Effects of compatibility of deproteinized antler cancellous bone with various bioactive factors on their osteogenic potential

Zhang, Xuehui; Xu, Mingming; Lin, Song; Wei, Yan; Lin, Yucong; Li, Wentao; Heng, Boon Chin; Peng, Hui; Wang, Ying; Deng, Xuliang

doi:10.1016/j.biomaterials.2013.08.024

Cited by 57 publications

(51 citation statements)

References 55 publications

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“…A number of other authors used similar chemical treatment methods to deproteinize bone (NaOCl [5,[11][12][13][14][15][16]22,24], [21,23,25], KOH [18,19], NaOH [21,3]), and applied the same [5,11,13,18,19,23] or different concentrations of the chemical agent solutions [12,[14][15][16][21][22][23][24][25]. These chemical solutions were applied as individual deproteinization agents [5,11,[12][13][14][15][16]18,19,22] or as one of the stages in more complex deproteinization processes [21,23].…”

Section: Resultsmentioning

confidence: 99%

“…The course of all deproteinization processes was periodically assessed by measuring the protein concentration in bone specimens using the Lowry method [21]. For this purpose, directly before the beginning of the deproteinization process, as well as after the 1 st , 2 nd , 3 rd , 5 th , 7 th 12 th and 14 th days of the process, two bone specimens were taken from each deproteinizing agent.…”

Section: Deproteinization Processesmentioning

confidence: 99%

“…A variety of bone deproteinization methods were the subject of studies carried out by a number of other authors. Although some of the deproteinization agents or treatments were commonly used by these authors for bone deproteinization, the other deproteinization agents or treatments were incidentally applied by various authors in their specific research on the deproteinized bone specimens [2,5,[12][13][14][15][16][17][18][19][20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

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Effectiveness of various deproteinization processes of bovine cancellous bone evaluated via mechano-biostructural properties of produced osteoconductive biomaterials

Uklejewski

Winiecki

Musielak

et al. 2015

Biotechnol Bioproc E

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The purpose of this work was to characterize the results of five different processes of bovine tissue deproteinization, resulting in the fabrication of deproteinized xenogenic osteoconductive biomaterials for bone tissue regeneration as an alternative to autogenic bone grafts. The studies on deproteinization processes of bovine cancellous bone specimens, excised from fresh femoral bovine heads, included the following five processes: thermal deproteinization and four chemical deproteinization processes using the solutions 2.6 wt% sodium hypochlorite, 7 wt% hydrogen peroxide, 1 N potassium hydroxide and 1 N sodium hydroxide. The optimal parameters of the thermal deproteinization were designed on the basis of thermogravimetric analysis (TGA) of bovine bone specimens during a pilot study of the process. Periodical evaluation of all the processes of chemical deproteinization was performed via the assessment of protein concentration in bone specimens by the Lowry method. The mechanical properties of deproteinized bone specimens were evaluated by compression testing in the air-dry condition. The compressive strength of the completely deproteinized bone specimens after the thermal deproteinization process was remarkably lower compared with those after the chemical deproteinization processes: 6.5 times lower compared with those deproteinized with 7 wt% hydrogen peroxide solution and 3 times lower compared with those deproteinized with 2.6 wt% sodium hypochlorite solution. The SEM examination of deproteinized bone specimens showed microcracks on the trabecular surfaces generated by thermal deproteinization stresses. The presence of microcracks in the biomaterial resulted in a decrease in its ultimate compressive strength.

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Section: Resultsmentioning

confidence: 99%

Section: Deproteinization Processesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effectiveness of various deproteinization processes of bovine cancellous bone evaluated via mechano-biostructural properties of produced osteoconductive biomaterials

Uklejewski

Winiecki

Musielak

et al. 2015

Biotechnol Bioproc E

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“…16 bottom). Concerning biomedical applications, the initial attempts to evaluate a potential use of deer antlers as xenogenic bone grafts have been already performed (Baciut et al 2007;Hasan et al 2012;Zhang et al 2012Zhang et al , 2013. In the body of mammals, osteoblasts and odontoblasts fix ions of calcium and orthophosphate and then precipitate biological apatite onto an organic matrix.…”

Section: Antlersmentioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

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“…The main effect of IL-7 is to maintain the survival of memory T cells, while IL-15 plays an important role in the process of T memory cell proliferation (White et al, 2016;Zaunders, Lévy, & Seddiki, 2014). Although many cytokines (TNF-α, IL-6, IL-12, MMP-2, MMP-9) are regulated by pilose antler treatment (Dai et al, 2011;Kim et al, 2012;Zhang et al, 2013), there is no relevant report on whether pilose antler promotes IL-7 and IL-15 synthesis in vivo. CD4+ T cells also play an important role in maintaining the content and function of TEM (de Oliveira Mendes-Aguiar et al, 2016).…”

mentioning

confidence: 99%

Distinctive effects of pilose antler on mouse peripheral blood immune cell populations

Zheng

Fan

et al. 2017

Food and Agricultural Immunology

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Pilose antler is a rare animal-sourced traditional Chinese medicine that has been demonstrated to cause a change in total immunity. Little is known about its specific effect on immune cells. In this study, the immunological modulatory effects of pilose antler extract on mice were investigated. Mice were gavaged daily for 7 days with pilose antler extracts, and peripheral blood was analysed by flow cytometry. Our results show that pilose antler extract exerted distinctive effects on the different immune cell types analysed. Pilose antler gavage transiently increased T cell percentage and decreased B cell percentage in peripheral blood. After treatment, the proliferation of killer and helper T cells was stimulated in the short term, but in the longer term, NK and memory T cell populations were increased. These results suggest that pilose antler treatment has both transient and long-term effects on the immune system in mice.ARTICLE HISTORY

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Effects of compatibility of deproteinized antler cancellous bone with various bioactive factors on their osteogenic potential

Cited by 57 publications

References 55 publications

Effectiveness of various deproteinization processes of bovine cancellous bone evaluated via mechano-biostructural properties of produced osteoconductive biomaterials

Effectiveness of various deproteinization processes of bovine cancellous bone evaluated via mechano-biostructural properties of produced osteoconductive biomaterials

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Distinctive effects of pilose antler on mouse peripheral blood immune cell populations

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