Effects of condensed tannins from Leucaena on methane production, rumen fermentation and populations of methanogens and protozoa in vitro

Tan, Hui Yin; Sieo, Chin Chin; Abdullah, Norhafizah; Liang, Juan Boo; Huang, Xiaowu; Ho, Yin Wan

doi:10.1016/j.anifeedsci.2011.07.004

Cited by 187 publications

(161 citation statements)

References 52 publications

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“…Furthermore, results from screening studies Durmic et al, 2010) are often inconclusive and may be conflicting due to variation in dosage, chemical structure of the test substance or compound, diet, combination of treatments applied, adaptation of rumen microbes or the form in which an agent is introduced into the system (Cardozo et al, 2004. Substantial decreases of CH 4 production in vitro (Tan et al, 2011) have been reported, but in several cases these have been accompanied by adverse effects on feed degradation, with the implication that a similar effect may occur in vivo which would compromise diet digestibility and animal performance.…”

Section: Tablementioning

confidence: 99%

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Yáñez-Ruíz

Bannink

Dijkstra

et al. 2016

Animal Feed Science and Technology

122

115

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a b s t r a c tIn vitro fermentation techniques (IVFT) have been widely used to evaluate the nutritive value of feeds for ruminants and in the last decade to assess the effect of different nutritional strategies on methane (CH4) production. However, many technical factors may influence the results obtained. The present review has been prepared by the 'Global Network' FACCE-JPI international research consortium to provide a critical evaluation of the main factors that need to be considered when designing, conducting and interpreting IVFT experiments that investigate nutritional strategies to mitigate CH 4 emission from ruminants. Given the increasing and wide-scale use of IVFT, there is a need to critically review reports in the literature and establish what criteria are essential to the establishment and implementation of in vitro techniques. Key aspects considered include: i) donor animal species and number of animal used, ii) diet fed to donor animals, iii) collection and processing of rumen fluid as inoculum, iv) choice of substrate and incubation buffer, v) incubation procedures and CH 4 measurements, vi) headspace gas composition and vii) comparability of in vitro and in vivo measurements. Based on an evaluation of experimental evidence, a set of technical recommendations are presented to harmonize IVFT for feed evaluation, assessment of rumen function and CH 4 production.

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Section: Tablementioning

confidence: 99%

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Yáñez-Ruíz

Bannink

Dijkstra

et al. 2016

Animal Feed Science and Technology

122

115

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“…Recently, Rira et al (2015) found that lower CH4 production were induced with the supplementation of Acacia cyanophylla, a tanniferous plant showing high CT content (631 g/kg DM). Tan et al (2011) found that low levels of CT extracted from LL could manipulate rumen fermentation in CH4 production in terms of diverting H2 away from CH4 formation by decreasing methanogen and protozoa populations. Newbold et al (2005) suggested that the succinate propionate pathway to produce propionate by using propionate precursors (i.e) acrylate which can reduce CH4 with increase in acetate, propionate and TSCFA.…”

Section: Performed In Vivo Experiments By Supplementingmentioning

confidence: 99%

“…In addition, compounds present in LL can improve rumen function with increase in number of microbial growth especially cellulolytic and proteolytic bacteria (HOOVER;STOKES, 1991;TAN et al, 2011). There is very few in vivo studies RODRIGUEZ et al, 2015) using LL on both rumen function and CH4…”

Section: Introductionmentioning

confidence: 99%

“…Similiarly, LL has been shown anti-methanogenic properties in vitro and in vivo 2013). In addition, compounds present in LL can improve rumen function with increase in number of microbial growth especially cellulolytic and proteolytic bacteria (HOOVER;STOKES, 1991;TAN et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…LL leaves contain high level of protein, which can be able to replace soyabean meal in ruminants diets, and also contain tannins which could reduce CH4 production 2013;TAN et al, 2011). To overcome the possible restrictive effects of tannins, using polyethylene glycol (PEG) as a tannin binding agent forming tannin-PEG complexes have been used to determine the effects of tannin content in browse species on rumen metabolism TOLERA et al 1997).…”

Section: Introductionmentioning

confidence: 99%

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Mitigating the greenhouse gas balance of ruminant production by identifying plants with high tannin concentration and quantifying the methane emission in vivo

Dhanasekaran¹

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Dados Internacionais de Catalogação na Publicação (CIP) Seção Técnica de Biblioteca -CENA/USP Dhanasekaran Dinesh KumarMitigating the greenhouse gas balance of ruminant production by identifying plants with high tannin concentration and quantifying the methane emission in vivo.Mitigando o equilíbrio de gases do efeito estufa na produção de ruminantes pela identificação de plantas com concentração elevada de tanino e quantificação das emissões de metano in vivo / Dinesh Kumar Dhanasekaran; If you want to shine like a sun, first burns like a sun. Research is never the effort of a single individual and it is a team work and many have put their effort in several ways to bring this thesis to completion. and degraded neutral detergent fiber (DNDF), especially LL, which had most influence on these parameters compared to other tannin containing plants. LL had positive response on antimethanogenic effects; its nutrient degradability was higher than that of other tannin containing plants. The second study (Expt. 2) was set to evaluate the effect of different organic extracts from the whole plant methanolic extract (MHE) of LL on in vitro gas production and to characterize the chemical constituents by using gas chromatography coupled with mass spectroscopy (GC-MS). Major abundant compounds present at the relative percentages of MHE were found to be stigmasterol trimethyl ester (TMS), neophytadiene, palmitic acid TMS, α-Linolenic acid TMS and 2, 3, 5, 6-tetra methyl anisylbenzene. The effects of additions of different extracts in terms of nutrient degradability (TDOM and DNDF) were increased by all extracts. This study explained that the methanolic hexane extract and methanolic chloroform extract was effective against methanogenic activity. The objective of the third study (Expt. 3) was to study the effect of LL plant leaves on rumen fermentation, apparent nutrient digestibility, nitrogen balance and methane production in Santa Ines sheep. The animals were divided in three groups in which they were fed with (i) 88% Tifton 85-hay (Cynodon spp.) and 12% soyabean meal (Control group, n=4); (ii) 28% Tifton 85-hay (Cynodon spp.) and 72% LL plus 20 ml solution containing 10g/day/animal of PEG (With PEG group -WPEG, n=6); (iii) 28% Tifton 85-hay (Cynodon spp.) and 72% LL plus 20 ml of distilled water (without PEG group-WOPEG, n=6). Nutrient intake (dry matter, organic matter, acid detergent fiber, lignin and crude protein) were higher in WPEG and WOPEG compared to the control group, except neutral detergent fiber intake. Apparent digestibilities and nitrogen metabolism had non-significant effects between the treatments. However, CH4 emissions were significantly lower in WPEG and WOPEG than the control. Furthermore, expressions of microbial populations of methanogens in WPEG had lower tendency than that of WOPEG and control. The most salient findings of this study were that, 72% LL plant leaves using in small ruminants diets increased animal productivity, we can get more benefits in terms of replacing the source of protein in the...

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Soil greenhouse gas flux and nitrogen mineralization following manure application from tannin‐fed dairy cows

Romanko,

Gay,

Powell

et al. 2023

J of Env Quality

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Growing concerns about environmental impacts of dairy farms have driven producers to address greenhouse gas (GHG) emissions and nitrogen (N) losses from soil following land application of dairy manure. Tannin dietary additives have proved to be a successful intervention for mitigating GHG and ammonia (NH3) emissions at the barn scale. However, it is unknown how land application of dairy manure from cows fed tannin diets affects crop–soil nitrogen dynamics and soil GHG flux. To test this, cows were fed diets at three levels of tannins (0.0%, 0.4%, and 1.8% of dry matter intake) and their manure was field applied at two N rates (240 and 360 kg N ha−1). Soil NH4+‐N, NO3−‐N, corn silage yield, and soil GHG flux were then measured over a full growing season. Soils amended with tannin manure had lower initial NH4+‐N concentrations and lower total mineral N (NH4+‐N + NO3−‐N) concentrations 19 days after application, compared to soils amended with no tannin manures. Despite lower early season N availability in tannin‐fertilized plots, there were no differences in corn silage yield. No differences in soil GHG and NH3 emissions were observed between manure‐amended treatments. These results demonstrate that while tannin addition to dairy cow feed does not offer short‐term GHG or NH3 emissions reductions after field manure application, it can promote slower soil N mineralization that may reduce reactive N loss after initial application.

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Effects of condensed tannins from Leucaena on methane production, rumen fermentation and populations of methanogens and protozoa in vitro

Cited by 187 publications

References 52 publications

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Mitigating the greenhouse gas balance of ruminant production by identifying plants with high tannin concentration and quantifying the methane emission in vivo

Soil greenhouse gas flux and nitrogen mineralization following manure application from tannin‐fed dairy cows

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