Effects of compressed unsaturated hydrocarbon gases on yeast growth

Abstract

The effect of compressed unsaturated hydrocarbon gases on the growth of the yeast Saccharomyces cerevisiae was investigated by microcalorimetry. The growth thermograms showed that unsaturated hydrocarbon gases inhibited yeast growth. As an approach to determining the comparative toxicity of unsaturated hydrocarbon gases, we determined the 50% inhibitory pressure (IP(50)) and the minimum inhibitory pressure (MIP). On the basis of the IP(50) and MIP values, the inhibitory potency of the gases increased in the order ethylene < propylene < 1-butene. Additionally, scanning electron microscopy showed that cells treated with unsaturated hydrocarbon gases were damaged, including invagination of the cell surface.

Effects of Gas Pressurization with Ethylene on the Ultrastructure of the YeastSaccharomyces cerevisiae

“…It was also found that pressurization with hydrocarbon gas induced deformity of the cell surface in small numbers of yeast cells. 2,3) We expect to find that gas pressurization with hydrocarbon impairs intracellular organelles in yeast. Little information is available on changes in intracellular organelles induced by gas pressurization, especially with ethylene.…”

“…It is often used as a model eukaryote for rapid and easy bioassay. [2][3][4][5][6] Saccharomyces cerevisiae IFO10149 was grown at 30 C in YPD medium (20 g/L glucose, 20 g/L peptone, and 10 g/L yeast extract) until it reached normal log-phase growth (O.D. at 660 nm, 0.9).…”