazuelo

et al. 2018

Sci Rep

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The aim of this work was to study the genetic basis of the backfat expression of lipid-related genes associated with meat quality traits in pigs. We performed a genome-wide association study with the backfat gene expression measured in 44 genes by qPCR and the PorcineSNP60 BeadChip genotypes in 115 Iberian x Landrace backcross animals. A total of 193 expression-associated SNPs located in 19 chromosomal regions were associated with expression levels of ACSM5, ELOVL6, FABP4, FADS2, and SLC27A4 genes. Three expression quantitative trail loci (eQTLs) corresponding to ACSM5, FABP4, and FADS2 were classified as cis-acting eQTLs, whereas the remaining 16 eQTLs have trans-regulatory effects. Remarkably, a SNP in the ACSM5 promoter region and a SNP in the 3′UTR region of FABP4 were the most associated polymorphisms with the ACSM5 and FABP4 expression levels, respectively. Moreover, relevant lipid-related genes mapped in the trans-eQTLs regions associated with the ACSM5, FABP4, FADS2, and SLC27A4 genes. Interestingly, a trans-eQTL hotspot on SSC13 regulating the gene expression of ELOVL6, ELOLV5, and SCD, three important genes implicated in the elongation and desaturation of fatty acids, was identified. These findings provide new data to further understand the functional regulatory mechanisms implicated in the variation of fatty acid composition in pigs.

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Effects of conjugated linoleic acid on the expression levels of miR-27 and miR-143 in pig adipose tissue

Cited by 7 publications

References 14 publications

miR-155 in the Resolution of Atherosclerosis

miR-155 in the Resolution of Atherosclerosis

Advances on PPARγ Research in the Emerging Era of Precision Medicine

Expression analysis of candidate genes for fatty acid composition in adipose tissue and identification of regulatory regions

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