Effects of Composite LED Light on Root Growth and Antioxidant Capacity of Cunninghamia lanceolata Tissue Culture Seedlings

Xu, Yuanyuan; Liang, Yuyao; Yang, Mei

doi:10.1038/s41598-019-46139-2

Cited by 36 publications

(27 citation statements)

References 45 publications

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“…The plant materials were formally identified by Lecturer Rongyan Deng and deposited in the laboratory of College of Forestry at Guangxi University. The plantlets were pretreated as described by Xu et al [48]. Subsequently, 540 healthy unrooted shoots with consistent growth (approximately 1.5 ± 0.2 cm height) were applied to further LED experiments.…”

Section: Plant Materialsmentioning

confidence: 99%

Effects of LED photoperiods and light qualities on in vitro growth and chlorophyll fluorescence of Cunninghamia lanceolata

Yang

Cheng

et al. 2020

BMC Plant Biol

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Background: Cunninghamia lanceolata (C. lanceolata) is the main fast-growing timber species in southern China. As an alternative to conventional lighting systems, LED has been demonstrated to be an artificial flexible lighting source for commercial micropropagation. The application of LED can provide rapid propagation of C. lanceolata in vitro culture. Results: We applied two-factor randomized block design to study the effects of LED photoperiods and light qualities on the growth and chlorophyll fluorescence of C. lanceolata in vitro culture plantlets. In this study, plantlets were exposed to 20 μmol•m − 2 •s − 1 irradiance for three photoperiods, 8, 16, and 24 h under the three composite lights, 88.9% red+ 11.1% blue (R/B), 80.0% red+ 10.0% blue+ 10.0% purple (R/B/P), 72.7% red+ 9.1% blue+ 9.1% purple+ 9.1% green (R/B/P/G), as well as white light (12.7% red+ 3.9% blue+ 83.4% green, W) as control. The results showed that: (1) Plant height, dry weight, rooting rate, average root number, length, surface area and volume, chlorophyll, and chlorophyll fluorescence parameters were significantly affected by photoperiods, light qualities and their interactions. (2) Plantlets subjected to photoperiod 16 h had longer root, higher height, rooting rate, root number, and the higher levels of chlorophyll, chlorophyll a/b, Y (II), qP, NPQ/4 and ETR II compared to photoperiods 8 h and 24 h, while Fv/Fm during photoperiod 16 h was lower than 8 h and 24 h. Plantlets exposed to R/B/P/G generated more root and presented higher chlorophyll, Fv/Fo, Y (II), qP, and ETR II than W during photoperiods 8 and 16 h. (3) Total chlorophyll content and ETR II were significant correlated with rooting rate, root length and root volume, while Fv/Fm and ETR II were significant correlated with plant height, average root number and root surface area. (4) 16-R/B/P/G is best for growing C. lanceolata plantlets in vitro. Conclusions: This study demonstrated the effectiveness of photoperiods and light qualities using LEDs for micropropagation of C. lanceolata. The best plantlets were harvested under 16-R/B/P/G treatment. And there was a correlation between the growth and the chlorophyll and chlorophyll fluorescence of their leaves under different photoperiod and light quality. These results can contribute to improve the micropropagation process of this species.

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Section: Plant Materialsmentioning

confidence: 99%

Effects of LED photoperiods and light qualities on in vitro growth and chlorophyll fluorescence of Cunninghamia lanceolata

Yang

Cheng

et al. 2020

BMC Plant Biol

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“…For example, significantly improved biomass yield, increased shoot regeneration, and improved adaptability and survival rate of regenerated plants have been reported [ 30 , 31 , 32 , 33 ]. Improved secondary metabolite accumulation and in vitro root growth have been reported by Xu et al [ 34 ] in Cunninghamia lanceolata and Nadeem et al [ 35 ] in Ocimum basilicum under LED irradiation. Plant growth, development, and metabolite production are strongly affected by the light spectrum of the LEDs.…”

Section: Introductionmentioning

confidence: 79%

“…LED irradiation of various cultured plants also increased in vitro adventitious root induction. For example, the adventitious root-promoting effects of LED irradiation were observed in strawberries [ 122 ], chrysanthemum [ 123 ], chestnuts [ 124 ], Oncidium [ 113 ], and C. lanceolata [ 34 ]. The effects of spectral differences in light quality on somatic embryo formation have been reported in Peucedanum japonium [ 125 ], Coffea canephora [ 126 ], Pinus densiflora [ 127 ], Pinus taeda , and Pinus elliottii [ 128 ].…”

Section: Resultsmentioning

confidence: 99%

Application of Light-Emitting Diodes for Improving the Nutritional Quality and Bioactive Compound Levels of Some Crops and Medicinal Plants

et al. 2021

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Light is a key factor that affects phytochemical synthesis and accumulation in plants. Due to limitations of the environment or cultivated land, there is an urgent need to develop indoor cultivation systems to obtain higher yields with increased phytochemical concentrations using convenient light sources. Light-emitting diodes (LEDs) have several advantages, including consumption of lesser power, longer half-life, higher efficacy, and wider variation in the spectral wavelength than traditional light sources; therefore, these devices are preferred for in vitro culture and indoor plant growth. Moreover, LED irradiation of seedlings enhances plant biomass, nutrient and secondary metabolite levels, and antioxidant properties. Specifically, red and blue LED irradiation exerts strong effects on photosynthesis, stomatal functioning, phototropism, photomorphogenesis, and photosynthetic pigment levels. Additionally, ex vitro plantlet development and acclimatization can be enhanced by regulating the spectral properties of LEDs. Applying an appropriate LED spectral wavelength significantly increases antioxidant enzyme activity in plants, thereby enhancing the cell defense system and providing protection from oxidative damage. Since different plant species respond differently to lighting in the cultivation environment, it is necessary to evaluate specific wavebands before large-scale LED application for controlled in vitro plant growth. This review focuses on the most recent advances and applications of LEDs for in vitro culture organogenesis. The mechanisms underlying the production of different phytochemicals, including phenolics, flavonoids, carotenoids, anthocyanins, and antioxidant enzymes, have also been discussed.

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“…Promotion of in vitro root induction and development by red LED treatments has been reported for various plant species, including Tripterospermum japonicum (Moon et al, 2006), grape (Poudel et al, 2008), banana (Wilken et al, 2014), R. glutinosa (Manivannan et al, 2015), and Cunninghamia lanceolata (Xu et al, 2019). The interaction between light quality and cytokinin content in media on multiplication and growth was evaluated during in vitro culture of Myrtus communis L. The results indicated that the highest number of shoots was obtained under red LEDs with the lowest concentration of cytokinin in the media (Cio c et al, 2018).…”

Section: Cytokinin Zmentioning

confidence: 97%

Topolins and Red Light Improve the Micropropagation Efficiency of Passion Fruit (Passiflora edulis Sims) ‘Tainung No. 1’

Chen¹,

Chang²,

Lin³

2020

horts

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Passion fruit is a commercial crop of economic importance worldwide, with recent increases in demand for high-quality plants for commercial production. Plant tissue culture is widely used for the mass propagation of many commercial crops, however its application on passion fruit is challenged by the problem of low reproducibility, leaf chlorosis, and growth retardation resulted from in vitro culture. The aim of this study was to evaluate the effects of cytokinins and light quality on in vitro culture of nodal segments of passion fruit ‘Tainung No. 1’. Three aromatic cytokinins were tested in a modified MS basal medium. The bud proliferation rates of segments initiated on a media containing 1 mg·L−1 meta-topolin riboside (mTR) or benzyladenine (BA) were not significantly different at the same concentration. Buds cultured on medium supplemented with mTR grew and elongated for 4 weeks, while buds on a medium containing BA formed rosettes. After transfer to a medium without plant growth regulators (PGRs), shoots rooted spontaneously within 8 weeks. Furthermore, the effects of continuous propagation under a high proportion of red light affected the subsequent plant growth. Red LED induced an increase in the chlorophyll content (2.71 mg·g−1) compared with other light qualities (1.05–2.63 mg·g−1) and improved plantlet quality. Acclimated plants were grown in the field, and the flower morphology and fruit set were of commercial quality. Findings showed that replacing BA with mTR as the main cytokinin and using a high proportion of red light during the tissue culture induction period produced high-quality plantlets in 3 months. This system is economical and will be further developed for the commercial propagation of passion fruit vines in the future.

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Effects of Composite LED Light on Root Growth and Antioxidant Capacity of Cunninghamia lanceolata Tissue Culture Seedlings

Cited by 36 publications

References 45 publications

Effects of LED photoperiods and light qualities on in vitro growth and chlorophyll fluorescence of Cunninghamia lanceolata

Effects of LED photoperiods and light qualities on in vitro growth and chlorophyll fluorescence of Cunninghamia lanceolata

Application of Light-Emitting Diodes for Improving the Nutritional Quality and Bioactive Compound Levels of Some Crops and Medicinal Plants

Topolins and Red Light Improve the Micropropagation Efficiency of Passion Fruit (Passiflora edulis Sims) ‘Tainung No. 1’

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