Effects of compound sodium nitrophenol on microspore embryogenesis and plantlet regeneration in flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee)

Fang, Siyu; Li, Jie; Zheng, Wenfeng; Li, Yong; Feng, Hui; Zhang, Yun

doi:10.1007/s00709-022-01771-0

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…Zeng et al [35] found that only 7 of the 11 genotypes could obtain microspore embryos in the study of stalks. Fang et al [36] applied 5 genotypes of flowering Chinese cabbage for IMC, and significant differences were sensed embryo induction rate among sundry genotypes. In this paper, three genotypes of cut flower ornamental kale cultivars were employed for IMC, and the embryo induction rates among the three genotypes were significantly different.…”

Section: Discussionmentioning

confidence: 99%

Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (Brassica oleracea var. acephala)

et al. 2022

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Cut flower ornamental kale (Brassica oleracea var. acephala) is a biennial cultivar, which completes a sexual reproductive generation in two years. Isolated microspore culture (IMC) can accelerate plant homozygosity instead of self-pollinations. However, the application of IMC in cut flower ornamental kale was rare since its low rate of embryogenesis. It is proved that histone acetylation might affect the gene expression in microspores and led to the transformation of microspores from pollen development pathway to embryogenesis. In this paper, microspores, derived from three varieties of cut flower ornamental kale, Crane Bicolor (CB), Crane Pink (CP) and Crane Feather Queen (CFQ), were treated with histone deacetylation inhibitor (HDACI) trichostatin A (TSA). Results revealed that the appropriate concentration of TSA was 10 nM for CB with obtaining 5.39 embryos per bud, while for CP and CFQ was 5 nM with acquiring 10.89 and 16.99 embryos per bud, respectively. TSA treatment also reduced the embryonic mortality, of which 10 nM TSA treatments CB was the optimal and the embryonic mortality decreased to 25.01%. The double haploid (DH) proportion of regenerated plants reached 37.3%. These results contribute to improving the technology for IMC in cut flower ornamental kale.

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Section: Discussionmentioning

confidence: 99%

Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (Brassica oleracea var. acephala)

et al. 2022

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Thidiazuron Promoted Microspore Embryogenesis and Plant Regeneration in Curly Kale (Brassica oleracea L. convar. acephala var. sabellica)

et al. 2023

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Curly kale (Brassica oleracea L. convar. acephala var. sabellica), the most common type of edible kale, characterized by providing rich nutrition and health care functions, is sought after and has been listed as top of the healthiest vegetables in recent trends, and has aroused the interest of breeders in cultivating new varieties. However, it usually takes more than six years to obtain a homozygous kale inbred line for commercial seed production through conventional breeding procedures due to its long growth and development period. The isolated microspore culture (IMC) technique could be a time-saving alternative method for producing doubled haploid (DH) lines that are genetically homozygous. In this study, we successfully utilize the efficient cytokinin thidiazuron (TDZ) to promote microspore embryogenesis and plant regeneration in two curly kale cultivars (‘Winterbor F2’ and ‘Starbor F2’). Compared with the control (0 mg/L TDZ), all tested TDZ concentrations (0.1, 0.2, 0.3, 0.4 mg/L) had no adverse effects on embryogenesis, and 0.2 mg/L TDZ had an optimal effect on embryo survival and plant regeneration of the two genotypes. For ‘Starbor F2’, 0.2 mg/L TDZ treatment achieved the highest embryogenesis rate (1.83-fold higher than the control group) and direct seeding rate (1.61-fold increase), and the lowest mortality rate. Likewise, 0.2 mg/L TDZ increased the embryogenesis rate of ‘Winterbor F2’ by 1.62 times, the direct seeding rate by 1.61 times, and the mortality rate fell to the lowest. A 1/2 Murashige and Skoog (MS) medium with 0.2 mg/L 1-Naphthaleneacetic acid (NAA) can significantly promote the rooting of the regenerated seedlings. These results provide new insights into the practical application of the IMC technique in shortening the breeding cycle of kale.

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Effects of compound sodium nitrophenol on microspore embryogenesis and plantlet regeneration in flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee)

Cited by 2 publications

References 31 publications

Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (Brassica oleracea var. acephala)

Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (Brassica oleracea var. acephala)

Thidiazuron Promoted Microspore Embryogenesis and Plant Regeneration in Curly Kale (Brassica oleracea L. convar. acephala var. sabellica)

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