Effects of concentration variation on the physical properties of alginate-based substrates and cell behavior in culture

Li, Liying; Chen, Yongzhi; Wang, Yiwei; Shi, Fuqian; Nie, Yi; Liu, Tianqing; Song, Kedong

doi:10.1016/j.ijbiomac.2019.01.123

Cited by 29 publications

(24 citation statements)

References 47 publications

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“…Results showed that the diameter of the microcapsules increased with increasing SA concentration, while the permeability decreased from 84.23 to 71.86%. This finding is consistent with the study of Li et al (2019). The maximum floating ability (70.83%) was achieved at a SA concentration of 3% (Table 1).…”

Section: Concentration Of Sasupporting

confidence: 92%

“…The permeability of the microcapsules decreased from 83.03 to 71.45% with an increase in CaCl 2 concentration. The study of Li et al (2019) also showed that the increase of Ca 2+ concentration reduced the permeability of alginate-based beads. The maximum floating ability (76.19%) was achieved when the CaCl 2 concentration was 3%.…”

Section: Concentration Of Caclmentioning

confidence: 92%

“…With further increase in Ca 2+ concentration, the floating ability decreased. It was reported that the gel strength of alginate increased exponentially within a concentration range of Ca 2+ (Draget et al, 1993), and the thickness of the external boundary of SA beads increased with the increase of CaCl 2 concentration (Li et al, 2019). When the concentration of CaCl 2 is higher than 3%, the binding sites When conducting the single factor effect experiments, except for the variables, the basic experimental conditions were 3% SA, CaCO 3 : SA of 3:4, 3% CaCl 2 , 3% citric acid, 2% EC, and crosslinking for 30 min).…”

Section: Concentration Of Caclmentioning

confidence: 99%

“…A low concentration of SA results in a flaccid microstructure for the microcapsules, thus the generated gas cannot be efficiently wrapped which resulted in a low floating ability. With an increase in SA concentration, the ionic crosslinking between Ca 2+ and SA resulted in a more compact structure of the alginate capsules (Li et al, 2019). However, microcapsules with a rigid microstructure could prevent H + from entering into the solution and reacting with CaCO 3 to make sufficient CO 2 gas, thus reducing the floating ability.…”

Section: Concentration Of Samentioning

confidence: 99%

“…Gel strength was dependent on the degree of interaction between SA and Ca 2+ ions. The surface microchannel's width of the alginate capsules was also affected by the degree of crosslinking (Li et al, 2019). A sufficient crosslinking time enables the internal gelation of the Ca 2+ and SA as well as the reaction of CaCO 3 and citric acid, thus enabling the generation of CO 2 and the wrapping of CO 2 gas.…”

Section: Crosslinking Timementioning

confidence: 99%

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Encapsulation and Algicidal Properties of Fermentation Products From Vibrio brasiliensis H115

Ouyang

Liu²,

Chen

et al. 2021

Front. Mar. Sci.

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Algicidal bacteria offer an eco-friendly and promising approach for controlling harmful algae blooms (HABs). In this study, repeated batch fermentation of immobilized algicidal bacterium Vibrio brasiliensis H115 was preformed to enhance the productivity of the algicidal compounds. The highest algicidal efficiency of the fermentation products against Akashiwo sanguinea (100%) was achieved when the fermentation time was decreased from 24 to 14 h. The cell-free fermentation broth was then spray-dried and floating microcapsules were prepared from the dried powder. The optimum preparation conditions for floating microcapsules were: sodium alginate (SA), 3%; CaCO3: SA (mass ratio), 3:4; CaCl2, 3%; citric acid, 4%; ethylcellulose, 2%; crosslinking time, 30 min. Under the optimal conditions, the floating microcapsules displayed efficient A. sanguinea cell lysis ability and the algicidal efficiency increased from 10.62% (4 h) to 100% (24 h). These results suggest that the floating microcapsules could potentially be practically used for controlling the outbreaks of A. sanguinea.

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Section: Concentration Of Sasupporting

confidence: 92%

Section: Concentration Of Caclmentioning

confidence: 92%

Section: Concentration Of Caclmentioning

confidence: 99%

Section: Concentration Of Samentioning

confidence: 99%

Section: Crosslinking Timementioning

confidence: 99%

See 3 more Smart Citations

Encapsulation and Algicidal Properties of Fermentation Products From Vibrio brasiliensis H115

Ouyang

Liu²,

Chen

et al. 2021

Front. Mar. Sci.

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Cytoprotection of Human Progenitor and Stem Cells through Encapsulation in Alginate Templated, Dual Crosslinked Silk and Silk–Gelatin Composite Hydrogel Microbeads

Hasturk

Smiley

Arnett

et al. 2022

Adv Healthcare Materials

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Susceptibility of mammalian cells against harsh processing conditions limit their use in cell transplantation and tissue engineering applications. Besides modulation of the cell microenvironment, encapsulation of mammalian cells within hydrogel microbeads attract attention for cytoprotection through physical isolation of the encapsulated cells. The hydrogel formulations used for cell microencapsulation are largely dominated by ionically crosslinked alginate (Alg), which suffer from low structural stability under physiological culture conditions and poor cell–matrix interactions. Here the fabrication of Alg templated silk and silk/gelatin composite hydrogel microspheres with permanent or on‐demand cleavable enzymatic crosslinks using simple and cost‐effective centrifugation‐based droplet processing are demonstrated. The composite microbeads display structural stability under ion exchange conditions with improved mechanical properties compared to ionically crosslinked Alg microspheres. Human mesenchymal stem and neural progenitor cells are successfully encapsulated in the composite beads and protected against environmental factors, including exposure to polycations, extracellular acidosis, apoptotic cytokines, ultraviolet (UV) irradiation, anoikis, immune recognition, and particularly mechanical stress. The microbeads preserve viability, growth, and differentiation of encapsulated stem and progenitor cells after extrusion in viscous polyethylene oxide solution through a 27‐gauge fine needle, suggesting potential applications in injection‐based delivery and three‐dimensional bioprinting of mammalian cells with higher success rates.

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Solvent types used for the preparation of hydrogels determine their mechanical properties and influence cell viability through gelatine and calcium ions release

et al. 2022

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Alginate-gelatin hydrogels are the most commonly used materials for 3D bioprinting.Their printability depends on their properties, and these derive from the way they are prepared and their very composition. Therefore, the aim of the study was to investigate the type of solvent (deionized water, phosphate buffer, and culture medium) and contents of gelatin in the composition of hydrogel (2% wt/vol alginate, 6% and 9% wt/vol of gelatin) on their biological, physicochemical, and mechanical properties, as well as printability and the ability of cells to proliferate in the printed structures. The results obtained revealed that all the manufactured hydrogel materials are biocompatible. The use of deionized water as a solvent results in the highest degree of cross-linking of hydrogels, thus obtaining a polymer with the highest rigidity. Moreover, an increase in gelatin content leads to an increase in the Young's modulus value, irrespectively of the solvent in which the hydrogels were prepared. Based on the chemical structure, it is more reasonable to use a culture medium for bioink preparation due to free NH and NH 2 groups being present, which are ligands for cell attachment and their proliferation. For the selected material (2A9GM), the printability and high viability of the cells after printing were confirmed. In this case, the concentration of the cross-linking agent influences gelatin amount release and calcium ions release, and these two processes determine the change in the viability of the cells encapsulated in the bioink.

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Effects of concentration variation on the physical properties of alginate-based substrates and cell behavior in culture

Cited by 29 publications

References 47 publications

Encapsulation and Algicidal Properties of Fermentation Products From Vibrio brasiliensis H115

Encapsulation and Algicidal Properties of Fermentation Products From Vibrio brasiliensis H115

Cytoprotection of Human Progenitor and Stem Cells through Encapsulation in Alginate Templated, Dual Crosslinked Silk and Silk–Gelatin Composite Hydrogel Microbeads

Solvent types used for the preparation of hydrogels determine their mechanical properties and influence cell viability through gelatine and calcium ions release

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